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Signal Transduction Signaling Pathway Nuclear Signaling STATs
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RecombinantRabMAb

Recombinant PE Anti-STAT6 antibody [YE361] (ab223917)

  • Datasheet
  • Certificate of Compliance
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Flow Cytometry (Intracellular) - PE Anti-STAT6 antibody [YE361] (ab223917)
  • PE Anti-STAT6 antibody [YE361] (ab223917)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • PE Rabbit monoclonal [YE361] to STAT6
  • Suitable for: Flow Cyt (Intra)
  • Reacts with: Mouse
  • Conjugation: PE. Ex: 488nm, Em: 575nm

Conjugates logo Related conjugates and formulations

Alexa Fluor® 405 Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 APC Carrier Free FITC HRP Unconjugated

You may also be interested in

Protein
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Recombinant Human STAT6 protein (ab125625)
Primary
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Anti-STAT6 antibody [YE361] - BSA and Azide free (ab215995)
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Alexa Fluor® 647 Anti-CD161 antibody [EPR23831-120] (ab281735)

View more associated products

Overview

  • Product name

    PE Anti-STAT6 antibody [YE361]
    See all STAT6 primary antibodies
  • Description

    PE Rabbit monoclonal [YE361] to STAT6
  • Host species

    Rabbit
  • Conjugation

    PE. Ex: 488nm, Em: 575nm
  • Tested applications

    Suitable for: Flow Cyt (Intra)more details
  • Species reactivity

    Reacts with: Mouse
    Predicted to work with: Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Flow Cyt (intra): NIH3T3 cells
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at +4°C. Do Not Freeze. Store In the Dark.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 1% BSA, PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    YE361
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Signaling Pathway
    • Nuclear Signaling
    • STATs
    • Epigenetics and Nuclear Signaling
    • Nuclear Signaling Pathways
    • STATs
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Transcription Factors
    • Cancer
    • Signal transduction
    • Nuclear signaling
    • STAT family
    • Kits/ Lysates/ Other
    • Kits
    • ELISA Kits
    • In Cell ELISA Kits
    • Phosphoprotein ICE kits

Associated products

  • Alternative Versions

    • Alexa Fluor® 488 Anti-STAT6 antibody [YE361] (ab196478)
    • HRP Anti-STAT6 antibody [YE361] (ab196480)
    • Alexa Fluor® 647 Anti-STAT6 antibody [YE361] (ab196928)
    • Alexa Fluor® 594 Anti-STAT6 antibody [YE361] (ab207012)
    • Alexa Fluor® 405 Anti-STAT6 antibody [YE361] (ab207013)
    • Alexa Fluor® 555 Anti-STAT6 antibody [YE361] (ab207014)
    • Alexa Fluor® 568 Anti-STAT6 antibody [YE361] (ab207015)
    • Anti-STAT6 antibody [YE361] - BSA and Azide free (ab215995)
    • FITC Anti-STAT6 antibody [YE361] (ab223918)
    • APC Anti-STAT6 antibody [YE361] (ab223919)
    • Anti-STAT6 antibody [YE361] (ab32520)
  • Isotype control

    • PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab209478)
  • Recombinant Protein

    • Recombinant Human STAT6 protein (ab125625)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab223917 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/1000.

The cellular localisation of this product has been verified in ICC/IF.

Notes
Flow Cyt (Intra)
1/1000.

The cellular localisation of this product has been verified in ICC/IF.

Target

  • Function

    Carries out a dual function: signal transduction and activation of transcription. Involved in interleukin-4 signalling.
  • Sequence similarities

    Belongs to the transcription factor STAT family.
    Contains 1 SH2 domain.
  • Post-translational
    modifications

    Tyrosine phosphorylated following stimulation by IL-4 and IL-3.
  • Cellular localization

    Cytoplasm. Nucleus. Translocated into the nucleus in response to phosphorylation.
  • Target information above from: UniProt accession P42226 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 6778 Human
    • Entrez Gene: 20852 Mouse
    • Omim: 601512 Human
    • SwissProt: P42226 Human
    • SwissProt: P52633 Mouse
    • Unigene: 524518 Human
    • Alternative names

      • 12S1644 antibody
      • D12S1644 antibody
      • IL 4 STAT antibody
      • IL-4 Stat antibody
      • IL4 STAT antibody
      • Interleukin 4 Induced antibody
      • Interleukin 4 Induced Transcription Factor IL4 STAT antibody
      • Signal transducer and activator of transcription 6 antibody
      • Signal Transducer And Activator Of Transcription 6 Interleukin 4 Induced antibody
      • Signal Transducer And Activator Of Transcription 6 Nirs Variant 1 antibody
      • Signal transducer and activator of transcription 6, interleukin 4 induced antibody
      • STAT 6 antibody
      • STAT interleukin4 induced antibody
      • STAT, interleukin4 induced antibody
      • Stat6 antibody
      • STAT6_HUMAN antibody
      • STAT6B antibody
      • STAT6C antibody
      • Transcription factor IL 4 STAT antibody
      see all

    Images

    • Flow Cytometry (Intracellular) - PE Anti-STAT6 antibody [YE361] (ab223917)
      Flow Cytometry (Intracellular) - PE Anti-STAT6 antibody [YE361] (ab223917)

      Overlay histogram showing NIH3T3 cells stained with ab223917 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab223917, 1/1000 dilution) for 30 min at 22°C.

      Isotype control antibody (black line) was Rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

      Acquisition of >5,000 events were collected using a 50 mW Yellow/Green laser (561nm) and 586/15 bandpass filter.

      This antibody gave a positive signal in NIH3T3 cells fixed with 4% formaldehyde (10 min)/permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.

    • PE Anti-STAT6 antibody [YE361] (ab223917)
      PE Anti-STAT6 antibody [YE361] (ab223917)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

    References (0)

    Publishing research using ab223917? Please let us know so that we can cite the reference in this datasheet.

    ab223917 has not yet been referenced specifically in any publications.

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