RecombinantRabMAb

Recombinant PE Anti-Syntenin antibody [EPR8102] (ab210837)

Submit a review Submit a question
Flow Cytometry - PE Anti-Syntenin antibody [EPR8102] (ab210837)
  • Immunocytochemistry/ Immunofluorescence - PE Anti-Syntenin antibody [EPR8102] (ab210837)
  • PE Anti-Syntenin antibody [EPR8102] (ab210837)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • PE Rabbit monoclonal [EPR8102] to Syntenin
  • Suitable for: Flow Cyt, ICC/IF
  • Reacts with: Human
  • Conjugation: PE. Ex: 488nm, Em: 575nm

Overview

  • Product name

    PE Anti-Syntenin antibody [EPR8102]
    See all Syntenin primary antibodies

  • Description

    PE Rabbit monoclonal [EPR8102] to Syntenin

  • Host species

    Rabbit

  • Conjugation

    PE. Ex: 488nm, Em: 575nm

  • Tested applications

    Suitable for: Flow Cyt, ICC/IFmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human Syntenin aa 1-100. The exact sequence is proprietary.
    Database link: O00560

  • Positive control

    • Flow Cyt: HeLa cells ICC/IF: HeLa cells

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab210837 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/5000.
ICC/IF 1/100.

This product gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min)

Target

  • Function

    Seems to function as an adapter protein. In adherens junctions may function to couple syndecans to cytoskeletal proteins or signaling components. Seems to couple transcription factor SOX4 to the IL-5 receptor (IL5RA). May also play a role in vesicular trafficking. Seems to be required for the targeting of TGFA to the cell surface in the early secretory pathway.

  • Tissue specificity

    Widely expressed. Expressed in fetal kidney, liver, lung and brain. In adult highest expression in heart and placenta.

  • Sequence similarities

    Contains 2 PDZ (DHR) domains.

  • Post-translational
    modifications

    Phosphorylated on tyrosine residues.

  • Cellular localization

    Cell junction > focal adhesion. Cell junction > adherens junction. Cell membrane. Endoplasmic reticulum membrane. Nucleus. Melanosome. Cytoplasm > cytosol. Cytoplasm > cytoskeleton. Mainly membrane-associated. Localized to adherens junctions, focal adhesions and endoplasmic reticulum. Colocalized with actin stress fibers. Also found in the nucleus. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt

  • Database links

    • Alternative names

      • MDA-9 antibody
      • MDA9 antibody
      • Melanoma differentiation-associated protein 9 antibody
      • Pro-TGF-alpha cytoplasmic domain-interacting protein 18 antibody
      • Scaffold protein Pbp1 antibody
      • SDCB1_HUMAN antibody
      • SDCBP antibody
      • ST1 antibody
      • SYCL antibody
      • Syndecan binding protein (syntenin) antibody
      • Syndecan binding protein 1 antibody
      • Syndecan-binding protein 1 antibody
      • Syntenin 1 antibody
      • Syntenin-1 antibody
      • TACIP18 antibody
      see all

    Images

    • Flow Cytometry - PE Anti-Syntenin antibody [EPR8102] (ab210837)
      Flow Cytometry - PE Anti-Syntenin antibody [EPR8102] (ab210837)

      Overlay histogram showing HeLa cells stained with ab210837 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 90% methanol for 30 min at -20°C. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab210837, 1/5000 dilution) for 30 min at 22°C.

      Isotype control antibody (black line) was rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

      Acquisition of >5,000 events were collected using a 50mW Yellow-Green laser (561nm) and 586/15 bandpass filter.

    • Immunocytochemistry/ Immunofluorescence - PE Anti-Syntenin antibody [EPR8102] (ab210837)
      Immunocytochemistry/ Immunofluorescence - PE Anti-Syntenin antibody [EPR8102] (ab210837)

      ab210837 staining Syntenin in HeLa cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab210837 at 1/100 dilution (pseudocolored in green) and ab195884, Rat monoclonal to Tubulin (Alexa Fluor® 647), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

      Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

      This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 4% formaldehyde (10 min).

    • PE Anti-Syntenin antibody [EPR8102] (ab210837)
      PE Anti-Syntenin antibody [EPR8102] (ab210837)

    References (0)

    Publishing research using ab210837? Please let us know so that we can cite the reference in this datasheet.

    ab210837 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    There are currently no Customer reviews or Questions for ab210837.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com