RecombinantRabMAb

Recombinant PE Anti-SYVN1/HRD1 antibody [EP7459] (ab211705)

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Flow Cytometry - PE Anti-SYVN1/HRD1 antibody [EP7459] (ab211705)
  • PE Anti-SYVN1/HRD1 antibody [EP7459] (ab211705)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • PE Rabbit monoclonal [EP7459] to SYVN1/HRD1
  • Suitable for: Flow Cyt
  • Reacts with: Human
  • Conjugation: PE. Ex: 488nm, Em: 575nm

Overview

  • Product name

    PE Anti-SYVN1/HRD1 antibody [EP7459]
    See all SYVN1/HRD1 primary antibodies

  • Description

    PE Rabbit monoclonal [EP7459] to SYVN1/HRD1

  • Host species

    Rabbit

  • Conjugation

    PE. Ex: 488nm, Em: 575nm

  • Tested applications

    Suitable for: Flow Cytmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Synthetic peptide within Human SYVN1/HRD1 aa 150-250 (internal sequence) (Cysteine residue). The exact sequence is proprietary.
    Database link: Q86TM6

  • Positive control

    • Flow Cyt: HeLa cells.

  • General notes

     

     

     

     This product was previously labelled as SYVN1

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab211705 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/2500.

The cellular localisation of this product has been verified in ICC/IF.

Target

  • Function

    Acts as an E3 ubiquitin-protein ligase which accepts ubiquitin specifically from endoplasmic reticulum-associated UBC7 E2 ligase and transfers it to substrates, promoting their degradation. Component of the endoplasmic reticulum quality control (ERQC) system also called ER-associated degradation (ERAD) involved in ubiquitin-dependent degradation of misfolded endoplasmic reticulum proteins. Also promotes the degradation of normal but naturally short-lived proteins such as SGK. Protects cells from ER stress-induced apoptosis. Protects neurons from apoptosis induced by polyglutamine-expanded huntingtin (HTT) or unfolded GPR37 by promoting their degradation. Sequesters p53/TP53 in the cytoplasm and promotes its degradation, thereby negatively regulating its biological function in transcription, cell cycle regulation and apoptosis.

  • Tissue specificity

    Ubiquitously expressed, with highest levels in liver and kidney (at protein level). Up-regulated in synovial tissues from patients with rheumatoid arthritis (at protein level).

  • Pathway

    Protein modification; protein ubiquitination.

  • Sequence similarities

    Belongs to the HRD1 family.
    Contains 1 RING-type zinc finger.

  • Domain

    The RING-type zinc finger is required for E3 ligase activity.

  • Post-translational
    modifications

    Not N-glycosylated.
    Auto-ubiquitinated.

  • Cellular localization

    Endoplasmic reticulum membrane.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • 1200010C09Rik antibody
    • DER3 antibody
    • E3 ubiquitin-protein ligase synoviolin antibody
    • HMG coA reductase degradation 1 homolog antibody
    • HRD1 antibody
    • KIAA1810 antibody
    • MGC40372 antibody
    • OTTHUMP00000230429 antibody
    • OTTHUMP00000230430 antibody
    • OTTHUMP00000230431 antibody
    • OTTHUMP00000230432 antibody
    • Synovial apoptosis inhibitor 1 antibody
    • Synovial apoptosis inhibitor 1, synoviolin antibody
    • Synoviolin 1 antibody
    • Synoviolin 1 isoform b antibody
    • SYNOVIOLIN antibody
    • SYVN1 antibody
    • SYVN1_HUMAN antibody
    see all

Images

  • Flow Cytometry - PE Anti-SYVN1/HRD1 antibody [EP7459] (ab211705)
    Flow Cytometry - PE Anti-SYVN1/HRD1 antibody [EP7459] (ab211705)
    Overlay histogram showing HeLa cells stained with ab211705 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab211705, 1/2500 dilution) for 30 min at 22°C.

    Isotype control antibody (black line) was rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

    Acquisition of >5,000 events were collected using a 50 mW Yellow/Green laser (561nm) and 586/15 bandpass filter.

    This antibody gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min)/permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.
  • PE Anti-SYVN1/HRD1 antibody [EP7459] (ab211705)
    PE Anti-SYVN1/HRD1 antibody [EP7459] (ab211705)

References (0)

Publishing research using ab211705? Please let us know so that we can cite the reference in this datasheet.

ab211705 has not yet been referenced specifically in any publications.

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