Recombinant PE Anti-SYVN1/HRD1 antibody [EP7459] (ab211705)
![Flow Cytometry (Intracellular) - PE Anti-SYVN1/HRD1 antibody [EP7459] (ab211705)](https://www.abcam.com/ps/products/211/ab211705/Images/ab211705-2-ab211705FLOW1.jpg "Flow Cytometry (Intracellular) - PE Anti-SYVN1/HRD1 antibody [EP7459] (ab211705)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- PE Rabbit monoclonal [EP7459] to SYVN1/HRD1
- Suitable for: Flow Cyt (Intra)
- Reacts with: Human
- Conjugation: PE. Ex: 488nm, Em: 575nm
Related conjugates and formulations
Overview
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Product name
PE Anti-SYVN1/HRD1 antibody [EP7459]
See all SYVN1/HRD1 primary antibodies -
Description
PE Rabbit monoclonal [EP7459] to SYVN1/HRD1 -
Host species
Rabbit -
Conjugation
PE. Ex: 488nm, Em: 575nm -
Tested applications
Suitable for: Flow Cyt (Intra)more details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Flow Cyt (intra): HeLa cells.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at 4°C (stable for up to 12 months). Upon delivery aliquot. Store at +4°C. Do Not Freeze. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EP7459 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab211705 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/2500.
The cellular localisation of this product has been verified in ICC/IF. |
| Notes |
|---|
|
Flow Cyt (Intra)
1/2500. The cellular localisation of this product has been verified in ICC/IF. |
Target
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Function
Acts as an E3 ubiquitin-protein ligase which accepts ubiquitin specifically from endoplasmic reticulum-associated UBC7 E2 ligase and transfers it to substrates, promoting their degradation. Component of the endoplasmic reticulum quality control (ERQC) system also called ER-associated degradation (ERAD) involved in ubiquitin-dependent degradation of misfolded endoplasmic reticulum proteins. Also promotes the degradation of normal but naturally short-lived proteins such as SGK. Protects cells from ER stress-induced apoptosis. Protects neurons from apoptosis induced by polyglutamine-expanded huntingtin (HTT) or unfolded GPR37 by promoting their degradation. Sequesters p53/TP53 in the cytoplasm and promotes its degradation, thereby negatively regulating its biological function in transcription, cell cycle regulation and apoptosis. -
Tissue specificity
Ubiquitously expressed, with highest levels in liver and kidney (at protein level). Up-regulated in synovial tissues from patients with rheumatoid arthritis (at protein level). -
Pathway
Protein modification; protein ubiquitination. -
Sequence similarities
Belongs to the HRD1 family.
Contains 1 RING-type zinc finger. -
Domain
The RING-type zinc finger is required for E3 ligase activity. -
Post-translational
modificationsNot N-glycosylated.
Auto-ubiquitinated. -
Cellular localization
Endoplasmic reticulum membrane. - Information by UniProt
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Database links
- Entrez Gene: 84447 Human
- Entrez Gene: 74126 Mouse
- Entrez Gene: 361712 Rat
- Omim: 608046 Human
- SwissProt: Q86TM6 Human
- SwissProt: Q9DBY1 Mouse
- Unigene: 75859 Human
- Unigene: 149870 Mouse
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Alternative names
- 1200010C09Rik antibody
- DER3 antibody
- E3 ubiquitin-protein ligase synoviolin antibody
see all
Images
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Flow Cytometry (Intracellular) - PE Anti-SYVN1/HRD1 antibody [EP7459] (ab211705)Overlay histogram showing HeLa cells stained with ab211705 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab211705, 1/2500 dilution) for 30 min at 22°C.
Isotype control antibody (black line) was rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 50 mW Yellow/Green laser (561nm) and 586/15 bandpass filter.
This antibody gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min)/permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions. -
![PE Anti-SYVN1/HRD1 antibody [EP7459] (ab211705)](https://www.abcam.com/ps/products/211/ab211705/Images/ab211705-3-benefits-of-recombinant-antibodies.png)
PE Anti-SYVN1/HRD1 antibody [EP7459] (ab211705)
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab211705 has not yet been referenced specifically in any publications.
