PE Anti-TCR gamma + TCR delta antibody [UC7-13D5] (ab25663)
![Flow Cytometry - PE Anti-TCR gamma + TCR delta antibody [UC7-13D5] (ab25663)](https://www.abcam.com/ps/products/25/ab25663/Images/ab25663-1-anti-tcr-gamma-tcr-delta-antibody-uc7-13d5-pe-flow-cytometry-lymph-node-c57bl-6-mouse.jpg "Flow Cytometry - PE Anti-TCR gamma + TCR delta antibody [UC7-13D5] (ab25663)")
Key features and details
- PE Armenian hamster monoclonal [UC7-13D5] to TCR gamma + TCR delta
- Reacts with: Mouse
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Isotype: IgG
Overview
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Product name
PE Anti-TCR gamma + TCR delta antibody [UC7-13D5]
See all TCR gamma + TCR delta primary antibodies -
Description
PE Armenian hamster monoclonal [UC7-13D5] to TCR gamma + TCR delta -
Host species
Armenian hamster -
Conjugation
PE. Ex: 488nm, Em: 575nm -
Specificity
ab25663 is specific for the gamma-delta heterodimer and plate-bound ab25663 activates gamma-delta TRC-bearing cells. This antibody does not react with alpha-beta TCR-expressing T cells. -
Species reactivity
Reacts with: Mouse -
Immunogen
The details of the immunogen for this antibody are not available.
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Positive control
- Flow Cyt: C57BL/6 mouse mesenteric lymph node cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Store at +4°C in the dark. Do not freeze. -
Storage buffer
pH: 7.3
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
UC7-13D5 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
Cellular depletion (In vivo depletion of γδ TCR-expressing T cells): Use at an assay dependent dilution.
Flow Cyt: Use 0.3µg for 106 cells.
IHC-Fr: Use at an assay dependent dilution.
IP: Use at an assay dependent dilution.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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Relevance
T cell receptors (TCR) recognize foreign antigens which have been processed as small peptides and bound to major histocompatibility complex (MHC) molecules at the surface of antigen presenting cells (APC). Each T cell receptor is a dimer consisting of one a and one b chain or one d and one g chain. This region represents the germline organization of the T cell receptor beta locus. The beta locus includes V (variable), J (joining), diversity (D), and C (constant) segments. During T cell development, the beta chain is synthesized by a recombination event at the DNA level joining a D segment with a J segment; a V segment is then joined to the D-J gene. The C segment is later joined by splicing at the RNA level. The g/d TCR associates with CD3 and is expressed on a T cell subset found in the thymus, the intestinal epithelium, and the peripheral lymphoid tissues and peritoneum. Most g/d T cells are CD4-/CD8-, some are CD8+. T cells expressing the g/d TCR have been shown to play a role in oral tolerance, tumor-associated tolerance, and autoimmune disease. -
Cellular localization
Type I membrane protein -
Database links
- Entrez Gene: 12500 Mouse
- Entrez Gene: 12502 Mouse
- SwissProt: P04235 Mouse
- SwissProt: P11942 Mouse
- Unigene: 335106 Mouse
- Unigene: 4527 Mouse
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Alternative names
- CD_antigen=CD3d antibody
- CD3D antibody
- CD3G antibody
see all
Images
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Flow Cytometry - PE Anti-TCR gamma + TCR delta antibody [UC7-13D5] (ab25663)
Flow cytometric analysis of C57BL/6 mouse mesenteric lymph node cells labeling TCR gamma + TCR delta using ab25663 at 0.3 µg/106 cells. CD3ε is labeled using a Rat Anti-Mouse CD3ε (FITC) antibody.
Protocols
Datasheets and documents
References (4)
ab25663 has been referenced in 4 publications.
- Bluestone JA et al. Repertoire development and ligand specificity of murine TCR gamma delta cells. Immunol Rev 120:5-33 (1991). PubMed: 1650760
- Barrett TA et al. Control of self-reactivity in the intestine. Immunol Res 10:341-8 (1991). PubMed: 1835488
- Dent AL et al. Self-reactive gamma delta T cells are eliminated in the thymus. Nature 343:714-9 (1990). PubMed: 1968227
- Houlden BA et al. A TCR gamma delta cell recognizing a novel TL-encoded gene product. Cold Spring Harb Symp Quant Biol 54 Pt 1:45-55 (1989). PubMed: 2576978
