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  1. Link

    pe-tdp43-antibody-epr18554-ab237272.pdf

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Microbiology Organism Virus RNA Virus ssRNA positive strand virus HIV
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RecombinantRabMAb

Recombinant PE Anti-TDP43 antibody [EPR18554] (ab237272)

  • Datasheet
  • Certificate of Compliance
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Flow Cytometry (Intracellular) - PE Anti-TDP43 antibody [EPR18554] (ab237272)
  • PE Anti-TDP43 antibody [EPR18554] (ab237272)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • PE Rabbit monoclonal [EPR18554] to TDP43
  • Suitable for: Flow Cyt (Intra)
  • Reacts with: Human
  • Conjugation: PE. Ex: 488nm, Em: 575nm

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 647 Carrier Free Unconjugated

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Conjugation
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PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
Protein
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Recombinant Human TDP43 protein (denatured) (ab156345)
Primary
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Alexa Fluor® 488 Anti-Superoxide Dismutase 1 antibody [EP1727Y] (ab199733)

View more associated products

Overview

  • Product name

    PE Anti-TDP43 antibody [EPR18554]
    See all TDP43 primary antibodies
  • Description

    PE Rabbit monoclonal [EPR18554] to TDP43
  • Host species

    Rabbit
  • Conjugation

    PE. Ex: 488nm, Em: 575nm
  • Tested applications

    Suitable for: Flow Cyt (Intra)more details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Flow Cyt (intra): K562 cells
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at +4°C. Store In the Dark.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 1% BSA, PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR18554
  • Isotype

    IgG
  • Research areas

    • Microbiology
    • Organism
    • Virus
    • RNA Virus
    • ssRNA positive strand virus
    • HIV
    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Amyotrophic lateral sclerosis
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • RNA Processing
    • Splicing
    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Other
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Other factors
    • Neuroscience
    • Diseases

Associated products

  • Alternative Versions

    • Anti-TDP43 antibody [EPR18554] (ab190963)
    • Alexa Fluor® 488 Anti-TDP43 antibody [EPR18554] (ab237270)
    • Alexa Fluor® 647 Anti-TDP43 antibody [EPR18554] (ab237271)
    • Anti-TDP43 antibody [EPR18554] - BSA and Azide free (ab238443)
  • Recombinant Protein

    • Recombinant Human TDP43 protein (denatured) (ab156345)
  • Related Products

    • PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab209478)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab237272 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/1250.
Notes
Flow Cyt (Intra)
1/1250.

Target

  • Function

    DNA and RNA-binding protein which regulates transcription and splicing. Involved in the regulation of CFTR splicing. It promotes CFTR exon 9 skipping by binding to the UG repeated motifs in the polymorphic region near the 3'-splice site of this exon. The resulting aberrant splicing is associated with pathological features typical of cystic fibrosis. May also be involved in microRNA biogenesis, apoptosis and cell division. Can repress HIV-1 transcription by binding to the HIV-1 long terminal repeat. Stabilizes the low molecular weight neurofilament (NFL) mRNA through a direct interaction with the 3' UTR.
  • Tissue specificity

    Ubiquitously expressed. In particular, expression is high in pancreas, placenta, lung, genital tract and spleen.
  • Involvement in disease

    Defects in TARDBP are the cause of amyotrophic lateral sclerosis type 10 (ALS10) [MIM:612069]. ALS is a neurodegenerative disorder affecting upper and lower motor neurons and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology of ALS is likely to be multifactorial, involving both genetic and environmental factors. The disease is inherited in 5-10% of the cases.
  • Sequence similarities

    Contains 2 RRM (RNA recognition motif) domains.
  • Domain

    The RRM domains can bind to both DNA and RNA.
  • Post-translational
    modifications

    Hyperphosphorylated in hippocampus, neocortex, and spinal cord from individuals affected with ALS and FTLDU.
    Ubiquitinated in hippocampus, neocortex, and spinal cord from individuals affected with ALS and FTLDU.
    Cleaved to generate C-terminal fragments in hippocampus, neocortex, and spinal cord from individuals affected with ALS and FTLDU.
  • Cellular localization

    Nucleus. In patients with frontotemporal lobar degeneration and amyotrophic lateral sclerosis, it is absent from the nucleus of affected neurons but it is the primary component of cytoplasmic ubiquitin-positive inclusion bodies.
  • Target information above from: UniProt accession Q13148 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 23435 Human
    • Omim: 605078 Human
    • SwissProt: Q13148 Human
    • Unigene: 300624 Human
    • Unigene: 635053 Human
    • Alternative names

      • ALS10 antibody
      • OTTHUMP00000002171 antibody
      • OTTHUMP00000002172 antibody
      • OTTHUMP00000002173 antibody
      • TADBP_HUMAN antibody
      • TAR DNA binding protein 43 antibody
      • TAR DNA binding protein antibody
      • TAR DNA-binding protein 43 antibody
      • TARDBP antibody
      • TDP 43 antibody
      • TDP-43 antibody
      • TDP43 antibody
      see all

    Images

    • Flow Cytometry (Intracellular) - PE Anti-TDP43 antibody [EPR18554] (ab237272)
      Flow Cytometry (Intracellular) - PE Anti-TDP43 antibody [EPR18554] (ab237272)

      Overlay histogram showing K562 cells stained with ab237272 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal Goat serum to block non-specific protein-protein interactions followed by the antibody (ab237272, 1/1250 dilution) for 30 min at 22°C. Isotype control antibody (black line) was Rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabeled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 50 mW Yellow/Green laser (561nm) and 586/15 bandpass filter. This antibody gave a positive signal in K562  cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.

    • PE Anti-TDP43 antibody [EPR18554] (ab237272)
      PE Anti-TDP43 antibody [EPR18554] (ab237272)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

    References (0)

    Publishing research using ab237272? Please let us know so that we can cite the reference in this datasheet.

    ab237272 has not yet been referenced specifically in any publications.

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