Recombinant PE Anti-Thymidylate Synthase antibody [EPR4545] (ab225092)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- PE Rabbit monoclonal [EPR4545] to Thymidylate Synthase
- Suitable for: Flow Cyt (Intra)
- Reacts with: Human
- Conjugation: PE. Ex: 488nm, Em: 575nm
Related conjugates and formulations
Overview
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Product name
PE Anti-Thymidylate Synthase antibody [EPR4545]
See all Thymidylate Synthase primary antibodies -
Description
PE Rabbit monoclonal [EPR4545] to Thymidylate Synthase -
Host species
Rabbit -
Conjugation
PE. Ex: 488nm, Em: 575nm -
Tested applications
Suitable for: Flow Cyt (Intra)more details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Flow Cyt (intra): HeLa cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at +4°C. Do Not Freeze. Store In the Dark. -
Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR4545 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
- Anti-Thymidylate Synthase antibody [EPR4545] (ab108995)
- Alexa Fluor® 488 Anti-Thymidylate Synthase antibody [EPR4545] (ab197898)
- Alexa Fluor® 647 Anti-Thymidylate Synthase antibody [EPR4545] (ab197899)
- APC Anti-Thymidylate Synthase antibody [EPR4545] (ab225091)
- Anti-Thymidylate Synthase antibody [EPR4545] - BSA and Azide free (ab232021)
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab225092 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt (Intra) |
1/1000.
The cellular localisation of this product has been verified in ICC/IF. |
Notes |
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Flow Cyt (Intra)
1/1000. The cellular localisation of this product has been verified in ICC/IF. |
Target
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Function
Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. -
Pathway
Pyrimidine metabolism; dTTP biosynthesis. -
Sequence similarities
Belongs to the thymidylate synthase family. -
Cellular localization
Nucleus. Cytoplasm. Mitochondrion. Mitochondrion matrix. Mitochondrion inner membrane. - Information by UniProt
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Database links
- Entrez Gene: 7298 Human
- Entrez Gene: 22171 Mouse
- Entrez Gene: 29261 Rat
- Omim: 188350 Human
- SwissProt: P04818 Human
- SwissProt: P07607 Mouse
- SwissProt: P45352 Rat
- Unigene: 369762 Human
see all -
Alternative names
- d TMP synthase antibody
- EC 2.1.1.45 antibody
- HsT422 antibody
see all
Images
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Overlay histogram showing HeLa cells stained with ab225092 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab225092, 1/1000 dilution) for 30 min at 22°C.
Isotype control antibody (black line) was Rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 50 mW Yellow/Green laser (561nm) and 586/15 bandpass filter.
This antibody gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min)/permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab225092 has not yet been referenced specifically in any publications.