PE Anti-TLR3 antibody [40C1285.6] (ab45093)

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Flow Cytometry - PE Anti-TLR3 antibody [40C1285.6] (ab45093)
  • Flow Cytometry - PE Anti-TLR3 antibody [40C1285.6] (ab45093)

Key features and details

  • PE Mouse monoclonal [40C1285.6] to TLR3
  • Suitable for: Flow Cyt
  • Reacts with: Human
  • Conjugation: PE. Ex: 488nm, Em: 575nm
  • Isotype: IgG1

Overview

  • Product name

    PE Anti-TLR3 antibody [40C1285.6]
    See all TLR3 primary antibodies

  • Description

    PE Mouse monoclonal [40C1285.6] to TLR3

  • Host species

    Mouse

  • Conjugation

    PE. Ex: 488nm, Em: 575nm

  • Tested applications

    Suitable for: Flow Cytmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    This antibody was developed against KLH-conjugated synthetic peptide of human TLR3.

  • Positive control

    • Human intestine or Ramos.

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab45093 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
  • Application notes
    FACS:(Intracellular) Use 2 - 5µg for 106 cells.
    WB: Use at a concentration of 1 - 3 µg/ml. Predicted molecular weight: 104 kDa.

    This antibody has tested negative by western blot in mouse intestine cell lysate.



    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • Function

      Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific of microorganisms. TLR3 is a nucleotide-sensing TLR which is activated by double-stranded RNA, a sign of viral infection. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.

    • Tissue specificity

      Expressed at high level in placenta and pancreas. Also detected in CD11c+ immature dendritic cells. Only expressed in dendritic cells and not in other leukocytes, including monocyte precursors. TLR3 is the TLR that is expressed most strongly in the brain, especially in astrocytes, glia, and neurons.

    • Involvement in disease

      Defects in TLR3 are associated with herpes simplex encephalitis type 2 (HSE2) [MIM:613002]. HSE is a rare complication of human herpesvirus 1 (HHV-1) infection, occurring in only a small minority of HHV-1 infected individuals. HSE is characterized by hemorrhagic necrosis of parts of the temporal and frontal lobes. Onset is over several days and involves fever, headache, seizures, stupor, and often coma, frequently with a fatal outcome. Note=TLR3 mutations predispose otherwise healthy individuals to isolated herpes simplex encephalitis through a mechanism that involves impaired IFNs production and reduced immune defense against viral infection in the central nervous system.

    • Sequence similarities

      Belongs to the Toll-like receptor family.
      Contains 22 LRR (leucine-rich) repeats.
      Contains 1 LRRCT domain.
      Contains 1 LRRNT domain.
      Contains 1 TIR domain.

    • Domain

      ds-RNA binding is mediated by LRR 1 to 3, and LRR 17 to 18.

    • Post-translational
      modifications

      Heavily N-glycosylated, except on that part of the surface of the ectodomain that is involved in ligand binding.

    • Cellular localization

      Endoplasmic reticulum membrane. Endosome membrane.
    • Information by UniProt

    • Database links

      • Alternative names

        • CD283 antibody
        • CD283 antigen antibody
        • IIAE2 antibody
        • TLR 3 antibody
        • Tlr3 antibody
        • TLR3_HUMAN antibody
        • Toll Like Receptor 3 antibody
        • Toll-like receptor 3 antibody
        see all

      Images

      • Flow Cytometry - PE Anti-TLR3 antibody [40C1285.6] (ab45093)
        Flow Cytometry - PE Anti-TLR3 antibody [40C1285.6] (ab45093)This image is courtesy of an anonymous Abreview

        ab45093 staining TLR3 in Human A549 (lung epithelial) cell line by Flow Cytometry. The sample was incubated with the primary antibody (5μg/ml in 0.1% Saponin + 1% FCS in PBS) for 45 minutes at 4°C. 1 = isotype control, 2 = ab45093

         

        See Abreview

      • Flow Cytometry - PE Anti-TLR3 antibody [40C1285.6] (ab45093)
        Flow Cytometry - PE Anti-TLR3 antibody [40C1285.6] (ab45093)
        ab45093, at 3 ug/ 1X10^6 cells, staining Ramos cells by Intracellular flow cytometric analysis. Toll-Like-Receptor3 (open peak) and isotype negative control (shaded peak).

      References (1)

      Publishing research using ab45093? Please let us know so that we can cite the reference in this datasheet.

      ab45093 has been referenced in 1 publication.

      • Franzen-Röhl E  et al. Increased cell-mediated immune responses in patients with recurrent herpes simplex virus type 2 meningitis. Clin Vaccine Immunol 18:655-60 (2011). Flow Cyt . PubMed: 21325490

      Customer reviews and Q&As

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      1-2 of 2 Abreviews or Q&A

      Western blot abreview for Anti-TLR3 antibody [40C1285.6] (Phycoerythrin)

       Good
      Abreviews
      abreview image
      Application
      Western blot
      Loading amount
      15 µg
      Gel Running Conditions
      Reduced Non-Denaturing (Native) (5-16% gels)
      Sample
      Human Tissue lysate - whole (see below)
      Specification
      see below
      Blocking step
      BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
      Read More

      Abcam user community

      Verified customer

      Submitted Aug 01 2013

      Flow Cytometry abreview for Anti-TLR3 antibody [40C1285.6] (Phycoerythrin)

       Good
      Abreviews
      abreview image
      Application
      Flow Cytometry
      Fixation
      Paraformaldehyde
      Permeabilization
      Yes - 0,1% Saponin / PBS
      Sample
      Human Cell (A549 cells (lung epithelial cells))
      Specification
      A549 cells (lung epithelial cells)
      Gating Strategy
      no gate
      Preparation
      Cell harvesting/tissue preparation method: cell culture (commercially available cell line)
      Sample buffer: 0,1% Saponin /1% FCS/ PBS
      Read More

      Abcam user community

      Verified customer

      Submitted Jul 23 2013

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