PE Anti-TLR4/MD2 Complex antibody [MTS510] (ab95563)

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Flow Cytometry - PE Anti-TLR4/MD2 Complex antibody [MTS510] (ab95563)

    Key features and details

    • PE Rat monoclonal [MTS510] to TLR4/MD2 Complex
    • Suitable for: Flow Cyt
    • Reacts with: Mouse
    • Conjugation: PE. Ex: 488nm, Em: 575nm
    • Isotype: IgG2a

    Overview

    • Product name

      PE Anti-TLR4/MD2 Complex antibody [MTS510]
      See all TLR4/MD2 Complex primary antibodies

    • Description

      PE Rat monoclonal [MTS510] to TLR4/MD2 Complex

    • Host species

      Rat

    • Conjugation

      PE. Ex: 488nm, Em: 575nm

    • Specificity

      The MTS510 monoclonal antibody co-immunoprecipitates MD-2 (~30 kDa) and TLR4 (~100 kDa), and preferentially reacts with TLR4 that is associated with MD-2.

    • Tested applications

      Suitable for: Flow Cytmore details

    • Species reactivity

      Reacts with: Mouse

    • Immunogen

      Toll-like receptor 4 (TLR4)/MD-2 complex of mouse origin

    • Positive control

      • 3-day thioglycolate-induced BALB/c macrophages; mouse peritoneal exudate cells

    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    Associated products

    Applications

    Our Abpromise guarantee covers the use of ab95563 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Flow Cyt Use 0.5µg for 105-8 cells.

    Staining the cell sample in a final volume of 100 µL is recommended.

     

     

     

    ab134674 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

     

    Target

    • Function

      Cooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Also involved in LPS-independent inflammatory responses triggered by Ni(2+). These responses require non-conserved histidines and are, therefore, species-specific.

    • Tissue specificity

      Highly expressed in placenta, spleen and peripheral blood leukocytes. Detected in monocytes, macrophages, dendritic cells and several types of T-cells.

    • Involvement in disease

      Genetic variation in TLR4 is associated with age-related macular degeneration type 10 (ARMD10) [MIM:611488]. ARMD is a multifactorial eye disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid that lie beneath the retinal pigment epithelium and within an elastin-containing structure known as Bruch membrane.

    • Sequence similarities

      Belongs to the Toll-like receptor family.
      Contains 18 LRR (leucine-rich) repeats.
      Contains 1 LRRCT domain.
      Contains 1 TIR domain.

    • Domain

      The TIR domain mediates interaction with NOX4.

    • Post-translational
      modifications

      N-glycosylated. Glycosylation of Asn-526 and Asn-575 seems to be necessary for the expression of TLR4 on the cell surface and the LPS-response. Likewise, mutants lacking two or more of the other N-glycosylation sites were deficient in interaction with LPS.

    • Cellular localization

      Membrane.
    • Information by UniProt

    • Database links

      • Alternative names

        • ARMD10 antibody
        • CD284 antibody
        • CD284 antigen antibody
        • ESOP 1 antibody
        • ESOP1 antibody
        • Homolog of Drosophila toll antibody
        • hToll antibody
        • LY 96 antibody
        • LY96 antibody
        • Lymphocyte antigen 96 antibody
        • md 2 antibody
        • MD 2 protein antibody
        • MD2 protein antibody
        • Myeloid differentiation protein 2 antibody
        • Protein MD 2 antibody
        • Protein MD2 antibody
        • TLR 4 antibody
        • TLR4 antibody
        • TLR4_HUMAN antibody
        • TOLL antibody
        • Toll like receptor 4 antibody
        • Toll-like receptor 4 antibody
        see all

      Images

      • Flow Cytometry - PE Anti-TLR4/MD2 Complex antibody [MTS510] (ab95563)
        Flow Cytometry - PE Anti-TLR4/MD2 Complex antibody [MTS510] (ab95563)
        Staining of 3-day thioglycolate-induced BALB/c macrophages with 0.25 µg of Rat IgG2a ? Isotype Control PE (open histogram) or 0.25 µg of ab95563 (filled histogram). Cells were pre-incubated with Anti-Mouse CD16/CD32. Total viable cells were used for analysis.

      References (4)

      Publishing research using ab95563? Please let us know so that we can cite the reference in this datasheet.

      ab95563 has been referenced in 4 publications.

      • Chabot S  et al. TLRs regulate the gatekeeping functions of the intestinal follicle-associated epithelium. J Immunol 176:4275-83 (2006). PubMed: 16547265
      • Mortaz E  et al. Acetylsalicylic acid-induced release of HSP70 from mast cells results in cell activation through TLR pathway. Exp Hematol 34:8-18 (2006). PubMed: 16413386
      • Qi HY & Shelhamer JH Toll-like receptor 4 signaling regulates cytosolic phospholipase A2 activation and lipid generation in lipopolysaccharide-stimulated macrophages. J Biol Chem 280:38969-75 (2005). PubMed: 16176925
      • Ortega-Cava CF  et al. Strategic compartmentalization of Toll-like receptor 4 in the mouse gut. J Immunol 170:3977-85 (2003). PubMed: 12682225

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      Question

      Could you please help me decide which antibody would be the best for my planned experiments? I want to determine TLR4 expression on the cell surface of unstimulated human peripheral blood dendritic cells. Should I choose an anti TLR4 antibody (ab45126) or an anti TLR4/MD2 complex antibody (ab95563)? Could you explain me the different possibilities for those two antibodies?

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      Answer


      From the antibodies you have been looking at, I would suggest ab45126 would be most suitable depending on the application you hope to use the antibody in. The antibody ab95563 is directed against the mouse Toll-like receptor 4 (TLR4)/MD-2. The homology between the mouse and human proteins is not very high (67%), I would therefore not expect this antibody to be able to crossreact with the human protein.

      The Anti-TLR4 antibody [76B357.1] (FITC) (ab45126) has however been raised against the human TLR4 protein and has been used in ICC and flow cytometry. If this is how you intend to use the antibody this should be suitable for you. If you intend to use it in a different application you would be eligible to participate in our Abtrial scheme. More information on this scheme can be accessed from the following link:

      www.abcam.com/abtrial

      Read More

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