PE Anti-TLR8 antibody [44C143] (ab45097)
![Flow Cytometry (Intracellular) - PE Anti-TLR8 antibody [44C143] (ab45097)](https://www.abcam.com/ps/products/45/ab45097/Images/ab45097-57230-anti-tlr8-antibody-44c143-phycoerythrin-flow-cytometry.jpg "Flow Cytometry (Intracellular) - PE Anti-TLR8 antibody [44C143] (ab45097)")
Key features and details
- PE Mouse monoclonal [44C143] to TLR8
- Suitable for: Flow Cyt (Intra)
- Reacts with: Human
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Isotype: IgG1
Related conjugates and formulations
Overview
-
Product name
PE Anti-TLR8 antibody [44C143]
See all TLR8 primary antibodies -
Description
PE Mouse monoclonal [44C143] to TLR8 -
Host species
Mouse -
Conjugation
PE. Ex: 488nm, Em: 575nm -
Tested applications
Suitable for: Flow Cyt (Intra)more details -
Species reactivity
Reacts with: Human -
Immunogen
This antibody was developed against a KLH-conjugated synthetic peptide of human TLR8, within amino acids 750-850.
-
Positive control
- Flow Cyt (intra): Ramos or RAW cells.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
44C143 -
Isotype
IgG1 -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab45097 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
Use 2-5µg for 106 cells.
ab91357 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
| Notes |
|---|
|
Flow Cyt (Intra)
Use 2-5µg for 106 cells. ab91357 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Target
-
Function
Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific of microorganisms. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. -
Tissue specificity
Detected in brain, heart, lung, liver, placenta, in monocytes, and at lower levels in CD11c+ immature dendritic cells. -
Sequence similarities
Belongs to the Toll-like receptor family.
Contains 23 LRR (leucine-rich) repeats.
Contains 1 LRRCT domain.
Contains 1 TIR domain. -
Cellular localization
Membrane. - Information by UniProt
-
Database links
- Entrez Gene: 51311 Human
- Omim: 300366 Human
- SwissProt: Q9NR97 Human
- Unigene: 660543 Human
-
Alternative names
- CD 288 antibody
- CD288 antibody
- CD288 antigen antibody
see all
Images
-
Flow Cytometry (Intracellular) - PE Anti-TLR8 antibody [44C143] (ab45097)ab45097 at 2µg/106 cells staining Ramos cells by flow cytometry analysis. The shaded histogram represents cells without antibody, green represents isotype control and red represents ab45097.
Datasheets and documents
-
Datasheet download
References (5)
ab45097 has been referenced in 5 publications.
- Pritchard AL et al. Asthma is associated with multiple alterations in anti-viral innate signalling pathways. PLoS One 9:e106501 (2014). Flow Cyt ; Human . PubMed: 25203745
- Young BC et al. Longitudinal expression of Toll-like receptors on dendritic cells in uncomplicated pregnancy and postpartum. Am J Obstet Gynecol N/A:N/A (2013). Human . PubMed: 24291497
- Chong SZ et al. Human CD8? T cells drive Th1 responses through the differentiation of TNF/iNOS-producing dendritic cells. Eur J Immunol 41:1639-51 (2011). PubMed: 21469104
- Fahey LM et al. Reversal of human papillomavirus-specific T cell immune suppression through TLR agonist treatment of Langerhans cells exposed to human papillomavirus type 16. J Immunol 182:2919-28 (2009). Flow Cyt ; Human . PubMed: 19234187
- Levy O et al. Unique efficacy of Toll-like receptor 8 agonists in activating human neonatal antigen-presenting cells. Blood 108:1284-90 (2006). PubMed: 16638933
