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Validated using a knockout cell lineRecombinantRabMAb

Recombinant PE Anti-TMEM119 antibody [106-6] (ab225496)

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Flow Cytometry - PE Anti-TMEM119 antibody [106-6] (ab225496)
  • PE Anti-TMEM119 antibody [106-6] (ab225496)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • PE Rabbit monoclonal [106-6] to TMEM119
  • Suitable for: Flow Cyt
  • Knockout validated
  • Reacts with: Human
  • Conjugation: PE. Ex: 488nm, Em: 575nm

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 647 Carrier Free Unconjugated

Overview

  • Product name

    PE Anti-TMEM119 antibody [106-6]
    See all TMEM119 primary antibodies

  • Description

    PE Rabbit monoclonal [106-6] to TMEM119

  • Host species

    Rabbit

  • Conjugation

    PE. Ex: 488nm, Em: 575nm

  • Tested applications

    Suitable for: Flow Cytmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse

  • Immunogen

    Recombinant fragment (MBP-tag) within Mouse TMEM119 aa 1-100 (extracellular). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
    Database link: Q8R138

  • Positive control

    • Flow Cyt: HEK-293T transfected with Myc-His tagged TMEM119 expression vector

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab225496 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
1/500.

The cellular localisation of this product has been verified in ICC/IF.
Notes
Flow Cyt
1/500.

The cellular localisation of this product has been verified in ICC/IF.

Target

Images

  • Flow Cytometry - PE Anti-TMEM119 antibody [106-6] (ab225496)
    Flow Cytometry - PE Anti-TMEM119 antibody [106-6] (ab225496)

    Flow cytometry analysis of HEK-293T (human embryonic kidney) transfected with Myc-His tagged TMEM119 expression vector labelling TMEM119 with ab225496 at 1/500 dilution (right) compared with Rabbit IgG (monoclonal) Phycoerythrin ab209478 (left). Cells were surface-stained with ab225496, then fixed with 2% PFA for 10 minutes and permeabilised with 0.1% Tween-20 for 30 minutes. Next, they were stained with Alexa Fluor® 647 conjugated Myc-tag. Only Myc-tag (+) population showed TMEM119 positive staining.

  • PE Anti-TMEM119 antibody [106-6] (ab225496)
    PE Anti-TMEM119 antibody [106-6] (ab225496)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab225496? Please let us know so that we can cite the reference in this datasheet.

ab225496 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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