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Cell Biology Apoptosis Receptors Death Receptors
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RecombinantRabMAb

Recombinant PE Anti-TNF Receptor II antibody [EPR1653] (ab209582)

  • Datasheet
  • Certificate of Compliance
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Flow Cytometry - PE Anti-TNF Receptor II antibody [EPR1653] (ab209582)
  • PE Anti-TNF Receptor II antibody [EPR1653] (ab209582)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • PE Rabbit monoclonal [EPR1653] to TNF Receptor II
  • Suitable for: Flow Cyt
  • Reacts with: Human
  • Conjugation: PE. Ex: 488nm, Em: 575nm

You may also be interested in

Protein
Product image
Recombinant Human TNF Receptor II protein (ab206800)

View more associated products

Overview

  • Product name

    PE Anti-TNF Receptor II antibody [EPR1653]
    See all TNF Receptor II primary antibodies
  • Description

    PE Rabbit monoclonal [EPR1653] to TNF Receptor II
  • Host species

    Rabbit
  • Conjugation

    PE. Ex: 488nm, Em: 575nm
  • Tested applications

    Suitable for: Flow Cytmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human TNF Receptor II aa 400 to the C-terminus. The exact sequence is proprietary.
    Database link: P20333
    (Peptide available as ab192471)

  • Positive control

    • Flow Cyt: SW480 cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at +4°C. Do Not Freeze. Store In the Dark.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 1% BSA, PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR1653
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Apoptosis
    • Receptors
    • Death Receptors
    • Immunology
    • Innate Immunity
    • Cytokines
    • Necrosis Factors
    • Signal Transduction
    • Growth Factors/Hormones
    • TNF
    • Cancer
    • Growth factors
    • TNF
    • Cancer
    • Invasion/microenvironment
    • Angiogenesis
    • Angiogenic growth factors
    • Cardiovascular
    • Atherosclerosis
    • Ischemia / Reperfusion
    • Neuroscience
    • Neurology process
    • Neuroregeneration
    • Neuroregeneration

Associated products

  • Alternative Versions

    • Anti-TNF Receptor II antibody [EPR1653] (ab109322)
    • Alexa Fluor® 488 Anti-TNF Receptor II antibody [EPR1653] (ab199821)
    • Alexa Fluor® 647 Anti-TNF Receptor II antibody [EPR1653] (ab199822)
    • HRP Anti-TNF Receptor II antibody [EPR1653] (ab200006)
    • PerCP Anti-TNF Receptor II antibody [EPR1653] (ab220515)
    • Anti-TNF Receptor II antibody [EPR1653] - Low endotoxin, Azide free (ab221921)
    • Anti-TNF Receptor II antibody [EPR1653] - BSA and Azide free (ab226058)
  • Isotype control

    • PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab209478)
  • Recombinant Protein

    • Recombinant Human TNF Receptor II protein (ab206800)

Applications

Our Abpromise guarantee covers the use of ab209582 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/5000.

The cellular localisation of this product has been verified in ICC/IF.

Target

  • Function

    Receptor with high affinity for TNFSF2/TNF-alpha and approximately 5-fold lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha. The TRAF1/TRAF2 complex recruits the apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2. This receptor mediates most of the metabolic effects of TNF-alpha. Isoform 2 blocks TNF-alpha-induced apoptosis, which suggests that it regulates TNF-alpha function by antagonizing its biological activity.
  • Sequence similarities

    Contains 4 TNFR-Cys repeats.
  • Post-translational
    modifications

    Phosphorylated; mainly on serine residues and with a very low level on threonine residues.
    A soluble form (tumor necrosis factor binding protein 2) is produced from the membrane form by proteolytic processing.
  • Cellular localization

    Secreted and Cell membrane.
  • Target information above from: UniProt accession P20333 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 7133 Human
    • Entrez Gene: 21938 Mouse
    • Entrez Gene: 156767 Rat
    • Omim: 191191 Human
    • SwissProt: P20333 Human
    • SwissProt: P25119 Mouse
    • SwissProt: Q80WY6 Rat
    • Unigene: 256278 Human
    • Unigene: 235328 Mouse
    • Unigene: 83633 Rat
    see all
  • Alternative names

    • CD120b antibody
    • p75 antibody
    • p75 TNF receptor antibody
    • p75TNFR antibody
    • p80 TNF alpha receptor antibody
    • p80 TNF-alpha receptor antibody
    • Soluble TNFR1B variant 1 antibody
    • TBP-2 antibody
    • TBPII antibody
    • TNF R II antibody
    • TNF R2 antibody
    • TNF R75 antibody
    • TNF-R2 antibody
    • TNF-RII antibody
    • TNFBR antibody
    • TNFR-II antibody
    • TNFR1B antibody
    • TNFR2 antibody
    • TNFR80 antibody
    • TNFRII antibody
    • Tnfrsf1b antibody
    • TNR1B_HUMAN antibody
    • Tumor necrosis factor beta receptor antibody
    • Tumor necrosis factor binding protein 2 antibody
    • Tumor necrosis factor receptor 2 antibody
    • Tumor necrosis factor receptor superfamily member 1B antibody
    • Tumor necrosis factor receptor type II antibody
    • Tumor necrosis factor-binding protein 2 antibody
    see all

Images

  • Flow Cytometry - PE Anti-TNF Receptor II antibody [EPR1653] (ab209582)
    Flow Cytometry - PE Anti-TNF Receptor II antibody [EPR1653] (ab209582)
    Overlay histogram showing SW480 cells stained with ab209582 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 90% methanol at -20°C for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab209582, 1/5000 dilution) for 30 min at 22°C.

    Isotype control antibody (black line) was rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

    Acquisition of >5,000 events were collected using a 50 mW Yellow/Green laser (561nm) and 586/15 bandpass filter.
  • PE Anti-TNF Receptor II antibody [EPR1653] (ab209582)
    PE Anti-TNF Receptor II antibody [EPR1653] (ab209582)

Protocols

  • Flow cytometry protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

  • References (0)

    Publishing research using ab209582? Please let us know so that we can cite the reference in this datasheet.

    ab209582 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    There are currently no Customer reviews or Questions for ab209582.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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