RecombinantRabMAb

Recombinant PE Anti-TRAF6 antibody [EP591Y] (ab210412)

Submit a review Submit a question References (1)
Flow Cytometry (Intracellular) - PE Anti-TRAF6 antibody [EP591Y] (ab210412)
  • PE Anti-TRAF6 antibody [EP591Y] (ab210412)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • PE Rabbit monoclonal [EP591Y] to TRAF6
  • Suitable for: Flow Cyt (Intra)
  • Reacts with: Human
  • Conjugation: PE. Ex: 488nm, Em: 575nm

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 647 Carrier Free Unconjugated

Overview

  • Product name

    PE Anti-TRAF6 antibody [EP591Y]
    See all TRAF6 primary antibodies

  • Description

    PE Rabbit monoclonal [EP591Y] to TRAF6

  • Host species

    Rabbit

  • Conjugation

    PE. Ex: 488nm, Em: 575nm

  • Tested applications

    Suitable for: Flow Cyt (Intra)more details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Zebrafish

  • Immunogen

    Synthetic peptide within Human TRAF6 aa 50-150 (N terminal). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
    Database link: Q9Y4K3
    (Peptide available as ab183540)

  • Positive control

    • Flow Cyt (intra): HeLa cells

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab210412 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/5000.
Notes
Flow Cyt (Intra)
1/5000.

Target

  • Function

    E3 ubiquitin ligase that, together with UBE2N and UBE2V1, mediates the synthesis of 'Lys-63'-linked-polyubiquitin chains conjugated to proteins, such as IKBKG, AKT1 and AKT2. Also mediates ubiquitination of free/unanchored polyubiquitin chain that leads to MAP3K7 activation. Leads to the activation of NF-kappa-B and JUN. May be essential for the formation of functional osteoclasts. Seems to also play a role in dendritic cells (DCs) maturation and/or activation. Represses c-Myb-mediated transactivation, in B lymphocytes. Adapter protein that seems to play a role in signal transduction initiated via TNF receptor, IL-1 receptor and IL-17 receptor.

  • Tissue specificity

    Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.

  • Pathway

    Protein modification; protein ubiquitination.

  • Sequence similarities

    Belongs to the TNF receptor-associated factor family. A subfamily.
    Contains 1 MATH domain.
    Contains 1 RING-type zinc finger.
    Contains 2 TRAF-type zinc fingers.

  • Domain

    The coiled coil domain mediates homo- and hetero-oligomerization.
    The MATH/TRAF domain binds to receptor cytoplasmic domains.

  • Post-translational
    modifications

    Sumoylated on Lys-124, Lys-142 and Lys-453 by SUMO1.
    Polyubiquitinated on Lys-124; after cell stimulation with IL-1-beta or TGF-beta. This ligand-induced cell stimulation leads to dimerization/oligomerization of TRAF6 molecules, followed by auto-ubiquitination which involves UBE2N and UBE2V1 and leads to TRAF6 activation. This 'Lys-63' site-specific poly-ubiquitination appears to be associated with the activation of signaling molecules. Endogenous autoubiquitination occurs only for the cytoplasmic form.

  • Cellular localization

    Cytoplasm. Cytoplasm > cell cortex. Nucleus. Found in the nuclei of some agressive B-cell lymphoma cell lines as well as in the nuclei of both resting and activated T-and B-lymphocytes. Found in punctate nuclear body protein complexes. Ubiquitination may occur in the cytoplasm and sumoylation in the nucleus.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • E3 ubiquitin-protein ligase TRAF6 antibody
    • Interleukin 1 signal transducer antibody
    • Interleukin-1 signal transducer antibody
    • MGC 3310 antibody
    • MGC:3310 antibody
    • MGC3310 antibody
    • OTTHUMP00000232772 antibody
    • OTTHUMP00000232773 antibody
    • RING finger protein 85 antibody
    • RNF 85 antibody
    • RNF85 antibody
    • TNF receptor associated factor 6 antibody
    • TNF receptor-associated factor 6 antibody
    • TNF receptor-associated factor 6, E3 ubiquitin protein ligase antibody
    • TRAF 6 antibody
    • Traf6 antibody
    • TRAF6_HUMAN antibody
    see all

Images

  • Flow Cytometry (Intracellular) - PE Anti-TRAF6 antibody [EP591Y] (ab210412)
    Flow Cytometry (Intracellular) - PE Anti-TRAF6 antibody [EP591Y] (ab210412)

    Overlay histogram showing HeLa cells stained with ab210412 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 90% methanol (-20°C) for 30 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab210412, 1/5000 dilution) for 30 min at 22°C.

    Isotype control antibody (black line) was rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

    Acquisition of >5,000 events were collected using a 50mW Yellow-Green laser (561nm) and 586/15 bandpass filter.

  • PE Anti-TRAF6 antibody [EP591Y] (ab210412)
    PE Anti-TRAF6 antibody [EP591Y] (ab210412)

References (1)

Publishing research using ab210412? Please let us know so that we can cite the reference in this datasheet.

ab210412 has been referenced in 1 publication.

  • Garo LP  et al. MicroRNA-146a limits tumorigenic inflammation in colorectal cancer. Nat Commun 12:2419 (2021). PubMed: 33893298

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

There are currently no Customer reviews or Questions for ab210412.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com