Key features and details
- PE Rabbit polyclonal to V5 tag
- Suitable for: Flow Cyt, Sandwich ELISA
- Reacts with: Species independent
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Isotype: IgG
Product namePE Anti-V5 tag antibody
See all V5 tag primary antibodies
DescriptionPE Rabbit polyclonal to V5 tag
ConjugationPE. Ex: 488nm, Em: 575nm
Tested applicationsSuitable for: Flow Cyt, Sandwich ELISAmore details
Species reactivityReacts with: Species independent
Synthetic peptide V5 Tag - Tagged
Excitation max. lambda: 565>498 nm Emission max. lambda: 578 nm.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferpH: 7.40
Preservative: 0.013% Sodium azide
Constituents: 0.1% BSA, 0.87% Sodium chloride, 1.64% Sodium phosphate, 1.71% Sucrose
Concentration information loading...
Purification notesSize exclusion chromatography as well as some proprietary methods for purifying the final materials were used.
Our Abpromise guarantee covers the use of ab72480 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use at an assay dependent concentration.
ab37409 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
|Sandwich ELISA||Use at an assay dependent concentration.|
RelevanceThe V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD).
- GKPIPNPLLGLDST epitope tag antibody
- GKPIPNPLLGLDST tag antibody
- Protein Rev antibody
Flow cytometry analysis of a recombinant (N-terminus V5-tag) transmembrane cell surface receptor in CHO cells using ab72480 (red). Black - Mouse IgG1 Phycoerythrin isotype control.
Curve generated from a streptavidin-coated 96-well plate probed with dilutions of biotinylated V5 tagged peptide, and read after a 2 hour incubation at room temperature with ab72480.
ab72480 has been referenced in 1 publication.
- Dam Van P et al. Anti-Influenza A Virus Activities of Type I/III Interferons-Induced Mx1 GTPases from Different Mammalian Species. J Interferon Cytokine Res 39:274-282 (2019). PubMed: 30939061