Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- PE Rabbit monoclonal [EPR5047] to VCAM1
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: PE. Ex: 488nm, Em: 575nm
Product namePE Anti-VCAM1 antibody [EPR5047]
See all VCAM1 primary antibodies
DescriptionPE Rabbit monoclonal [EPR5047] to VCAM1
ConjugationPE. Ex: 488nm, Em: 575nm
Tested applicationsSuitable for: Flow Cytmore details
Species reactivityReacts with: Mouse
Predicted to work with: Rat, Human
- Flow Cyt: NIH3T3 cells
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at +4°C. Do Not Freeze. Store In the Dark.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, PBS
Concentration information loading...
PurityProtein A purified
- Anti-VCAM1 antibody [EPR5047] (ab134047)
- Alexa Fluor® 647 Anti-VCAM1 antibody [EPR5047] (ab194319)
- HRP Anti-VCAM1 antibody [EPR5047] (ab195540)
- Anti-VCAM1 antibody [EPR5047] - Low endotoxin, Azide free (ab215380)
- FITC Anti-VCAM1 antibody [EPR5047] (ab223982)
- APC Anti-VCAM1 antibody [EPR5047] (ab223983)
Our Abpromise guarantee covers the use of ab223981 in the following tested applications.
The cellular localisation of this product has been verified in ICC/IF.
FunctionImportant in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with the beta-1 integrin VLA4 on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/VLA4 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation.
Tissue specificityExpressed on inflammed vascular endothelium, as well as on macrophage-like and dendritic cell types in both normal and inflammed tissue.
Sequence similaritiesContains 7 Ig-like C2-type (immunoglobulin-like) domains.
DomainEither the first or the fourth Ig-like C2-type domain is required for VLA4-dependent cell adhesion.
- Information by UniProt
- CD106 antibody
- CD106 Antigen antibody
- INCAM 100 antibody
Overlay histogram showing NIH3T3 cells stained with ab223981 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab223981, 1/1000 dilution) for 30 min at 22°C.
Isotype control antibody (black line) was Rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 50 mW Yellow/Green laser (561nm) and 586/15 bandpass filter.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab223981 has not yet been referenced specifically in any publications.