PE Anti-Vimentin antibody [VI-RE/1] (ab49918)

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Immunocytochemistry/ Immunofluorescence - PE Anti-Vimentin antibody [VI-RE/1] (ab49918)
  • Flow Cytometry (Intracellular) - PE Anti-Vimentin antibody [VI-RE/1] (ab49918)

Key features and details

  • PE Mouse monoclonal [VI-RE/1] to Vimentin
  • Suitable for: ICC/IF, Flow Cyt (Intra)
  • Reacts with: Human
  • Conjugation: PE. Ex: 488nm, Em: 575nm
  • Isotype: IgG1

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PE Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab209446)
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Overview

  • Product name

    PE Anti-Vimentin antibody [VI-RE/1]
    See all Vimentin primary antibodies

  • Description

    PE Mouse monoclonal [VI-RE/1] to Vimentin

  • Host species

    Mouse

  • Conjugation

    PE. Ex: 488nm, Em: 575nm

  • Specificity

    The antibody VI-RE/1 reacts with human vimentin, a 57 kDa intermediate filament protein expressed on a wide variety of mesenchymal and mesodermal cell types.

  • Tested applications

    Suitable for: ICC/IF, Flow Cyt (Intra)more details

  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse, Pig

  • Immunogen

    Recombinant full length protein corresponding to Human Vimentin.
    Database link: P08670

  • Positive control

    • ICC/IF: HeLa cells Flow cyt: LEP-19 cells.

  • General notes

    Conjugated with R-Phycoerythrin (PE) under optimum conditions.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab49918 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
Use a concentration of 10 µg/ml.
Flow Cyt (Intra)
Use a concentration of 1 - 5 µg/ml.
Notes
ICC/IF
Use a concentration of 10 µg/ml.
Flow Cyt (Intra)
Use a concentration of 1 - 5 µg/ml.

Target

  • Function

    Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
    Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.

  • Tissue specificity

    Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines.

  • Involvement in disease

    Cataract 30

  • Sequence similarities

    Belongs to the intermediate filament family.

  • Domain

    The central alpha-helical coiled-coil rod region mediates elementary homodimerization.
    The [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex.

  • Post-translational
    modifications

    Filament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin (By similarity). One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylation by PKN1 inhibits the formation of filaments. Phosphorylated at Ser-56 by CDK5 during neutrophil secretion in the cytoplasm. Phosphorylated by STK33.
    O-glycosylated during cytokinesis at sites identical or close to phosphorylation sites, this interferes with the phosphorylation status.
    S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.

  • Cellular localization

    Cytoplasm.
  • Information by UniProt

  • Database links

    • Form

      Vimentin is found in connective tissue and in the cytoskeleton.

    • Alternative names

      • CTRCT30 antibody
      • Epididymis luminal protein 113 antibody
      • FLJ36605 antibody
      • HEL113 antibody
      • VIM antibody
      • VIME_HUMAN antibody
      • Vimentin antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - PE Anti-Vimentin antibody [VI-RE/1] (ab49918)
      Immunocytochemistry/ Immunofluorescence - PE Anti-Vimentin antibody [VI-RE/1] (ab49918)

      ab49918 staining Vimentinn in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized in 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab49918 at a working concentration of 10μg/ml (shown in red) and ab185031, Rabbit monoclonal [EP1332Y] to alpha Tubulin (Alexa Fluor® 488, shown in green) at 1/250 overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.
      Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    • Flow Cytometry (Intracellular) - PE Anti-Vimentin antibody [VI-RE/1] (ab49918)
      Flow Cytometry (Intracellular) - PE Anti-Vimentin antibody [VI-RE/1] (ab49918)

      Flow cytometry analysis (intracellular staining) of Vimentin  in LEP-19 human fibroblast cell line using ab49918 anti-human Vimentin (VI-RE/1) PE. Overlay with Isotype mouse IgG1 control. 

    References (5)

    Publishing research using ab49918? Please let us know so that we can cite the reference in this datasheet.

    ab49918 has been referenced in 5 publications.

    • Guo YX  et al. Diagnostic value of HE4+ circulating tumor cells in patients with suspicious ovarian cancer. Oncotarget 9:7522-7533 (2018). PubMed: 29484129
    • Cervelló I  et al. Reconstruction of endometrium from human endometrial side population cell lines. PLoS One 6:e21221 (2011). Flow Cyt ; Human . PubMed: 21712999
    • Basu D  et al. Detecting and targeting mesenchymal-like subpopulations within squamous cell carcinomas. Cell Cycle 10:2008-16 (2011). PubMed: 21558812
    • Basu D  et al. Evidence for mesenchymal-like sub-populations within squamous cell carcinomas possessing chemoresistance and phenotypic plasticity. Oncogene : (2010). Flow Cyt . PubMed: 20498638
    • Meyer MJ  et al. Dynamic regulation of CD24 and the invasive, CD44posCD24neg phenotype in breast cancer cell lines. Breast Cancer Res 11:R82 (2009). PubMed: 19906290

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