PE Anti-Vimentin antibody [VI-RE/1] (ab49918)

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Immunocytochemistry/ Immunofluorescence - PE Anti-Vimentin antibody [VI-RE/1] (ab49918)

    Key features and details

    • PE Mouse monoclonal [VI-RE/1] to Vimentin
    • Suitable for: ICC/IF
    • Reacts with: Human
    • Conjugation: PE. Ex: 488nm, Em: 575nm
    • Isotype: IgG1

    Overview

    • Product name

      PE Anti-Vimentin antibody [VI-RE/1]
      See all Vimentin primary antibodies

    • Description

      PE Mouse monoclonal [VI-RE/1] to Vimentin

    • Host species

      Mouse

    • Conjugation

      PE. Ex: 488nm, Em: 575nm

    • Specificity

      The antibody VI-RE/1 reacts with human vimentin, a 57 kDa intermediate filament protein expressed on a wide variety of mesenchymal and mesodermal cell types.

    • Tested applications

      Suitable for: ICC/IFmore details

    • Species reactivity

      Reacts with: Human
      Does not react with: Mouse, Pig

    • Immunogen

      Recombinant human vimentin.

    • Positive control

      • ICC/IF: HeLa cells

    • General notes

      Conjugated with R-Phycoerythrin (PE) under optimum conditions.

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    Applications

    Our Abpromise guarantee covers the use of ab49918 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    ICC/IF Use a concentration of 10 µg/ml.

    Target

    • Function

      Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
      Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.

    • Tissue specificity

      Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines.

    • Involvement in disease

      Cataract 30

    • Sequence similarities

      Belongs to the intermediate filament family.

    • Domain

      The central alpha-helical coiled-coil rod region mediates elementary homodimerization.
      The [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex.

    • Post-translational
      modifications

      Filament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin (By similarity). One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylation by PKN1 inhibits the formation of filaments. Phosphorylated at Ser-56 by CDK5 during neutrophil secretion in the cytoplasm. Phosphorylated by STK33.
      O-glycosylated during cytokinesis at sites identical or close to phosphorylation sites, this interferes with the phosphorylation status.
      S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.

    • Cellular localization

      Cytoplasm.
    • Information by UniProt

    • Database links

      • Form

        Vimentin is found in connective tissue and in the cytoskeleton.

      • Alternative names

        • CTRCT30 antibody
        • Epididymis luminal protein 113 antibody
        • FLJ36605 antibody
        • HEL113 antibody
        • VIM antibody
        • VIME_HUMAN antibody
        • Vimentin antibody
        see all

      Images

      • Immunocytochemistry/ Immunofluorescence - PE Anti-Vimentin antibody [VI-RE/1] (ab49918)
        Immunocytochemistry/ Immunofluorescence - PE Anti-Vimentin antibody [VI-RE/1] (ab49918)

        ab49918 staining Vimentinn in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized in 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab49918 at a working concentration of 10μg/ml (shown in red) and ab185031, Rabbit monoclonal [EP1332Y] to alpha Tubulin (Alexa Fluor® 488, shown in green) at 1/250 overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.
        Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

      References (5)

      Publishing research using ab49918? Please let us know so that we can cite the reference in this datasheet.

      ab49918 has been referenced in 5 publications.

      • Guo YX  et al. Diagnostic value of HE4+ circulating tumor cells in patients with suspicious ovarian cancer. Oncotarget 9:7522-7533 (2018). PubMed: 29484129
      • Cervelló I  et al. Reconstruction of endometrium from human endometrial side population cell lines. PLoS One 6:e21221 (2011). Flow Cyt ; Human . PubMed: 21712999
      • Basu D  et al. Detecting and targeting mesenchymal-like subpopulations within squamous cell carcinomas. Cell Cycle 10:2008-16 (2011). PubMed: 21558812
      • Basu D  et al. Evidence for mesenchymal-like sub-populations within squamous cell carcinomas possessing chemoresistance and phenotypic plasticity. Oncogene : (2010). Flow Cyt . PubMed: 20498638
      • Meyer MJ  et al. Dynamic regulation of CD24 and the invasive, CD44posCD24neg phenotype in breast cancer cell lines. Breast Cancer Res 11:R82 (2009). PubMed: 19906290

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