RecombinantRabMAb

Recombinant PE Anti-Wnt2b antibody [EPR13386] (ab211617)

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Immunocytochemistry/ Immunofluorescence - PE Anti-Wnt2b antibody [EPR13386] (ab211617)
  • Flow Cytometry - PE Anti-Wnt2b antibody [EPR13386] (ab211617)
  • PE Anti-Wnt2b antibody [EPR13386] (ab211617)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • PE Rabbit monoclonal [EPR13386] to Wnt2b
  • Suitable for: ICC/IF, Flow Cyt
  • Reacts with: Human
  • Conjugation: PE. Ex: 488nm, Em: 575nm

Overview

  • Product name

    PE Anti-Wnt2b antibody [EPR13386]
    See all Wnt2b primary antibodies

  • Description

    PE Rabbit monoclonal [EPR13386] to Wnt2b

  • Host species

    Rabbit

  • Conjugation

    PE. Ex: 488nm, Em: 575nm

  • Tested applications

    Suitable for: ICC/IF, Flow Cytmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Synthetic peptide within Human Wnt2b aa 250-350 (Cysteine residue). The exact sequence is proprietary.
    Database link: Q93097

  • Positive control

    • Flow Cytometry: SW480 cells ICC/IF: SW480 cells

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab211617 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100.

This product gave a positive signal in SW480 cells fixed with 4% formaldehyde (10 min), and 100% methanol (5 min).
Flow Cyt 1/1000.

Target

  • Function

    Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters. May be involved in normal development or differentiation as well as in carcinogenesis.

  • Tissue specificity

    Isoform 1 is expressed in adult heart, brain, placenta, lung, prostate, testis, ovary, small intestine and colon. In the adult brain, it is mainly found in the caudate nucleus, subthalamic nucleus and thalamus. Also detected in fetal brain, lung and kidney. Isoform 2 is expressed in fetal brain, fetal lung, fetal kidney, caudate nucleus, testis and cancer cell lines.

  • Sequence similarities

    Belongs to the Wnt family.

  • Cellular localization

    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • Protein Wnt-13 antibody
    • Protein Wnt-2b antibody
    • Wingless type MMTV integration site family, member 13 antibody
    • Wingless type MMTV integration site family, member 2B antibody
    • WNT13 antibody
    • Wnt2b antibody
    • WNT2B_HUMAN antibody
    • XWNT2 antibody
    • XWNT2, Xenopus, homolog of antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - PE Anti-Wnt2b antibody [EPR13386] (ab211617)
    Immunocytochemistry/ Immunofluorescence - PE Anti-Wnt2b antibody [EPR13386] (ab211617)

    ab211617 staining Wnt2b in SW480 cells. The cells were fixed with 4% formaldehyde, permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab211617 at 1/100 dilution (pseudocolored in green). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    This product also gave a positive signal under the same testing conditions in SW480 cells fixed with 100%methanol.

  • Flow Cytometry - PE Anti-Wnt2b antibody [EPR13386] (ab211617)
    Flow Cytometry - PE Anti-Wnt2b antibody [EPR13386] (ab211617)

    Overlay histogram showing SW480 cells stained with ab211617 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab211617, 1/1000 dilution) for 30 min at 22°C.

    Isotype control antibody (black line) was rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

    Acquisition of >5,000 events were collected using a 50mW Yellow/Green laser (561nm) and 586/15 bandpass filter.

    This antibody gave a positive signal in SW480 cells fixed with 4% formaldehyde (10 min), permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.

  • PE Anti-Wnt2b antibody [EPR13386] (ab211617)
    PE Anti-Wnt2b antibody [EPR13386] (ab211617)

References (0)

Publishing research using ab211617? Please let us know so that we can cite the reference in this datasheet.

ab211617 has not yet been referenced specifically in any publications.

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