Overview

  • Product name

    Anti-PEA15 (phospho S104) antibody
  • Description

    Rabbit polyclonal to PEA15 (phospho S104)
  • Host species

    Rabbit
  • Specificity

    ab138647 detects endogenous levels of PEA15 only when phosphorylated at S104
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: African green monkey
    Predicted to work with: Mouse, Rat, Human
  • Immunogen

    Synthesized phospho-peptide derived from the internal region of Human PEA15 around the phosphorylation site of S104.

  • Positive control

    • COS7 cell lysate

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 49% PBS, 50% Glycerol, 0.88% Sodium chloride
    Note: PBS is without Mg2+ and Ca2+
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab138647 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab138647 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 15 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Blocks Ras-mediated inhibition of integrin activation and modulates the ERK MAP kinase cascade. Inhibits RPS6KA3 activities by retaining it in the cytoplasm (By similarity). Inhibits both TNFRSF6- and TNFRSF1A-mediated CASP8 activity and apoptosis. Regulates glucose transport by controlling both the content of SLC2A1 glucose transporters on the plasma membrane and the insulin-dependent trafficking of SLC2A4 from the cell interior to the surface.
  • Tissue specificity

    Ubiquitously expressed. Most abundant in tissues such as heart, brain, muscle and adipose tissue which utilize glucose as an energy source. Lower expression in glucose-producing tissues. Higher levels of expression are found in tissues from individuals with type 2 diabetes than in controls.
  • Sequence similarities

    Contains 1 DED (death effector) domain.
  • Post-translational
    modifications

    Phosphorylated by protein kinase C and calcium-calmodulin-dependent protein kinase. These phosphorylation events are modulated by neurotransmitters or hormones.
  • Cellular localization

    Cytoplasm. Associated with microtubules.
  • Information by UniProt
  • Database links

  • Alternative names

    • 15 kDa phosphoprotein enriched in astrocytes antibody
    • Astrocytic phosphoprotein PEA 15 antibody
    • Astrocytic phosphoprotein PEA-15 antibody
    • Astrocytic phosphoprotein PEA15 antibody
    • HMAT 1 antibody
    • HMAT1 antibody
    • Homolog of mouse MAT 1 oncogene antibody
    • Homolog of mouse MAT1 oncogene antibody
    • HUMMAT 1H antibody
    • HUMMAT1H antibody
    • MAT 1 antibody
    • MAT 1H antibody
    • MAT1 antibody
    • MAT1H antibody
    • PEA 15 antibody
    • Pea15 antibody
    • PEA15 protein antibody
    • PEA15_HUMAN antibody
    • PED antibody
    • Phosphoprotein enriched in astrocytes 15 antibody
    • Phosphoprotein enriched in astrocytes 15kD antibody
    • Phosphoprotein enriched in diabetes antibody
    see all

Images

  • Anti-PEA15 (phospho S104) antibody (ab138647) at 1/500 dilution + COS7 cell lysate (from cells treated with TNF at 20ng/ml for 5 minutes) at 30 µg

    Predicted band size: 15 kDa

References

ab138647 has not yet been referenced specifically in any publications.

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There are currently no Customer reviews or Questions for ab138647.
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