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    pea3-antibody-1a2g3-ab70425.pdf

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Microbiology Interspecies Interaction Host Virus Interaction
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Anti-Pea3 antibody [1A2G3] (ab70425)

  • Datasheet
  • SDS
Reviews (2) Submit a question References (3)

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Western blot - Anti-Pea3 antibody [1A2G3] (ab70425)
  • Western blot - Anti-Pea3 antibody [1A2G3] (ab70425)
  • Flow Cytometry - Anti-Pea3 antibody [1A2G3] (ab70425)

Key features and details

  • Mouse monoclonal [1A2G3] to Pea3
  • Suitable for: Flow Cyt, WB
  • Reacts with: Human, Chinese hamster, Recombinant fragment
  • Isotype: IgG1

You may also be interested in

Secondary
Product image
Goat Anti-Mouse IgG H&L (HRP) (ab205719)

View more associated products

Overview

  • Product name

    Anti-Pea3 antibody [1A2G3]
    See all Pea3 primary antibodies
  • Description

    Mouse monoclonal [1A2G3] to Pea3
  • Host species

    Mouse
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    WB
    Human
    Recombinant fragment
    See all applications and species data
  • Immunogen

    Recombinant fragment corresponding to Human Pea3 aa 50-109.
    Sequence:

    SEDLFQDLSHFQETWLAEAQVPDSDEQFVPDFHSENLAFHSPTTRIKKEP QSPRTDPALS


    Database link: P43268
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • Truncated Trx-Pea3 recombinant protein and full length Pea3 hIgGFc transfected CHO-K1 cell lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purified from tissue culture supernatant.
  • Clonality

    Monoclonal
  • Clone number

    1A2G3
  • Isotype

    IgG1
  • Research areas

    • Microbiology
    • Interspecies Interaction
    • Host Virus Interaction
    • Neuroscience
    • Neurology process
    • Neurogenesis
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Ets

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
    • Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control (ab91353)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab70425 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
Flow Cyt
Human
WB
Human
Recombinant fragment
All applications
Mouse
Application Abreviews Notes
Flow Cyt
1/50 - 1/100.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

WB
1/500 - 1/2000. Detects a band of approximately 60 kDa (predicted molecular weight: 54 kDa).
Notes
Flow Cyt
1/50 - 1/100.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

WB
1/500 - 1/2000. Detects a band of approximately 60 kDa (predicted molecular weight: 54 kDa).

Target

  • Function

    Transcriptional activator that binds to the enhancer of the adenovirus E1A gene; the core-binding sequence is 5'[AC]GGA[AT]GT-3'.
  • Sequence similarities

    Belongs to the ETS family.
    Contains 1 ETS DNA-binding domain.
  • Post-translational
    modifications

    Sumoylated; enhanced upon ERK/MAP kinase pathway activation, it positively regulates the transcriptional activator capacity. Sumoylation at Lys-96 probably requires phosphorylation at Ser-101. Transiently polysumoylated and desumoylated by SENP1. Sumoylation is a prerequisite to polyubiquitination which in turn increases proteasomal-mediated degradation. Probably polyubiquitinated by RNF4 and deubiquitinated by USP2.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession P43268 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 2118 Human
    • Entrez Gene: 18612 Mouse
    • Omim: 600711 Human
    • SwissProt: P43268 Human
    • SwissProt: P28322 Mouse
    • Unigene: 434059 Human
    • Unigene: 5025 Mouse
    • Alternative names

      • Adenovirus E1A enhancer binding protein antibody
      • Adenovirus E1A enhancer-binding protein antibody
      • E1A F antibody
      • E1A-F antibody
      • ETS translocation variant 4 antibody
      • ets variant gene 4 (E1A enhancer binding protein E1AF) antibody
      • ETV4 antibody
      • ETV4_HUMAN antibody
      • PEA3 antibody
      • PEA3 protein antibody
      • PEAS3 antibody
      • Polyomavirus enhancer activator 3 antibody
      • Polyomavirus enhancer activator 3 homolog antibody
      • Protein PEA3 antibody
      see all

    Images

    • Western blot - Anti-Pea3 antibody [1A2G3] (ab70425)
      Western blot - Anti-Pea3 antibody [1A2G3] (ab70425)
      All lanes : Anti-Pea3 antibody [1A2G3] (ab70425) at 1/500 dilution

      Lane 1 : Truncated Trx-Pea3 recombinant protein
      Lane 2 : Full length Pea3 hIgGFc transfected CHO-K1 cell lysate

      Predicted band size: 54 kDa
      Observed band size: 24,60 kDa why is the actual band size different from the predicted?

    • Western blot - Anti-Pea3 antibody [1A2G3] (ab70425)
      Western blot - Anti-Pea3 antibody [1A2G3] (ab70425)
      Anti-Pea3 antibody [1A2G3] (ab70425) at 1/2000 dilution + Cell lysates prepared from K562 cells at 100 µg

      Secondary
      HRP-conjugated Goat polyclonal to mouse IgG1


      Predicted band size: 54 kDa

    • Flow Cytometry - Anti-Pea3 antibody [1A2G3] (ab70425)
      Flow Cytometry - Anti-Pea3 antibody [1A2G3] (ab70425)
      Overlay histogram showing K562 cells stained with ab70425 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab70425, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in K562 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

    Protocols

    • Flow cytometry protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (3)

    Publishing research using ab70425? Please let us know so that we can cite the reference in this datasheet.

    ab70425 has been referenced in 3 publications.

    • Singsuksawat E  et al. Increased ETV4 expression correlates with estrogen-enhanced proliferation and invasiveness of cholangiocarcinoma cells. Cancer Cell Int 18:25 (2018). PubMed: 29467595
    • Gysin S  et al. Analysis of mRNA profiles after MEK1/2 inhibition in human pancreatic cancer cell lines reveals pathways involved in drug sensitivity. Mol Cancer Res 10:1607-19 (2012). WB ; Human . PubMed: 22833572
    • Ahmad I  et al. K-Ras and ß-catenin mutations cooperate with Fgfr3 mutations in mice to promote tumorigenesis in the skin and lung, but not in the bladder. Dis Model Mech 4:548-55 (2011). IHC . PubMed: 21504907

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-2 of 2 Abreviews or Q&A

    Immunohistochemistry (Frozen sections) abreview for Anti-Pea3 antibody [1A2G3]

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (spinal cord)
    Permeabilization
    Yes - triton 0,2%
    Specification
    spinal cord
    Blocking step
    Serum as blocking agent for 15 minute(s) · Concentration: 10%
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted May 09 2017

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Pea3 antibody [1A2G3;4B11E5;7D2E5]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (human bladder transitional cell carcinoma)
    Specification
    human bladder transitional cell carcinoma
    Fixative
    Formaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate buffer
    Permeabilization
    No
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 5%
    Read More

    Dr. Imran Ahmad

    Verified customer

    Submitted Nov 29 2010

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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