Anti-Pea3 antibody [1A2G3] (ab70425)
![Western blot - Anti-Pea3 antibody [1A2G3] (ab70425)](https://www.abcam.com/ps/products/70/ab70425/Images/ab70425-85057-anti-pea3-antibody-1a2g3-western-blot.gif "Western blot - Anti-Pea3 antibody [1A2G3] (ab70425)")
Key features and details
- Mouse monoclonal [1A2G3] to Pea3
- Suitable for: Flow Cyt, WB
- Reacts with: Human, Recombinant fragment
- Isotype: IgG1
Overview
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Product name
Anti-Pea3 antibody [1A2G3]
See all Pea3 primary antibodies -
Description
Mouse monoclonal [1A2G3] to Pea3 -
Host species
Mouse -
Tested applications
Suitable for: Flow Cyt, WBmore details -
Species reactivity
Reacts with: Human, Recombinant fragment
Predicted to work with: Mouse
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Immunogen
Recombinant fragment corresponding to Human Pea3 aa 50-109.
Sequence:SEDLFQDLSHFQETWLAEAQVPDSDEQFVPDFHSENLAFHSPTTRIKKEP QSPRTDPALS
Database link: P43268 -
Positive control
- Truncated Trx-Pea3 recombinant protein and full length Pea3 hIgGFc transfected CHO-K1 cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
1A2G3 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab70425 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
1/50 - 1/100.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
|
| WB |
1/500 - 1/2000. Detects a band of approximately 60 kDa (predicted molecular weight: 54 kDa).
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| Notes |
|---|
|
Flow Cyt
1/50 - 1/100. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
|
WB
1/500 - 1/2000. Detects a band of approximately 60 kDa (predicted molecular weight: 54 kDa). |
Target
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Function
Transcriptional activator that binds to the enhancer of the adenovirus E1A gene; the core-binding sequence is 5'[AC]GGA[AT]GT-3'. -
Sequence similarities
Belongs to the ETS family.
Contains 1 ETS DNA-binding domain. -
Post-translational
modificationsSumoylated; enhanced upon ERK/MAP kinase pathway activation, it positively regulates the transcriptional activator capacity. Sumoylation at Lys-96 probably requires phosphorylation at Ser-101. Transiently polysumoylated and desumoylated by SENP1. Sumoylation is a prerequisite to polyubiquitination which in turn increases proteasomal-mediated degradation. Probably polyubiquitinated by RNF4 and deubiquitinated by USP2. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 2118 Human
- Entrez Gene: 18612 Mouse
- Omim: 600711 Human
- SwissProt: P43268 Human
- SwissProt: P28322 Mouse
- Unigene: 434059 Human
- Unigene: 5025 Mouse
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Alternative names
- Adenovirus E1A enhancer binding protein antibody
- Adenovirus E1A enhancer-binding protein antibody
- E1A F antibody
see all
Images
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Western blot - Anti-Pea3 antibody [1A2G3] (ab70425)All lanes : Anti-Pea3 antibody [1A2G3] (ab70425) at 1/500 dilution
Lane 1 : Truncated Trx-Pea3 recombinant protein
Lane 2 : Full length Pea3 hIgGFc transfected CHO-K1 cell lysate
Predicted band size: 54 kDa
Observed band size: 24,60 kDa why is the actual band size different from the predicted? -
![Western blot - Anti-Pea3 antibody [1A2G3] (ab70425)](https://www.abcam.com/ps/products/70/ab70425/Images/ab70425-85056-anti-pea3-antibody-1a2g3-western-blot.jpg)
Western blot - Anti-Pea3 antibody [1A2G3] (ab70425)Anti-Pea3 antibody [1A2G3] (ab70425) at 1/2000 dilution + Cell lysates prepared from K562 cells at 100 µg
Secondary
HRP-conjugated Goat polyclonal to mouse IgG1
Predicted band size: 54 kDa -
![Flow Cytometry - Anti-Pea3 antibody [1A2G3] (ab70425)](https://www.abcam.com/ps/products/70/ab70425/Images/ab70425-85054-anti-pea3-antibody-1a2g3-flow-cytometry.jpg)
Flow Cytometry - Anti-Pea3 antibody [1A2G3] (ab70425)Overlay histogram showing K562 cells stained with ab70425 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab70425, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in K562 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
Datasheets and documents
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SDS download
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Datasheet download
References (7)
ab70425 has been referenced in 7 publications.
- Leng Y et al. Overexpression of microRNA-29b inhibits epithelial-mesenchymal transition and angiogenesis of colorectal cancer through the ETV4/ERK/EGFR axis. Cancer Cell Int 21:17 (2021). PubMed: 33407520
- Yu W et al. Transcription activation of microRNA-25 by PEA3 augments progression of gastric cancer through suppressing SIK1. Exp Physiol N/A:N/A (2021). PubMed: 33876502
- Liu Y et al. Discovery of key genes as novel biomarkers specifically associated with HPV-negative cervical cancer. Mol Ther Methods Clin Dev 21:492-506 (2021). PubMed: 33997099
- Wu X et al. Icaritin reduces prostate cancer progression via inhibiting high-fat diet-induced serum adipokine in TRAMP mice model. J Cancer 11:6556-6564 (2020). PubMed: 33046976
- Singsuksawat E et al. Increased ETV4 expression correlates with estrogen-enhanced proliferation and invasiveness of cholangiocarcinoma cells. Cancer Cell Int 18:25 (2018). PubMed: 29467595
- Gysin S et al. Analysis of mRNA profiles after MEK1/2 inhibition in human pancreatic cancer cell lines reveals pathways involved in drug sensitivity. Mol Cancer Res 10:1607-19 (2012). WB ; Human . PubMed: 22833572
- Ahmad I et al. K-Ras and ß-catenin mutations cooperate with Fgfr3 mutations in mice to promote tumorigenesis in the skin and lung, but not in the bladder. Dis Model Mech 4:548-55 (2011). IHC . PubMed: 21504907