Product nameAnti-Pea3 antibody
See all Pea3 primary antibodies
DescriptionRabbit polyclonal to Pea3
Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
Recombinant fragment corresponding to Human Pea3.
Database link: P43268
- SW480, H460, SKOV3, BT474, Raji, Mouse skeletal muscle and mouse heart cell extracts.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab189826 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|WB||1/500 - 1/2000. Predicted molecular weight: 54 kDa.|
|IHC-P||1/50 - 1/200.|
FunctionTranscriptional activator that binds to the enhancer of the adenovirus E1A gene; the core-binding sequence is 5'[AC]GGA[AT]GT-3'.
Sequence similaritiesBelongs to the ETS family.
Contains 1 ETS DNA-binding domain.
modificationsSumoylated; enhanced upon ERK/MAP kinase pathway activation, it positively regulates the transcriptional activator capacity. Sumoylation at Lys-96 probably requires phosphorylation at Ser-101. Transiently polysumoylated and desumoylated by SENP1. Sumoylation is a prerequisite to polyubiquitination which in turn increases proteasomal-mediated degradation. Probably polyubiquitinated by RNF4 and deubiquitinated by USP2.
- Information by UniProt
- Adenovirus E1A enhancer binding protein antibody
- Adenovirus E1A enhancer-binding protein antibody
- E1A F antibody
Immunocytochemistry/Immunofluorescence analysis of U2OS cells using ab189826. Blue DAPI for nuclear staining.
All lanes : Anti-Pea3 antibody (ab189826) at 1/500 dilution
Lane 1 : SW480 cell extract
Lane 2 : H460 cell extract
Lane 3 : SKOV3 cell extract
Lane 4 : BT474 cell extract
Lane 5 : Raji cell extract
Lane 6 : Mouse skeletal muscle cell extract
Lane 7 : Mouse heart cell extract
Developed using the ECL technique.
Predicted band size: 54 kDa
ab189826 has not yet been referenced specifically in any publications.