Overview

  • Product name

  • Description

    Rabbit polyclonal to Pea3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment corresponding to Human Pea3.
    Database link: P43268

  • Positive control

    • SW480, H460, SKOV3, BT474, Raji, Mouse skeletal muscle and mouse heart cell extracts.

Properties

Applications

Our Abpromise guarantee covers the use of ab189826 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
WB 1/500 - 1/2000. Predicted molecular weight: 54 kDa.
IHC-P 1/50 - 1/200.

Target

  • Function

    Transcriptional activator that binds to the enhancer of the adenovirus E1A gene; the core-binding sequence is 5'[AC]GGA[AT]GT-3'.
  • Sequence similarities

    Belongs to the ETS family.
    Contains 1 ETS DNA-binding domain.
  • Post-translational
    modifications

    Sumoylated; enhanced upon ERK/MAP kinase pathway activation, it positively regulates the transcriptional activator capacity. Sumoylation at Lys-96 probably requires phosphorylation at Ser-101. Transiently polysumoylated and desumoylated by SENP1. Sumoylation is a prerequisite to polyubiquitination which in turn increases proteasomal-mediated degradation. Probably polyubiquitinated by RNF4 and deubiquitinated by USP2.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Adenovirus E1A enhancer binding protein antibody
    • Adenovirus E1A enhancer-binding protein antibody
    • E1A F antibody
    • E1A-F antibody
    • ETS translocation variant 4 antibody
    • ets variant gene 4 (E1A enhancer binding protein E1AF) antibody
    • ETV4 antibody
    • ETV4_HUMAN antibody
    • PEA3 antibody
    • PEA3 protein antibody
    • PEAS3 antibody
    • Polyomavirus enhancer activator 3 antibody
    • Polyomavirus enhancer activator 3 homolog antibody
    • Protein PEA3 antibody
    see all

Images

  • Immunocytochemistry/Immunofluorescence analysis of U2OS cells using ab189826. Blue DAPI for nuclear staining.
  • All lanes : Anti-Pea3 antibody (ab189826) at 1/500 dilution

    Lane 1 : SW480 cell extract
    Lane 2 : H460 cell extract
    Lane 3 : SKOV3 cell extract
    Lane 4 : BT474 cell extract
    Lane 5 : Raji cell extract
    Lane 6 : Mouse skeletal muscle cell extract
    Lane 7 : Mouse heart cell extract

    Developed using the ECL technique.

    Predicted band size: 54 kDa

References

ab189826 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Application
Immunohistochemistry (Frozen sections)
Sample
Chicken Tissue sections (Embryonic spinal cord)
Permeabilization
Yes - 0.1%Triton X-100
Specification
Embryonic spinal cord
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 20°C
Fixative
Paraformaldehyde

Dr. Frederic Clotman

Verified customer

Submitted Jul 07 2017

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (embryo E12 spinal cord)
Permeabilization
Yes - triton 0,2%
Specification
embryo E12 spinal cord
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 20°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted May 23 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Zebrafish Tissue sections (whole fish larvae)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer pH 6.0
Permeabilization
No
Specification
whole fish larvae
Blocking step
fetal bovine serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 28°C
Fixative
Paraformaldehyde

Dr. Enrico Moro

Verified customer

Submitted Jul 20 2015

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