Overview

  • Product name

    Anti-Pea3 antibody [OTI5C11]
    See all Pea3 primary antibodies
  • Description

    Mouse monoclonal [OTI5C11] to Pea3
  • Host species

    Mouse
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human Pea3 aa 1-276. (NP_001073143) produced in E.coli.
    Database link: P43268

  • Positive control

    • WB: HEK-293T transfected with pCMV6-ENTRY Pea3 whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab236503 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Predicted molecular weight: 54 kDa.

Target

  • Function

    Transcriptional activator that binds to the enhancer of the adenovirus E1A gene; the core-binding sequence is 5'[AC]GGA[AT]GT-3'.
  • Sequence similarities

    Belongs to the ETS family.
    Contains 1 ETS DNA-binding domain.
  • Post-translational
    modifications

    Sumoylated; enhanced upon ERK/MAP kinase pathway activation, it positively regulates the transcriptional activator capacity. Sumoylation at Lys-96 probably requires phosphorylation at Ser-101. Transiently polysumoylated and desumoylated by SENP1. Sumoylation is a prerequisite to polyubiquitination which in turn increases proteasomal-mediated degradation. Probably polyubiquitinated by RNF4 and deubiquitinated by USP2.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Adenovirus E1A enhancer binding protein antibody
    • Adenovirus E1A enhancer-binding protein antibody
    • E1A F antibody
    • E1A-F antibody
    • ETS translocation variant 4 antibody
    • ets variant gene 4 (E1A enhancer binding protein E1AF) antibody
    • ETV4 antibody
    • ETV4_HUMAN antibody
    • PEA3 antibody
    • PEA3 protein antibody
    • PEAS3 antibody
    • Polyomavirus enhancer activator 3 antibody
    • Polyomavirus enhancer activator 3 homolog antibody
    • Protein PEA3 antibody
    see all

Images

  • All lanes : Anti-Pea3 antibody [OTI5C11] (ab236503) at 1/2000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with pCMV6-ENTRY control, cell lysate
    Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with pCMV6-ENTRY Pea3, cell lysate

    Lysates/proteins at 5 µg per lane.

    Predicted band size: 54 kDa

References

ab236503 has not yet been referenced specifically in any publications.

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