Anti-PEAMT antibody (ab237686)
Key features and details
- Rabbit polyclonal to PEAMT
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PEAMT antibody
See all PEAMT primary antibodies -
Description
Rabbit polyclonal to PEAMT -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human PEAMT aa 2-49.
Database link: Q9UBM1 -
Positive control
- WB: SH-SY5Y whole cell lysate. ICC/IF: A549 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purity greater than 95%. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab237686 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC/IF |
1/50 - 1/200.
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|
WB |
1/1000 - 1/5000.
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Notes |
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ICC/IF
1/50 - 1/200. |
WB
1/1000 - 1/5000. |
Target
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Function
Catalyzes three sequential methylation of phosphatidylethanolamine (PE) by AdoMet, thus producing phosphatidylcholine (PC). -
Sequence similarities
Belongs to the PEMT/PEM2 methyltransferase family. -
Cellular localization
Endoplasmic reticulum membrane. Mitochondrion membrane. Found in endoplasmic reticulum where most PEMT activity is generated and in mitochondria. - Information by UniProt
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Database links
- Entrez Gene: 10400 Human
- Omim: 602391 Human
- SwissProt: Q9UBM1 Human
- Unigene: 287717 Human
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Alternative names
- PEAMT antibody
- PEMPT antibody
- PEMT antibody
see all
Images
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Anti-PEAMT antibody (ab237686) at 1/1000 dilution + SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution -
A549 (Human lung carcinoma cell line) cells stained for PEAMT (green) using ab237686 at 1/100 dilution in ICC/IF, followed by an Alexa-Fluor®488-conjugated Goat Anti-Rabbit IgG (H+L) secondary antibody.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab237686 has not yet been referenced specifically in any publications.