Key features and details
- Rabbit polyclonal to PEAMT
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-PEAMT antibody
See all PEAMT primary antibodies
DescriptionRabbit polyclonal to PEAMT
Tested applicationsSuitable for: ICC/IF, WBmore details
Species reactivityReacts with: Human
Recombinant fragment corresponding to Human PEAMT aa 2-49.
Database link: Q9UBM1
- WB: SH-SY5Y whole cell lysate. ICC/IF: A549 cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol, PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity greater than 95%.
Our Abpromise guarantee covers the use of ab237686 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/50 - 1/200.|
|WB||1/1000 - 1/5000.|
FunctionCatalyzes three sequential methylation of phosphatidylethanolamine (PE) by AdoMet, thus producing phosphatidylcholine (PC).
Sequence similaritiesBelongs to the PEMT/PEM2 methyltransferase family.
Cellular localizationEndoplasmic reticulum membrane. Mitochondrion membrane. Found in endoplasmic reticulum where most PEMT activity is generated and in mitochondria.
- Information by UniProt
- PEAMT antibody
- PEMPT antibody
- PEMT antibody
Anti-PEAMT antibody (ab237686) at 1/1000 dilution + SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate
Goat polyclonal to rabbit IgG at 1/50000 dilution
A549 (Human lung carcinoma cell line) cells stained for PEAMT (green) using ab237686 at 1/100 dilution in ICC/IF, followed by an Alexa-Fluor®488-conjugated Goat Anti-Rabbit IgG (H+L) secondary antibody.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab237686 has not yet been referenced specifically in any publications.