Recombinant
RabMAb

Anti-PEAMT antibody [EPR6919] (ab126610)

Overview

  • Product name
    Anti-PEAMT antibody [EPR6919]
    See all PEAMT primary antibodies
  • Description
    Rabbit monoclonal [EPR6919] to PEAMT
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human PEAMT aa 150-250 (C terminal). The exact sequence is proprietary.

  • Positive control
    • MOLT4, SK OV 3, HepG2 and MCF7 lysates; Human papillary adenocarcinoma and uterus tissues.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab126610 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 19 kDa (predicted molecular weight: 22 kDa).
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IP.
  • Target

    • Function
      Catalyzes three sequential methylation of phosphatidylethanolamine (PE) by AdoMet, thus producing phosphatidylcholine (PC).
    • Sequence similarities
      Belongs to the PEMT/PEM2 methyltransferase family.
    • Cellular localization
      Endoplasmic reticulum membrane. Mitochondrion membrane. Found in endoplasmic reticulum where most PEMT activity is generated and in mitochondria.
    • Information by UniProt
    • Database links
    • Alternative names
      • PEAMT antibody
      • PEMPT antibody
      • PEMT antibody
      • PEMT_HUMAN antibody
      • PEMT2 antibody
      • phosphatidylethanolamine N methyltransferase antibody
      • Phosphatidylethanolamine N-methyltransferase antibody
      • PNMT antibody
      see all

    Images

    • All lanes : Anti-PEAMT antibody [EPR6919] (ab126610) at 1/1000 dilution

      Lane 1 : MOLT4 whole cell lysate
      Lane 2 : SK OV 3 whole cell lysate
      Lane 3 : HepG2 whole cell lysate
      Lane 4 : MCF7 whole cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 22 kDa

    • ab126610, at 1/50 dilution, staining PEAMT in Formalin-fixed, Paraffin-embedded Human papillary adenocarcinoma tissue by Immunohistochemistry.
    • ab126610, at 1/50 dilution, staining PEAMT in Formalin-fixed, Paraffin-embedded Human uterus tissue by Immunohistochemistry.
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    ab126610 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue sections (glioblastoma cryosection)
    Permeabilization
    No
    Specification
    glioblastoma cryosection
    Blocking step
    BSA as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Username

    Mrs. avigail zucker

    Verified customer

    Submitted Apr 26 2018

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up