Anti-PEAR1 antibody (ab122261)

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Immunocytochemistry/ Immunofluorescence - Anti-PEAR1 antibody (ab122261)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PEAR1 antibody (ab122261)

Key features and details

  • Rabbit polyclonal to PEAR1
  • Suitable for: ICC/IF, IHC-P
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-PEAR1 antibody

  • Description

    Rabbit polyclonal to PEAR1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, IHC-Pmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    antigen sequence: PSDPNTCSFW ESFTTTTKES HSRPFSLLPS EPCERPWEGP HTCPQPTVVY RTVYRQVVKT DHRQRLQCCH GFYES, corresponding to amino acids 23-97 of Human.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • IHC: Human bone marrow tissue. ICC/IF: Human cell line U-2 OS.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.

  • Storage buffer

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab122261 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
Use a concentration of 0.25 - 2 µg/ml.
IHC-P
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
ICC/IF
Use a concentration of 0.25 - 2 µg/ml.
IHC-P
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    When overexpressed, reduces the number of both early and late non-adherent myeloid progenitor cells.

  • Tissue specificity

    Expressed in umbilical vein endothelial cells and platelets (at protein level). Expressed in heart, kidney, skeletal muscle, pancreas, ovary, breast, lung, brain cortex, hypothalamus, spinal cord, dorsal root ganglion, endothelial cells of umbilical cord artery and vein, megakaryocytes, osteoblasts, coronary muscle and erythroid cells. Weakly expressed in peripheral blood leukocytes and macrophages.

  • Sequence similarities

    Belongs to the MEGF family.
    Contains 9 EGF-like domains.
    Contains 1 EMI domain.

  • Post-translational
    modifications

    Phosphorylated in the intracellular domain on tyrosine residues (By similarity). Phosphorylated on tyrosine residues by SRC. Tyrosine phosphorylation is detected upon platelet aggregation stimulated by collagen, TRAP and thrombin and platelet-platelet contacts but not after platelet activation. Tyrosine phosphorylation enhanced its association with SHC1 and SHC2.

  • Cellular localization

    Cell membrane. Detected on the cell surface in resting platelets.
  • Information by UniProt

  • Database links

    • Alternative names

      • hPEAR1 antibody
      • JEDI antibody
      • MEGF12 antibody
      • Multiple EGF-like domains protein 12 antibody
      • Multiple epidermal growth factor-like domains protein 12 antibody
      • Pear1 antibody
      • PEAR1_HUMAN antibody
      • Platelet endothelial aggregation receptor 1 antibody
      • RP11-107D16.3 antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-PEAR1 antibody (ab122261)
      Immunocytochemistry/ Immunofluorescence - Anti-PEAR1 antibody (ab122261)

      Immunocytochemistry/immunofluorescence analysis of human cell line U-2 OS labelling PEAR1 with ab122261 at 2 µg/mL shows localization to centrosome.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PEAR1 antibody (ab122261)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PEAR1 antibody (ab122261)
      ab122261, at 1/35, staining PEAR1 in paraffin embedded Human bone marrow tissue by Immunohistochemistry.

    References (0)

    Publishing research using ab122261? Please let us know so that we can cite the reference in this datasheet.

    ab122261 has not yet been referenced specifically in any publications.

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