- Datasheet
- Specific References
- Protocols
Overview
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Product nameAnti-PEAR1 antibody
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DescriptionRabbit polyclonal to PEAR1
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Host speciesRabbit
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Tested applicationsSuitable for: IHC-Pmore details
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Species reactivityReacts with: Human
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Immunogen
antigen sequence: PSDPNTCSFW ESFTTTTKES HSRPFSLLPS EPCERPWEGP HTCPQPTVVY RTVYRQVVKT DHRQRLQCCH GFYES, corresponding to amino acids 23-97 of Human.
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Positive control
- Human bone marrow tissue.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
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Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab122261 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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FunctionWhen overexpressed, reduces the number of both early and late non-adherent myeloid progenitor cells.
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Tissue specificityExpressed in umbilical vein endothelial cells and platelets (at protein level). Expressed in heart, kidney, skeletal muscle, pancreas, ovary, breast, lung, brain cortex, hypothalamus, spinal cord, dorsal root ganglion, endothelial cells of umbilical cord artery and vein, megakaryocytes, osteoblasts, coronary muscle and erythroid cells. Weakly expressed in peripheral blood leukocytes and macrophages.
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Sequence similaritiesBelongs to the MEGF family.
Contains 9 EGF-like domains.
Contains 1 EMI domain. -
Post-translational
modificationsPhosphorylated in the intracellular domain on tyrosine residues (By similarity). Phosphorylated on tyrosine residues by SRC. Tyrosine phosphorylation is detected upon platelet aggregation stimulated by collagen, TRAP and thrombin and platelet-platelet contacts but not after platelet activation. Tyrosine phosphorylation enhanced its association with SHC1 and SHC2. -
Cellular localizationCell membrane. Detected on the cell surface in resting platelets.
- Information by UniProt
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Database links
- Entrez Gene: 375033 Human
- Omim: 610278 Human
- SwissProt: Q5VY43 Human
- Unigene: 142003 Human
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Alternative names
- hPEAR1 antibody
- JEDI antibody
- MEGF12 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PEAR1 antibody (ab122261)ab122261, at 1/35, staining PEAR1 in paraffin embedded Human bone marrow tissue by Immunohistochemistry.
Protocols
References
ab122261 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"