Anti-PEAR1 antibody (ab122261)
Key features and details
- Rabbit polyclonal to PEAR1
- Suitable for: ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PEAR1 antibody -
Description
Rabbit polyclonal to PEAR1 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
antigen sequence: PSDPNTCSFW ESFTTTTKES HSRPFSLLPS EPCERPWEGP HTCPQPTVVY RTVYRQVVKT DHRQRLQCCH GFYES, corresponding to amino acids 23-97 of Human.
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Positive control
- IHC: Human bone marrow tissue. ICC/IF: Human cell line U-2 OS.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab122261 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
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IHC-P |
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
IHC-P
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
When overexpressed, reduces the number of both early and late non-adherent myeloid progenitor cells. -
Tissue specificity
Expressed in umbilical vein endothelial cells and platelets (at protein level). Expressed in heart, kidney, skeletal muscle, pancreas, ovary, breast, lung, brain cortex, hypothalamus, spinal cord, dorsal root ganglion, endothelial cells of umbilical cord artery and vein, megakaryocytes, osteoblasts, coronary muscle and erythroid cells. Weakly expressed in peripheral blood leukocytes and macrophages. -
Sequence similarities
Belongs to the MEGF family.
Contains 9 EGF-like domains.
Contains 1 EMI domain. -
Post-translational
modificationsPhosphorylated in the intracellular domain on tyrosine residues (By similarity). Phosphorylated on tyrosine residues by SRC. Tyrosine phosphorylation is detected upon platelet aggregation stimulated by collagen, TRAP and thrombin and platelet-platelet contacts but not after platelet activation. Tyrosine phosphorylation enhanced its association with SHC1 and SHC2. -
Cellular localization
Cell membrane. Detected on the cell surface in resting platelets. - Information by UniProt
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Database links
- Entrez Gene: 375033 Human
- Omim: 610278 Human
- SwissProt: Q5VY43 Human
- Unigene: 142003 Human
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Alternative names
- hPEAR1 antibody
- JEDI antibody
- MEGF12 antibody
see all
Images
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Immunocytochemistry/immunofluorescence analysis of human cell line U-2 OS labelling PEAR1 with ab122261 at 2 µg/mL shows localization to centrosome.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PEAR1 antibody (ab122261)ab122261, at 1/35, staining PEAR1 in paraffin embedded Human bone marrow tissue by Immunohistochemistry.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab122261 has not yet been referenced specifically in any publications.