Product namePE/Atto 594 Conjugation Kit - Lightning-Link®
PE/Atto 594 Conjugation Kit / PE/Atto 594 Labeling Kit (ab269900) uses a simple and quick process for PE/Atto 594 labeling / conjugation of antibodies. It can also be used to conjugate other proteins or peptides. Learn about our antibody labeling kits and their advantages.
To conjugate an antibody to PE/Atto 594 using this kit:
- add modifier to antibody and incubate for 15 mins
- add quencher and incubate for 5 mins
The PE/Atto 594 conjugated antibody can be used immediately in WB, ELISA, IHC etc. No further purification is required and 100% of the antibody is recovered for use.
Learn about buffer compatibility below; for incompatible buffers and low antibody concentrations, use our rapid antibody purification and concentration kits. Use the FAQ to learn more about the technology, or about conjugating other proteins and peptides to PE/Atto 594.
Custom size conjugation kits up to 100 mg are available on demand. Please contact us to discuss your requirements.
This product is manufactured by Expedeon, an Abcam company, and was previously called Lightning-Link® R-PE/Atto 594 Labeling Kit. 768-0015 is the same as the 600 µg size. 768-0010 is the same as the 3 x 60 ug size. 768-0030 is the same as the 3 x 10 ug size. 768-0005 is the same as the 60 µg size.
Amount and volume of antibody for conjugation to PE/Atto 594
Kit size Recommended
amount of antibody1
amount of antibody
3 x 10 µg 3 x 10 µg 3 x 10 µg 3 x 10 µL 60 µg 60 µg 60 µg 60 µL 3 x 60 µg 3 x 60 µg 3 x 60 µg 3 x 60 µL 600 µg 0.6 mg
1 Using the maximum amount of antibody may result in less labeling per antibody.
2 Ideal antibody concentration is 1mg/ml. 0.5 - 1 mg/ml can be used if the maximum antibody volume is not exceeded. Antibodies > 1 mg/ml or < 0.5 mg/ml should be diluted /concentrated.
Buffer Requirements for Conjugation
Buffer should be pH 6.5-8.5.
Compatible buffer constituents
If a concentration is shown, then the constituent should be no more than the concentration shown. If several constituents are close to the limit of acceptable concentration, then this can inhibit conjugation.
50mM / 0.6% Tris1 0.1% BSA2 50% glycerol 0.1% sodium azide PBS Potassium phosphate Sodium chloride HEPES Sucrose Sodium citrate EDTA Trehalose
1 Tris buffered saline is almost always ≤ 50 mM / 0.6%
2 BSA can also interfere with the use of the conjugated antibody in tissue staining.
Incompatible buffer constituents
Thiomerosal Proclin Glycine Arginine Glutathione DTT
Only purified antibodies are suitable for use, ie. where other proteins, peptides, or amino acids are not present: antibodies in ascites fluid, serum or hybridoma culture media are incompatible.
Storage instructionsStore at -20°C. Please refer to protocols.
Components 60 µg 600 µg 3 x 10 µg 3 x 60 µg ab274106 - Modifier reagent 1 x 200µl 1 x 200µl 1 x 200µl 1 x 200µl ab274165 - PE/Atto 594 mix (Lyophilized) 1 x 60µg 1 x 600µg 3 x 10µg 3 x 60µg ab274133 - Quencher reagent 1 x 200µl 1 x 200µl 1 x 200µl 1 x 200µl
ab269900 has not yet been referenced specifically in any publications.