Key features and details
- Rabbit polyclonal to PECI/ECI2
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-PECI/ECI2 antibody
See all PECI/ECI2 primary antibodies
DescriptionRabbit polyclonal to PECI/ECI2
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Human
- IHC-P: Human liver, kidney and duodenum tissue. WB: RT4 and U-251 MG sp cell lysates. ICC/IF: U-2 OS cells.
This product was previously labelled as PECI
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityImmunogen affinity purified
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Fatty acids
Our Abpromise guarantee covers the use of ab224441 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 44 kDa.|
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100.
FunctionAble to isomerize both 3-cis and 3-trans double bonds into the 2-trans form in a range of enoyl-CoA species. Has a preference for 3-trans substrates.
Tissue specificityAbundant in heart, skeletal muscle and liver. Expressed in CD34(+) T-cells and CD34(+) bone marrow cells.
Sequence similaritiesIn the C-terminal section; belongs to the enoyl-CoA hydratase/isomerase family.
Contains 1 ACB (acyl-CoA-binding) domain.
Cellular localizationMitochondrion and Peroxisome matrix.
- Information by UniProt
- 2-trans-enoyl-CoA isomerase antibody
- ACBD2 antibody
- acyl Coenzyme A binding domain containing 2 antibody
Paraffin-embedded human duodenum tissue stained for PECI/ECI2 using ab224441 at 1/200 dilution in immunohistochemical analysis.
Paraffin-embedded human kidney tissue stained for PECI/ECI2 using ab224441 at 1/200 dilution in immunohistochemical analysis.
Paraffin-embedded human liver tissue stained for PECI/ECI2 using ab224441 at 1/200 dilution in immunohistochemical analysis.
All lanes : Anti-PECI/ECI2 antibody (ab224441) at 1/100 dilution
Lane 1 : RT4 (human urinary bladder cancer cell line) cell lysate
Lane 2 : U-251 MG sp (human brain glioma cell line) cell lysate
Predicted band size: 44 kDa
PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for PECI/ECI2 (green) using ab224441 at 4 µg/ml in ICC/IF.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab224441 has not yet been referenced specifically in any publications.