Anti-PECI/ECI2 antibody (ab229470)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-PECI/ECI2 antibody
See all PECI/ECI2 primary antibodies -
Description
Rabbit polyclonal to PECI/ECI2 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment within Human PECI/ECI2 (internal sequence). The exact sequence is proprietary.
Database link: O75521 -
Positive control
- IHC-P: Human hepatoma tissue. WB: Jurkat, Raji and K562 whole cell lysates.
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General notes
This product was previously labelled as PECI
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab229470 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/100 - 1/1000. | |
| WB | 1/500 - 1/3000. Predicted molecular weight: 44 kDa. |
Target
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Function
Able to isomerize both 3-cis and 3-trans double bonds into the 2-trans form in a range of enoyl-CoA species. Has a preference for 3-trans substrates. -
Tissue specificity
Abundant in heart, skeletal muscle and liver. Expressed in CD34(+) T-cells and CD34(+) bone marrow cells. -
Sequence similarities
In the C-terminal section; belongs to the enoyl-CoA hydratase/isomerase family.
Contains 1 ACB (acyl-CoA-binding) domain. -
Cellular localization
Mitochondrion and Peroxisome matrix. - Information by UniProt
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Database links
- Entrez Gene: 10455 Human
- Omim: 608024 Human
- SwissProt: O75521 Human
- Unigene: 15250 Human
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Alternative names
- 2-trans-enoyl-CoA isomerase antibody
- ACBD2 antibody
- acyl Coenzyme A binding domain containing 2 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PECI/ECI2 antibody (ab229470)Paraffin-embedded human hepatoma tissue stained for PECI/ECI2 using ab229470 at 1/500 dilution in immunohistochemical analysis.
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Western blot - Anti-PECI/ECI2 antibody (ab229470)All lanes : Anti-PECI/ECI2 antibody (ab229470) at 1/1000 dilution
Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 2 : Raji (human Burkitt's lymphoma cell line) whole cell lysate
Lane 3 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 44 kDa10% SDS-PAGE gel.
References
ab229470 has not yet been referenced specifically in any publications.
