Anti-PECI/ECI2 antibody (ab229470)
Key features and details
- Rabbit polyclonal to PECI/ECI2
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PECI/ECI2 antibody
See all PECI/ECI2 primary antibodies -
Description
Rabbit polyclonal to PECI/ECI2 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment within Human PECI/ECI2 (internal sequence). The exact sequence is proprietary.
Database link: O75521 -
Positive control
- IHC-P: Human hepatoma tissue. WB: Jurkat, Raji and K562 whole cell lysates.
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General notes
This product was previously labelled as PECI
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab229470 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/100 - 1/1000. | |
| WB | 1/500 - 1/3000. Predicted molecular weight: 44 kDa. |
Target
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Function
Able to isomerize both 3-cis and 3-trans double bonds into the 2-trans form in a range of enoyl-CoA species. Has a preference for 3-trans substrates. -
Tissue specificity
Abundant in heart, skeletal muscle and liver. Expressed in CD34(+) T-cells and CD34(+) bone marrow cells. -
Sequence similarities
In the C-terminal section; belongs to the enoyl-CoA hydratase/isomerase family.
Contains 1 ACB (acyl-CoA-binding) domain. -
Cellular localization
Mitochondrion and Peroxisome matrix. - Information by UniProt
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Database links
- Entrez Gene: 10455 Human
- Omim: 608024 Human
- SwissProt: O75521 Human
- Unigene: 15250 Human
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Alternative names
- 2-trans-enoyl-CoA isomerase antibody
- ACBD2 antibody
- acyl Coenzyme A binding domain containing 2 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PECI/ECI2 antibody (ab229470)
Paraffin-embedded human hepatoma tissue stained for PECI/ECI2 using ab229470 at 1/500 dilution in immunohistochemical analysis.
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Western blot - Anti-PECI/ECI2 antibody (ab229470)All lanes : Anti-PECI/ECI2 antibody (ab229470) at 1/1000 dilution
Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 2 : Raji (human Burkitt's lymphoma cell line) whole cell lysate
Lane 3 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 44 kDa10% SDS-PAGE gel.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (0)
ab229470 has not yet been referenced specifically in any publications.
