PE/Cy5® Anti-CD11b antibody [M1/70] (ab25533)
![Flow Cytometry - PE/Cy5® Anti-CD11b antibody [M1/70] (ab25533)](https://www.abcam.com/ps/products/25/ab25533/Images/ab25533-276448-anti-cd11b-antibody-m1-70-pe-cy5-flow-cytometry.png "Flow Cytometry - PE/Cy5® Anti-CD11b antibody [M1/70] (ab25533)")
Key features and details
- PE/Cy5® Rat monoclonal [M1/70] to CD11b
- Suitable for: Flow Cyt
- Reacts with: Mouse, Rat, Monkey
- Conjugation: PE/Cy5®. Ex: 496nm, Em: 670nm
- Isotype: IgG2b
Overview
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Product name
PE/Cy5® Anti-CD11b antibody [M1/70]
See all CD11b primary antibodies -
Description
PE/Cy5® Rat monoclonal [M1/70] to CD11b -
Host species
Rat -
Conjugation
PE/Cy5®. Ex: 496nm, Em: 670nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Mouse, Rat, Monkey -
Immunogen
Tissue/ cell preparation (Mouse) - C57BL/10 mouse splenic T cells and concanavalin A activated C57BL/10 splenocytes.
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Positive control
- Mouse spleen cells.
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General notes
This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5,268,486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited.Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituents: 0.1% Gelatin, 0.14% Monobasic dihydrogen sodium phosphate, 0.87% Sodium chloride -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
M1/70 -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
- Alexa Fluor® 488 Anti-CD11b antibody [M1/70] (ab197701)
- Alexa Fluor® 647 Anti-CD11b antibody [M1/70] (ab197702)
- Alexa Fluor® 405 Anti-CD11b antibody [M1/70] (ab197703)
- PerCP/Cy5.5® Anti-CD11b antibody [M1/70] (ab210299)
- PE/Cy7® Anti-CD11b antibody [M1/70] (ab218786)
- PerCP Anti-CD11b antibody [M1/70] (ab237025)
- Anti-CD11b antibody [M1/70] - BSA and Azide free (ab237965)
- violetFluor™ 450 Anti-CD11b antibody [M1/70] (ab242266)
- FITC Anti-CD11b antibody [M1/70] (ab24874)
- PE Anti-CD11b antibody [M1/70] (ab25175)
- redFluor™ 710 Anti-CD11b antibody [M1/70] (ab253091)
- APC Anti-CD11b antibody [M1/70] (ab25482)
- APC/Cy7® Anti-CD11b antibody [M1/70] (ab79096)
- Anti-CD11b antibody [M1/70] (ab8878)
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab25533 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use at an assay dependent concentration. |
Target
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Function
Integrin alpha-M/beta-2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin alpha-M/beta-2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain. -
Tissue specificity
Predominantly expressed in monocytes and granulocytes. -
Involvement in disease
Genetic variations in ITGAM has been associated with susceptibility to systemic lupus erythematosus type 6 (SLEB6) [MIM:609939]. Systemic lupus erythematosus (SLE) is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system. -
Sequence similarities
Belongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats.
Contains 1 VWFA domain. -
Domain
The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 16409 Mouse
- Entrez Gene: 25021 Rat
- SwissProt: P05555 Mouse
- Unigene: 262106 Mouse
- Unigene: 54465 Rat
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Alternative names
- antigen CD11b (p170) antibody
- Antigen CD11b p170 antibody
- CD11 antigen like family member B antibody
see all
Images
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Flow Cytometry - PE/Cy5® Anti-CD11b antibody [M1/70] (ab25533)
Flow Cytometry analysis of C57Bl/6 bone marrow cells labeling CD11b with ab25533 at 0.125 µg (solid line) or Rat IgG2b (PE/Cy5®) isotype control at 0.125 µg (dashed line).
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![Flow Cytometry - PE/Cy5® Anti-CD11b antibody [M1/70] (ab25533)](https://www.abcam.com/ps/products/25/ab25533/Images/ab25533-36039-anti-cd11b-antibody-m1-70-pe-cy5-flow-cytometry.jpg)
Flow Cytometry - PE/Cy5® Anti-CD11b antibody [M1/70] (ab25533)This image is courtesy of an anonymous Abreviewab25533 staining CD11b in Human leukocytes by Flow Cytometry. Cells were prepared for staining after red blood cell lysis and were fixed in paraformaldehyde. The sample was incubated with the primary antibody (1/50 in PBS/BSA 1%) for 30 minutes at 4°C. Gating strategy: Neutrophils. -
![Flow Cytometry - PE/Cy5® Anti-CD11b antibody [M1/70] (ab25533)](https://www.abcam.com/ps/products/25/ab25533/Images/ab25533-36038-anti-cd11b-antibody-m1-70-pe-cy5-flow-cytometry.jpg)
Flow Cytometry - PE/Cy5® Anti-CD11b antibody [M1/70] (ab25533)Scatter plot showing mouse blood leukocyte cells stained with ab25533 (green). The cells were harvested from blood drawn from mice and prepared in PBS/BSA 1%. Cells were fixed in formaldehyde. The cells were then incubated in 1x PBS / 1% BSA to block non-specific protein-protein interactions followed by the antibody (1/100) for 30 min at 4ºC. Isotype control antibody (red) was used under the same conditions. -
![Flow Cytometry - PE/Cy5® Anti-CD11b antibody [M1/70] (ab25533)](https://www.abcam.com/ps/products/25/ab25533/Images/ab25533-36037-anti-cd11b-antibody-m1-70-pe-cy5-flow-cytometry.jpg)
Flow Cytometry - PE/Cy5® Anti-CD11b antibody [M1/70] (ab25533)This image is courtesy of an anonymous Abreviewab25533 staining CD11b in Rat splenocytes by Flow Cytometry. Rat spleen was homogenized and red blood cells were lysed in PBS + 1% BSA and 0.01% sodium azide. The sample was incubated with the primary antibody (1/100 in PBS + 1% BSA and 0.01% sodium azide) for 1 hour at 4°C.
Gating Strategy: Live Macrophages.
Protocols
References (7)
ab25533 has been referenced in 7 publications.
- Wen G et al. Genetic and Pharmacologic Inhibition of the Neutrophil Elastase Inhibits Experimental Atherosclerosis. J Am Heart Assoc 7:N/A (2018). PubMed: 29437605
- Xu J et al. Cobalt (II) ions and nanoparticles induce macrophage retention by ROS-mediated down-regulation of RhoA expression. Acta Biomater 72:434-446 (2018). Flow Cyt . PubMed: 29649639
- Kotla S et al. Heterodimers of the transcriptional factors NFATc3 and FosB mediate tissue factor expression for 15(S)-hydroxyeicosatetraenoic acid-induced monocyte trafficking. J Biol Chem 292:14885-14901 (2017). PubMed: 28724635
- Chen X et al. Retinoic acid facilitates inactivated transmissible gastroenteritis virus induction of CD8(+) T-cell migration to the porcine gut. Sci Rep 6:24152 (2016). Flow Cyt . PubMed: 27080036
- Zeng SL et al. Mesenchymal stem cells abrogate experimental asthma by altering dendritic cell function. Mol Med Rep 12:2511-20 (2015). PubMed: 25936350
- Vrecenak JD et al. Stable long-term mixed chimerism achieved in a canine model of allogeneic in utero hematopoietic cell transplantation. Blood 124:1987-95 (2014). PubMed: 24869940
- Olme CH et al. Live cell detection of chromosome 2 deletion and Sfpi1/PU1 loss in radiation-induced mouse acute myeloid leukaemia. Leuk Res 37:1374-82 (2013). Flow Cyt ; Mouse . PubMed: 23806234
