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  1. Link

    pecy5-cd11b-antibody-m170-ab25533.pdf

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Signal Transduction Cytoskeleton / ECM Cell Adhesion Integrins Alpha
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PE/Cy5® Anti-CD11b antibody [M1/70] (ab25533)

  • Datasheet
  • SDS
Reviews (13)Q&A (1)References (7)

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Flow Cytometry - PE/Cy5® Anti-CD11b antibody [M1/70] (ab25533)
  • Flow Cytometry - PE/Cy5® Anti-CD11b antibody [M1/70] (ab25533)
  • Flow Cytometry - PE/Cy5® Anti-CD11b antibody [M1/70] (ab25533)
  • Flow Cytometry - PE/Cy5® Anti-CD11b antibody [M1/70] (ab25533)

Key features and details

  • PE/Cy5® Rat monoclonal [M1/70] to CD11b
  • Suitable for: Flow Cyt
  • Reacts with: Mouse, Rat, Monkey
  • Conjugation: PE/Cy5®. Ex: 496nm, Em: 670nm
  • Isotype: IgG2b

You may also be interested in

Protein
Recombinant Human CD11b protein (ab126010)

View more associated products

Overview

  • Product name

    PE/Cy5® Anti-CD11b antibody [M1/70]
    See all CD11b primary antibodies
  • Description

    PE/Cy5® Rat monoclonal [M1/70] to CD11b
  • Host species

    Rat
  • Conjugation

    PE/Cy5®. Ex: 496nm, Em: 670nm
  • Tested applications

    Suitable for: Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Monkey
  • Immunogen

    Tissue/ cell preparation (Mouse) - C57BL/10 mouse splenic T cells and concanavalin A activated C57BL/10 splenocytes.

  • Positive control

    • Mouse spleen cells.
  • General notes

    This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5,268,486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.09% Sodium azide
    Constituents: 0.1% Gelatin, 0.14% Monobasic dihydrogen sodium phosphate, 0.87% Sodium chloride
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

    Monoclonal
  • Clone number

    M1/70
  • Isotype

    IgG2b
  • Light chain type

    kappa
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cell Adhesion
    • Integrins
    • Alpha
    • Tags & Cell Markers
    • Cell Type Markers
    • Neuroscience Markers
    • Glial
    • Neuroscience
    • Cell Type Marker
    • Glia marker
    • Microglia marker
    • Stem Cells
    • Mesenchymal Stem Cells
    • Negative Markers
    • Stem Cells
    • Hematopoietic Progenitors
    • Hematopoietic Stem Cells
    • Murine Lineage Negative
    • Stem Cells
    • Hematopoietic Progenitors
    • Lymphoid
    • B Lymphocytic Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Lymphoid
    • T Lymphocytic Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Lymphoid
    • NK Cell Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Myeloid
    • Monocytic Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Myeloid
    • Neutrophil Lineage

Associated products

  • Alternative Versions

    • Alexa Fluor® 488 Anti-CD11b antibody [M1/70] (ab197701)
    • Alexa Fluor® 647 Anti-CD11b antibody [M1/70] (ab197702)
    • Alexa Fluor® 405 Anti-CD11b antibody [M1/70] (ab197703)
    • PerCP/Cy5.5® Anti-CD11b antibody [M1/70] (ab210299)
    • PE/Cy7® Anti-CD11b antibody [M1/70] (ab218786)
    • PerCP Anti-CD11b antibody [M1/70] (ab237025)
    • Anti-CD11b antibody [M1/70] - BSA and Azide free (ab237965)
    • violetFluor™ 450 Anti-CD11b antibody [M1/70] (ab242266)
    • FITC Anti-CD11b antibody [M1/70] (ab24874)
    • PE Anti-CD11b antibody [M1/70] (ab25175)
    • redFluor™ 710 Anti-CD11b antibody [M1/70] (ab253091)
    • APC Anti-CD11b antibody [M1/70] (ab25482)
    • APC/Cy7® Anti-CD11b antibody [M1/70] (ab79096)
    • Anti-CD11b antibody [M1/70] (ab8878)
  • Recombinant Protein

    • Recombinant Human CD11b protein (ab126010)

Applications

Our Abpromise guarantee covers the use of ab25533 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.

Target

  • Function

    Integrin alpha-M/beta-2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin alpha-M/beta-2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain.
  • Tissue specificity

    Predominantly expressed in monocytes and granulocytes.
  • Involvement in disease

    Genetic variations in ITGAM has been associated with susceptibility to systemic lupus erythematosus type 6 (SLEB6) [MIM:609939]. Systemic lupus erythematosus (SLE) is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system.
  • Sequence similarities

    Belongs to the integrin alpha chain family.
    Contains 7 FG-GAP repeats.
    Contains 1 VWFA domain.
  • Domain

    The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage.
  • Cellular localization

    Membrane.
  • Target information above from: UniProt accession P11215 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 16409 Mouse
    • Entrez Gene: 25021 Rat
    • SwissProt: P05555 Mouse
    • Unigene: 262106 Mouse
    • Unigene: 54465 Rat
    • Alternative names

      • antigen CD11b (p170) antibody
      • Antigen CD11b p170 antibody
      • CD11 antigen like family member B antibody
      • CD11 antigen-like family member B antibody
      • CD11b antibody
      • CD11b/CD18 antibody
      • CD49d antibody
      • Cell surface glycoprotein MAC-1 subunit alpha antibody
      • Complement component 3 receptor 3 subunit antibody
      • Complement Component Receptor 3 Alpha antibody
      • Complement receptor type 3 antibody
      • Complement receptor type 3, alpha subunit antibody
      • CR 3 alpha chain (CR3A) antibody
      • CR 3 alpha chain antibody
      • CR-3 alpha chain antibody
      • CR3 antibody
      • CR3A antibody
      • F730045J24Rik antibody
      • Integrin Alpha M antibody
      • Integrin alpha M chain antibody
      • Integrin alpha-M antibody
      • Integrin beta 2 alpha subunit antibody
      • Integrin subunit alpha M antibody
      • integrin, alpha M (complement component 3 receptor 3 subunit) antibody
      • ITAM_HUMAN antibody
      • ITGAM antibody
      • Leukocyte adhesion receptor MO1 antibody
      • Ly-40 antibody
      • MAC 1 antibody
      • Mac-1a antibody
      • MAC1 antibody
      • Mac1, alpha subunit antibody
      • MAC1A antibody
      • Macrophage antigen alpha polypeptide antibody
      • MGC117044 antibody
      • Mo1 antibody
      • Mo1, alpha subunit antibody
      • MO1A antibody
      • Neutrophil adherence receptor alpha M subunit antibody
      • Neutrophil adherence receptor antibody
      • SLEB6 antibody
      see all

    Images

    • Flow Cytometry - PE/Cy5® Anti-CD11b antibody [M1/70] (ab25533)
      Flow Cytometry - PE/Cy5® Anti-CD11b antibody [M1/70] (ab25533)

      Flow Cytometry analysis of C57Bl/6 bone marrow cells labeling CD11b with ab25533 at 0.125 µg (solid line) or Rat IgG2b (PE/Cy5®) isotype control at 0.125 µg (dashed line).

    • Flow Cytometry - PE/Cy5® Anti-CD11b antibody [M1/70] (ab25533)
      Flow Cytometry - PE/Cy5® Anti-CD11b antibody [M1/70] (ab25533)This image is courtesy of an anonymous Abreview
      ab25533 staining CD11b in Human leukocytes by Flow Cytometry. Cells were prepared for staining after red blood cell lysis and were fixed in paraformaldehyde. The sample was incubated with the primary antibody (1/50 in PBS/BSA 1%) for 30 minutes at 4°C. Gating strategy: Neutrophils.

      See Abreview

    • Flow Cytometry - PE/Cy5® Anti-CD11b antibody [M1/70] (ab25533)
      Flow Cytometry - PE/Cy5® Anti-CD11b antibody [M1/70] (ab25533)
      Scatter plot showing mouse blood leukocyte cells stained with ab25533 (green). The cells were harvested from blood drawn from mice and prepared in PBS/BSA 1%. Cells were fixed in formaldehyde. The cells were then incubated in 1x PBS / 1% BSA to block non-specific protein-protein interactions followed by the antibody (1/100) for 30 min at 4ºC. Isotype control antibody (red) was used under the same conditions.
    • Flow Cytometry - PE/Cy5® Anti-CD11b antibody [M1/70] (ab25533)
      Flow Cytometry - PE/Cy5® Anti-CD11b antibody [M1/70] (ab25533)This image is courtesy of an anonymous Abreview
      ab25533 staining CD11b in Rat splenocytes by Flow Cytometry. Rat spleen was homogenized and red blood cells were lysed in PBS + 1% BSA and 0.01% sodium azide. The sample was incubated with the primary antibody (1/100 in PBS + 1% BSA and 0.01% sodium azide) for 1 hour at 4°C.
      Gating Strategy: Live Macrophages.

      See Abreview

    Protocols

    • Flow cytometry protocols
    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (7)

    Publishing research using ab25533? Please let us know so that we can cite the reference in this datasheet.

    ab25533 has been referenced in 7 publications.

    • Wen G  et al. Genetic and Pharmacologic Inhibition of the Neutrophil Elastase Inhibits Experimental Atherosclerosis. J Am Heart Assoc 7:N/A (2018). PubMed: 29437605
    • Xu J  et al. Cobalt (II) ions and nanoparticles induce macrophage retention by ROS-mediated down-regulation of RhoA expression. Acta Biomater 72:434-446 (2018). Flow Cyt . PubMed: 29649639
    • Kotla S  et al. Heterodimers of the transcriptional factors NFATc3 and FosB mediate tissue factor expression for 15(S)-hydroxyeicosatetraenoic acid-induced monocyte trafficking. J Biol Chem 292:14885-14901 (2017). PubMed: 28724635
    • Chen X  et al. Retinoic acid facilitates inactivated transmissible gastroenteritis virus induction of CD8(+) T-cell migration to the porcine gut. Sci Rep 6:24152 (2016). Flow Cyt . PubMed: 27080036
    • Zeng SL  et al. Mesenchymal stem cells abrogate experimental asthma by altering dendritic cell function. Mol Med Rep 12:2511-20 (2015). PubMed: 25936350
    • Vrecenak JD  et al. Stable long-term mixed chimerism achieved in a canine model of allogeneic in utero hematopoietic cell transplantation. Blood 124:1987-95 (2014). PubMed: 24869940
    • Olme CH  et al. Live cell detection of chromosome 2 deletion and Sfpi1/PU1 loss in radiation-induced mouse acute myeloid leukaemia. Leuk Res 37:1374-82 (2013). Flow Cyt ; Mouse . PubMed: 23806234

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-10 of 14 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD11b antibody [M1/70] (PE/Cy5®)

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (bone)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: citrate
    Permeabilization
    Yes - tween 0.1%
    Specification
    bone
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
    Fixative
    Paraformaldehyde
    Read More

    Dr. Szilvia Baron

    Verified customer

    Submitted Jan 21 2019

    Flow Cytometry abreview for Anti-CD11b antibody [M1/70] (PE/Cy5®)

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Fixation
    None
    Permeabilization
    No
    Sample
    Sheep Cell (Peripheral blood)
    Specification
    Peripheral blood
    Gating Strategy
    Monocytes
    Preparation
    Cell harvesting/tissue preparation method: Sodium citrate anticoagulated fresh blood
    Sample buffer: None
    Read More

    Ms. Ina Laura Pieper

    Verified customer

    Submitted Sep 16 2013

    Flow Cytometry abreview for Anti-CD11b antibody [M1/70] (PE/Cy5®)

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Fixation
    None
    Permeabilization
    No
    Sample
    Human Cell (Peripheral blood)
    Specification
    Peripheral blood
    Gating Strategy
    Granulocytes
    Preparation
    Cell harvesting/tissue preparation method: Sodium citrate anticoagulated fresh blood
    Sample buffer: None
    Read More

    Ms. Ina Laura Pieper

    Verified customer

    Submitted Sep 11 2013

    Flow Cytometry abreview for Anti-CD11b antibody [M1/70] (PE/Cy5®)

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Fixation
    None
    Permeabilization
    No
    Sample
    Cow Cell (Peripheral blood)
    Specification
    Peripheral blood
    Gating Strategy
    Monocytes
    Preparation
    Cell harvesting/tissue preparation method: Sodium citrate anticoagulated fresh blood
    Sample buffer: None
    Read More

    Ms. Ina Laura Pieper

    Verified customer

    Submitted Aug 29 2013

    Flow Cytometry abreview for Anti-CD11b antibody [M1/70] (PE/Cy5®)

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Sample
    Human Cell (PBMC)
    Specification
    PBMC
    Preparation
    Cell harvesting/tissue preparation method: Ficoll-hypaque gradrient
    Sample buffer: PBS
    Permeabilization
    No
    Gating Strategy
    lymphocytes
    Read More

    Abcam user community

    Verified customer

    Submitted Feb 19 2013

    Question

    How is product Anti-CD11b antibody [M1/70] (PE/Cy5®) (ab25533) purified?

    Read More

    Abcam community

    Verified customer

    Asked on Jan 17 2013

    Answer

    Product Anti-CD11b antibody [M1/70] (PE/Cy5®) (ab25533) is purified using a Protein G affinity purification column.

    Read More

    Abcam Scientific Support

    Answered on Jan 17 2013

    Flow Cytometry abreview for Anti-CD11b antibody [M1/70] (PE/Cy5®)

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Sample
    Pig Cell (blood leucocytes)
    Specification
    blood leucocytes
    Preparation
    Cell harvesting/tissue preparation method: after red blood cell lysis
    Sample buffer: PBS/BSA 2% sodium azide 0.01%
    Fixation
    Paraformaldehyde
    Permeabilization
    No
    Gating Strategy
    monocytes
    Read More

    Abcam user community

    Verified customer

    Submitted Dec 20 2012

    Flow Cytometry abreview for Anti-CD11b antibody [M1/70] (PE/Cy5®)

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Sample
    Rabbit Cell (blood leucocytes)
    Specification
    blood leucocytes
    Preparation
    Cell harvesting/tissue preparation method: after red blood cell lysis
    Sample buffer: PBS/BSA 2% sodium azide 0.01%
    Fixation
    Paraformaldehyde
    Permeabilization
    No
    Gating Strategy
    monocytes
    Read More

    Abcam user community

    Verified customer

    Submitted Dec 20 2012

    Immunohistochemistry (Frozen sections) abreview for Anti-CD11b antibody [M1/70] (PE/Cy5®)

    Below Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Spleen from immune mouse)
    Specification
    Spleen from immune mouse
    Fixative
    Acetone
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Apr 25 2012

    Flow Cytometry abreview for Anti-CD11b antibody [M1/70] (PE/Cy5®)

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Sample
    Rat Cell (Splenocytes)
    Specification
    Splenocytes
    Preparation
    Cell harvesting/tissue preparation method: spleen was mashed and red blood cells lysed
    Sample buffer: PBS/BSA 1%/Azide 0.01%
    Permeabilization
    No
    Gating Strategy
    alive macrophages
    Read More

    Abcam user community

    Verified customer

    Submitted Apr 13 2012

    1-10 of 14 Abreviews or Q&A

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