PE/Cy5® Anti-CD19 antibody [6D5] (ab25508)
![Flow Cytometry - PE/Cy5® Anti-CD19 antibody [6D5] (ab25508)](https://www.abcam.com/ps/products/25/ab25508/Images/ab25508-272404-anti-cd19-antibody-6d5-pe-cy5-flow-cytometry.jpg "Flow Cytometry - PE/Cy5® Anti-CD19 antibody [6D5] (ab25508)")
Key features and details
- PE/Cy5® Rat monoclonal [6D5] to CD19
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: PE/Cy5®. Ex: 496nm, Em: 670nm
- Isotype: IgG2a
Overview
-
Product name
PE/Cy5® Anti-CD19 antibody [6D5]
See all CD19 primary antibodies -
Description
PE/Cy5® Rat monoclonal [6D5] to CD19 -
Host species
Rat -
Conjugation
PE/Cy5®. Ex: 496nm, Em: 670nm -
Specificity
CD19 B-cell differentiation antigen -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Mouse CD19-expressing K562 human erythroleukemia cells
-
General notes
This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5,268,486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited.Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.1
Preservative: 0.09% Sodium azide
Constituents: PBS, 0.2% Gelatin, 16% Sucrose
Also contains a stabilizing agent. -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
6D5 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Associated products
-
Alternative Versions
Applications
Our Abpromise guarantee covers the use of ab25508 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
IP: Use at an assay dependent dilution.
In vivo and in vitro functional studies:Use at an assay dependent dilution.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
-
Function
Assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation. -
Involvement in disease
Defects in CD19 are the cause of immunodeficiency common variable type 3 (CVID3) [MIM:613493]; also called antibody deficiency due to CD19 defect. CVID3 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B cells is usually in the normal range, but can be low. -
Sequence similarities
Contains 2 Ig-like C2-type (immunoglobulin-like) domains. -
Post-translational
modificationsPhosphorylated on serine and threonine upon DNA damage, probably by ATM or ATR. Phosphorylated on tyrosine following B-cell activation. -
Cellular localization
Membrane. - Information by UniProt
-
Database links
- Entrez Gene: 12478 Mouse
- SwissProt: P25918 Mouse
- Unigene: 4360 Mouse
-
Alternative names
- Antibody deficiency due to defect in CD19 antibody
- Antibody deficiency due to defect in CD19, included antibody
- AW495831 antibody
see all
Images
-
Flow Cytometry - PE/Cy5® Anti-CD19 antibody [6D5] (ab25508)
Flow cytometric analysis of C57BL/6 mouse splenocytes labelling CD19 with ab25508 at 0.01 μg/106 cells and labelling CD4 with a Rat Anti-Mouse CD4-PE antibody (ab25496).
Protocols
Datasheets and documents
References (3)
ab25508 has been referenced in 3 publications.
- Krop I et al. Self-renewal of B-1 lymphocytes is dependent on CD19. Eur J Immunol 26:238-42 (1996). PubMed: 8566073
- Krop I et al. The signaling activity of murine CD19 is regulated during cell development. J Immunol 157:48-56 (1996). PubMed: 8683154
- Fearon DT The CD19-CR2-TAPA-1 complex, CD45 and signaling by the antigen receptor of B lymphocytes. Curr Opin Immunol 5:341-8 (1993). PubMed: 7688513
