PE/Cy5® Anti-CD3 antibody [UCHT1], prediluted (ab157300)
![Flow Cytometry - PE/Cy5® Anti-CD3 antibody [UCHT1], prediluted (ab157300)](https://www.abcam.com/ps/products/157/ab157300/Images/ab157300-279891-anti-cd3-antibody-ucht1-prediluted-pe-cy5-flow-cytometry.jpg "Flow Cytometry - PE/Cy5® Anti-CD3 antibody [UCHT1], prediluted (ab157300)")
Key features and details
- PE/Cy5® Mouse monoclonal [UCHT1] to CD3, prediluted
- Suitable for: Flow Cyt (Intra), Flow Cyt
- Reacts with: Rat, Human
- Conjugation: PE/Cy5®. Ex: 496nm, Em: 670nm
- Isotype: IgG1
Overview
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Product name
PE/Cy5® Anti-CD3 antibody [UCHT1], prediluted
See all CD3 primary antibodies -
Description
PE/Cy5® Mouse monoclonal [UCHT1] to CD3, prediluted -
Host species
Mouse -
Conjugation
PE/Cy5®. Ex: 496nm, Em: 670nm -
Tested applications
Suitable for: Flow Cyt (Intra), Flow Cytmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Tissue, cells or virus corresponding to Human CD3. Human thymocytes followed by Sezary T cells.
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Epitope
ab157300 reacts with the epsilon chain of the CD3 complex. -
Positive control
- Flow Cytometry: human peripheral blood cells. Flow Cyt (Intra): Human blood cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.4
Preservative: 0.1% Sodium azide
Constituents: 99% PBS, 0.2% BSA -
Concentration information loading... -
Purity
Size exclusion -
Purification notes
Purified antibody is conjugated with tandem dye PE/Cy5® under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary. -
Clonality
Monoclonal -
Clone number
UCHT1 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
- PerCP Anti-CD3 antibody [UCHT1], prediluted (ab106215)
- PerCP/Cy5.5® Anti-CD3 antibody [UCHT1], prediluted (ab157317)
- Biotin Anti-CD3 antibody [UCHT1] (ab191112)
- APC Anti-CD3 antibody [UCHT1] (ab239287)
- redFluor™ 710 Anti-CD3 antibody [UCHT1] (ab241935)
- violetFluor™ 450 Anti-CD3 antibody [UCHT1] (ab241941)
- FITC Anti-CD3 antibody [UCHT1] (ab34275)
- PE/Cy7® Anti-CD3 antibody [UCHT1] (ab81992)
- APC/Cy7® Anti-CD3 antibody [UCHT1] (ab82013)
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab157300 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
Use 4µl for 106 cells.
(or 100 µl of whole blood).
ab67435 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
|
| Flow Cyt |
Use 4µl for 106 cells.
(or 100 µl of whole blood).
ab67435 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|
| Notes |
|---|
|
Flow Cyt (Intra)
Use 4µl for 106 cells. (or 100 µl of whole blood).
ab67435 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
|
Flow Cyt
Use 4µl for 106 cells. (or 100 µl of whole blood).
ab67435 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|
Target
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Function
The CD3 complex mediates signal transduction. -
Involvement in disease
Defects in CD3D are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)/B(+)/NK(+) SCID) [MIM:608971]. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development. -
Sequence similarities
Contains 1 ITAM domain. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 915 Human
- Entrez Gene: 916 Human
- Entrez Gene: 917 Human
- Entrez Gene: 919 Human
- Entrez Gene: 25300 Rat
- Entrez Gene: 25710 Rat
- Entrez Gene: 300678 Rat
- Entrez Gene: 315609 Rat
see all -
Alternative names
- 4930549J05Rik antibody
- A430104F18Rik antibody
- AW552088 antibody
see all
Images
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Flow Cytometry - PE/Cy5® Anti-CD3 antibody [UCHT1], prediluted (ab157300)Surface staining of human peripheral blood cells with anti-human CD3 (UCHT1) PE-CyTM5.
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab157300 has been referenced in 5 publications.
- Sui M et al. Establishment of specific cytotoxic T lymphocyte culture system and its inhibitory effect on ovarian cancer. Oncol Lett 12:4087-4093 (2016). Flow Cyt ; Human . PubMed: 27895776
- Siegers GM et al. Different composition of the human and the mouse gammadelta T cell receptor explains different phenotypes of CD3gamma and CD3delta immunodeficiencies. J Exp Med 204:2537-44 (2007). PubMed: 17923503
- Rieux-Laucat F et al. Inherited and somatic CD3zeta mutations in a patient with T-cell deficiency. N Engl J Med 354:1913-21 (2006). PubMed: 16672702
- Arnett KL et al. Crystal structure of a human CD3-epsilon/delta dimer in complex with a UCHT1 single-chain antibody fragment. Proc Natl Acad Sci U S A 101:16268-73 (2004). PubMed: 15534202
- le Gouvello S et al. Serine 16 of stathmin as a cytosolic target for Ca2+/calmodulin-dependent kinase II after CD2 triggering of human T lymphocytes. J Immunol 161:1113-22 (1998). PubMed: 9686569