PE/Cy5® Anti-CD33 antibody [HIM3-4] (ab169823)
![Flow Cytometry - PE/Cy5® Anti-CD33 antibody [HIM3-4] (ab169823)](https://www.abcam.com/ps/products/169/ab169823/Images/ab169823-168508-anti-cd33-antibody-him3-4-pe-cy5-flow-cytometry.jpg "Flow Cytometry - PE/Cy5® Anti-CD33 antibody [HIM3-4] (ab169823)")
Key features and details
- PE/Cy5® Mouse monoclonal [HIM3-4] to CD33
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: PE/Cy5®. Ex: 496nm, Em: 670nm
- Isotype: IgG1
Overview
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Product name
PE/Cy5® Anti-CD33 antibody [HIM3-4]
See all CD33 primary antibodies -
Description
PE/Cy5® Mouse monoclonal [HIM3-4] to CD33 -
Host species
Mouse -
Conjugation
PE/Cy5®. Ex: 496nm, Em: 670nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
The details of the immunogen for this antibody are not available.
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Positive control
- Human normal human peripheral blood cells
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.2
Preservative: 0.09% Sodium azide -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
HIM3-4 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab169823 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
Use 5µl for 106 cells.
ab67435 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
| Notes |
|---|
|
Flow Cyt
Use 5µl for 106 cells. ab67435 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Target
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Function
Putative adhesion molecule of myelomonocytic-derived cells that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. Induces apoptosis in acute myeloid leukemia (in vitro). -
Tissue specificity
Monocytic/myeloid lineage cells. -
Sequence similarities
Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Domain
Contains 2 copies of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases. -
Post-translational
modificationsPhosphorylation of Tyr-340 is involved in binding to PTPN6 and PTPN11. Phosphorylation of Tyr-358 is involved in binding to PTPN6. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 945 Human
- Omim: 159590 Human
- SwissProt: P20138 Human
- Unigene: 83731 Human
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Alternative names
- CD 33 antibody
- CD33 antibody
- CD33 antigen (gp67) antibody
see all
Images
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Flow Cytometry - PE/Cy5® Anti-CD33 antibody [HIM3-4] (ab169823)Flow cytometric analysis of normal human peripheral blood cells labeling CD33 with ab169823 at 5µl/105-106 cells (filled histogram) or PE-Cy5 Mouse IgG1 K Isotype Control (open histogram). Cells in the monocyte gate were used for analysis.
Datasheets and documents
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Datasheet download
References (0)
ab169823 has not yet been referenced specifically in any publications.
