PE/Cy5® Anti-CD40 antibody [1C10] (ab25044)
Key features and details
- PE/Cy5® Rat monoclonal [1C10] to CD40
- Suitable for: IP, Flow Cyt, Functional Studies
- Reacts with: Mouse
- Conjugation: PE/Cy5®. Ex: 496nm, Em: 670nm
- Isotype: IgG2a
Related conjugates and formulations
Overview
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Product name
PE/Cy5® Anti-CD40 antibody [1C10]
See all CD40 primary antibodies -
Description
PE/Cy5® Rat monoclonal [1C10] to CD40 -
Host species
Rat -
Conjugation
PE/Cy5®. Ex: 496nm, Em: 670nm -
Tested applications
Suitable for: IP, Flow Cyt, Functional Studiesmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Recombinant fragment corresponding to CD40. sCD40
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General notes
This antibody closely resembles the gp39/CD40 ligand in its ability to stimulate proliferation of small, resting B lymphocytes in the absence of other cofactors.
This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5, 268, 486 and related patents. Cy® and CyDye® are trademarks of Cytiva.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.1
Preservative: 0.09% Sodium azide
Constituents: PBS, 0.2% Gelatin, 16% Sucrose
Stabilising agent. -
Concentration information loading... -
Purity
Affinity purified -
Primary antibody notes
This antibody closely resembles the gp39/CD40 ligand in its ability to stimulate proliferation of small, resting B lymphocytes in the absence of other cofactors. -
Clonality
Monoclonal -
Clone number
1C10 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab25044 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP |
Use at an assay dependent concentration.
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|
| Flow Cyt |
Use at an assay dependent concentration.
|
|
| Functional Studies |
Use at an assay dependent concentration.
In vitro induction of proliferation of mature B lymphocytes. |
| Notes |
|---|
|
IP
Use at an assay dependent concentration. |
|
Flow Cyt
Use at an assay dependent concentration. |
|
Functional Studies
Use at an assay dependent concentration. In vitro induction of proliferation of mature B lymphocytes. |
Target
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Function
Receptor for TNFSF5/CD40LG. -
Tissue specificity
B-cells and in primary carcinomas. -
Involvement in disease
Defects in CD40 are the cause of hyper-IgM immunodeficiency syndrome type 3 (HIGM3) [MIM:606843]; also known as hyper-IgM syndrome 3. HIGM3 is an autosomal recessive disorder which includes an inability of B cells to undergo isotype switching, one of the final differentiation steps in the humoral immune system, an inability to mount an antibody-specific immune response, and a lack of germinal center formation. -
Sequence similarities
Contains 4 TNFR-Cys repeats. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 21939 Mouse
- SwissProt: P27512 Mouse
- Unigene: 271833 Mouse
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Alternative names
- AI326936 antibody
- B cell associated molecule CD40 antibody
- B cell surface antigen CD40 antibody
see all
Protocols
Datasheets and documents
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Datasheet download
References (1)
ab25044 has been referenced in 1 publication.
- Melo-Baez B et al. MicroRNAs from Extracellular Vesicles Secreted by Bovine Embryos as Early Biomarkers of Developmental Competence. Int J Mol Sci 21:N/A (2020). PubMed: 33255183
