PE/Cy5® Anti-CD45 antibody [HI30], prediluted (ab155385)
![Flow Cytometry - PE/Cy5® Anti-CD45 antibody [HI30], prediluted (ab155385)](https://www.abcam.com/ps/products/155/ab155385/Images/ab155385-162532-anti-cd45-antibody-hi30-prediluted-pe-cy5-flow-cytometry.gif "Flow Cytometry - PE/Cy5® Anti-CD45 antibody [HI30], prediluted (ab155385)")
Key features and details
- PE/Cy5® Mouse monoclonal [HI30] to CD45, prediluted
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: PE/Cy5®. Ex: 496nm, Em: 670nm
- Isotype: IgG1
Overview
-
Product name
PE/Cy5® Anti-CD45 antibody [HI30], prediluted
See all CD45 primary antibodies -
Description
PE/Cy5® Mouse monoclonal [HI30] to CD45, prediluted -
Host species
Mouse -
Conjugation
PE/Cy5®. Ex: 496nm, Em: 670nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
The details of the immunogen for this antibody are not available.
-
Positive control
- Normal Human peripheral blood cells.
-
General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituents: 99% PBS, 0.2% BSA -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
HI30 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
-
Alternative Versions
- Anti-CD45 antibody [HI30] (ab123522)
- PE/Cy7® Anti-CD45 antibody [HI30] (ab155340)
- PE/Cy5.5® Anti-CD45 antibody [HI30] (ab167004)
- APC Anti-CD45 antibody [HI30] (ab210270)
- FITC Anti-CD45 antibody [HI30] (ab210272)
- PE Anti-CD45 antibody [HI30] (ab210273)
- redFluor™ 710 Anti-CD45 antibody [HI30] (ab242257)
- Anti-CD45 antibody [HI30] (ab253085)
-
Isotype control
-
Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab155385 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use 5µl for 106 cells. ab67435 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Target
-
Function
Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. -
Involvement in disease
Defects in PTPRC are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)B(+)NK(+) SCID) [MIM:608971]. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development.
Genetic variations in PTPRC are involved in multiple sclerosis susceptibility (MS) [MIM:126200]. MS is a neurodegenerative disorder characterized by the gradual accumulation of focal plaques of demyelination particularly in the periventricular areas of the brain. Peripheral nerves are not affected. Onset usually in third or fourth decade with intermittent progression over an extended period. The cause is still uncertain. -
Sequence similarities
Belongs to the protein-tyrosine phosphatase family. Receptor class 1/6 subfamily.
Contains 2 fibronectin type-III domains.
Contains 2 tyrosine-protein phosphatase domains. -
Domain
The first PTPase domain interacts with SKAP1. -
Post-translational
modificationsHeavily N- and O-glycosylated. -
Cellular localization
Membrane. Membrane raft. Colocalized with DPP4 in membrane rafts. - Information by UniProt
-
Database links
- Entrez Gene: 5788 Human
- Omim: 151460 Human
- SwissProt: P08575 Human
- Unigene: 654514 Human
-
Alternative names
- B220 antibody
- CD 45 antibody
- CD45 antibody
see all
Images
-
Flow Cytometry - PE/Cy5® Anti-CD45 antibody [HI30], prediluted (ab155385)Flow cytometry analysis of normal Human peripheral blood cells, labeling CD45 with 5µl ab155385 (filled histogram) or Mouse IgG1 Isotype Control (open histogram). Total viable cells were used for analysis.
References (1)
ab155385 has been referenced in 1 publication.
- Wu R et al. Long non-coding RNA HIF1A-AS2 facilitates adipose-derived stem cells (ASCs) osteogenic differentiation through miR-665/IL6 axis via PI3K/Akt signaling pathway. Stem Cell Res Ther 9:348 (2018). PubMed: 30545407
