PE/Cy5® Anti-CD80 antibody [16-10A1] (ab95548)
![Flow Cytometry - PE/Cy5® Anti-CD80 antibody [16-10A1] (ab95548)](https://www.abcam.com/ps/products/95/ab95548/Images/ab95548-109831-anti-cd80-antibody-16-10a1-pe-cy5-flow-cytometry.gif "Flow Cytometry - PE/Cy5® Anti-CD80 antibody [16-10A1] (ab95548)")
Key features and details
- PE/Cy5® Armenian hamster monoclonal [16-10A1] to CD80
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: PE/Cy5®. Ex: 496nm, Em: 670nm
- Isotype: IgG
Related conjugates and formulations
Overview
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Product name
PE/Cy5® Anti-CD80 antibody [16-10A1]
See all CD80 primary antibodies -
Description
PE/Cy5® Armenian hamster monoclonal [16-10A1] to CD80 -
Host species
Armenian hamster -
Conjugation
PE/Cy5®. Ex: 496nm, Em: 670nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Tissue, cells or virus corresponding to Mouse CD80. CHO cell line stably transfected with mouse B7 (CD80) cDNA
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Positive control
- LPS activated C57Bl/6 splenocytes
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituents: 0.87% Sodium chloride, PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
16-10A1 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab95548 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
Use 0.06µg for 105 cells.
Cell number should be determined empirically but can range from 105 to 108 cells/test (based on 100 µl test volume). ab157805 - Armenian Hamster polyclonal IgG, is suitable for use as an isotype control with this antibody. |
| Notes |
|---|
|
Flow Cyt
Use 0.06µg for 105 cells. Cell number should be determined empirically but can range from 105 to 108 cells/test (based on 100 µl test volume). ab157805 - Armenian Hamster polyclonal IgG, is suitable for use as an isotype control with this antibody. |
Target
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Function
Involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28 or CTLA-4 to this receptor. -
Tissue specificity
Expressed on activated B-cells, macrophages and dendritic cells. -
Sequence similarities
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 12519 Mouse
- SwissProt: Q00609 Mouse
- Unigene: 89474 Mouse
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Alternative names
- Activation B7-1 antigen antibody
- B lymphocyte activation antigen B7 antibody
- B7 antibody
see all
Images
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Flow Cytometry - PE/Cy5® Anti-CD80 antibody [16-10A1] (ab95548)Staining of 3 day LPS activated C57Bl/6 splenocytes with 0.03 µg of PE-Cy5 Armenian Hamster IgG Isotype Control (open histogram) or 0.03 µg of ab95548 (filled histogram). Total cells were used for analysis.
Datasheets and documents
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Datasheet download
References (0)
ab95548 has not yet been referenced specifically in any publications.
