PE/Cy5® Anti-CD80 antibody [16-10A1] (ab95548)

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Flow Cytometry - PE/Cy5® Anti-CD80 antibody [16-10A1] (ab95548)

    Key features and details

    • PE/Cy5® Armenian hamster monoclonal [16-10A1] to CD80
    • Suitable for: Flow Cyt
    • Reacts with: Mouse
    • Conjugation: PE/Cy5®. Ex: 496nm, Em: 670nm
    • Isotype: IgG

    Overview

    • Product name

      PE/Cy5® Anti-CD80 antibody [16-10A1]
      See all CD80 primary antibodies

    • Description

      PE/Cy5® Armenian hamster monoclonal [16-10A1] to CD80

    • Host species

      Armenian hamster

    • Conjugation

      PE/Cy5®. Ex: 496nm, Em: 670nm

    • Tested applications

      Suitable for: Flow Cytmore details

    • Species reactivity

      Reacts with: Mouse

    • Immunogen

      CHO cell line stably transfected with mouse B7 (CD80) cDNA

    • Positive control

      • LPS activated C57Bl/6 splenocytes

    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    Applications

    Our Abpromise guarantee covers the use of ab95548 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Flow Cyt Use 0.06µg for 105 cells.

    Cell number should be determined empirically but can range from 105 to 108 cells/test (based on 100 µl test volume).

    ab157805 - Armenian Hamster polyclonal IgG, is suitable for use as an isotype control with this antibody.

    Target

    • Function

      Involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28 or CTLA-4 to this receptor.

    • Tissue specificity

      Expressed on activated B-cells, macrophages and dendritic cells.

    • Sequence similarities

      Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
      Contains 1 Ig-like V-type (immunoglobulin-like) domain.

    • Cellular localization

      Membrane.
    • Information by UniProt

    • Database links

      • Alternative names

        • Activation B7-1 antigen antibody
        • B lymphocyte activation antigen B7 antibody
        • B7 antibody
        • B7-1 antibody
        • B7-1 antigen antibody
        • B7.1 antibody
        • BB1 antibody
        • CD28 antigen ligand 1 antibody
        • CD28LG antibody
        • CD28LG1 antibody
        • CD80 antibody
        • CD80 antigen (CD28 antigen ligand 1, B7-1 antigen) antibody
        • CD80 antigen antibody
        • CD80 molecule antibody
        • CD80_HUMAN antibody
        • Costimulatory factor CD80 antibody
        • costimulatory molecule variant IgV-CD80 antibody
        • CTLA-4 counter-receptor B7.1 antibody
        • LAB7 antibody
        • T-lymphocyte activation antigen CD80 antibody
        see all

      Images

      • Flow Cytometry - PE/Cy5® Anti-CD80 antibody [16-10A1] (ab95548)
        Flow Cytometry - PE/Cy5® Anti-CD80 antibody [16-10A1] (ab95548)
        Staining of 3 day LPS activated C57Bl/6 splenocytes with 0.03 µg of PE-Cy5 Armenian Hamster IgG Isotype Control (open histogram) or 0.03 µg of ab95548 (filled histogram). Total cells were used for analysis.

      References (0)

      Publishing research using ab95548? Please let us know so that we can cite the reference in this datasheet.

      ab95548 has not yet been referenced specifically in any publications.

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