PE/Cy5® Anti-CD80 antibody [2D10.4], prediluted (ab155374)
![Flow Cytometry - PE/Cy5® Anti-CD80 antibody [2D10.4], prediluted (ab155374)](https://www.abcam.com/ps/products/155/ab155374/Images/ab155374-162521-anti-cd80-antibody-2d104-prediluted-pe-cy5-flow-cytometry.jpg "Flow Cytometry - PE/Cy5® Anti-CD80 antibody [2D10.4], prediluted (ab155374)")
Key features and details
- PE/Cy5® Mouse monoclonal [2D10.4] to CD80, prediluted
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: PE/Cy5®. Ex: 496nm, Em: 670nm
- Isotype: IgG1
Overview
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Product name
PE/Cy5® Anti-CD80 antibody [2D10.4], prediluted
See all CD80 primary antibodies -
Description
PE/Cy5® Mouse monoclonal [2D10.4] to CD80, prediluted -
Host species
Mouse -
Conjugation
PE/Cy5®. Ex: 496nm, Em: 670nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
The details of the immunogen for this antibody are not available.
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Positive control
- Normal Human peripheral blood cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituents: 99% PBS, 0.2% BSA -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
2D10.4 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab155374 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
Use 5µl for 106 cells.
ab67435 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
| Notes |
|---|
|
Flow Cyt
Use 5µl for 106 cells. ab67435 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Target
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Function
Involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28 or CTLA-4 to this receptor. -
Tissue specificity
Expressed on activated B-cells, macrophages and dendritic cells. -
Sequence similarities
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 941 Human
- Omim: 112203 Human
- SwissProt: P33681 Human
- Unigene: 838 Human
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Alternative names
- Activation B7-1 antigen antibody
- B lymphocyte activation antigen B7 antibody
- B7 antibody
see all
Images
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Flow Cytometry - PE/Cy5® Anti-CD80 antibody [2D10.4], prediluted (ab155374)Flow Cytometric analysis of normal Human peripheral blood cells labeling CD80 with ab155374 (filled histogram) at 1 µg/test in 100 µl final volume or an equal amount of Mouse IgG1 kappa Isotype Control PE-Cy5 (open histogram). Cells in the lymphocyte gate were used for analysis.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab155374 has been referenced in 1 publication.
- Bie Y et al. Isolation of dendritic cells from umbilical cord blood using magnetic activated cell sorting or adherence. Oncol Lett 10:67-70 (2015). PubMed: 26170978