PE/Cy5® Anti-CD80 antibody [2D10.4], prediluted (ab155374)
![Flow Cytometry - PE/Cy5® Anti-CD80 antibody [2D10.4], prediluted (ab155374)](https://www.abcam.com/ps/products/155/ab155374/Images/ab155374-162521-anti-cd80-antibody-2d104-prediluted-pe-cy5-flow-cytometry.jpg "Flow Cytometry - PE/Cy5® Anti-CD80 antibody [2D10.4], prediluted (ab155374)")
Key features and details
- PE/Cy5® Mouse monoclonal [2D10.4] to CD80, prediluted
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: PE/Cy5®. Ex: 496nm, Em: 670nm
- Isotype: IgG1
Overview
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Product name
PE/Cy5® Anti-CD80 antibody [2D10.4], prediluted
See all CD80 primary antibodies -
Description
PE/Cy5® Mouse monoclonal [2D10.4] to CD80, prediluted -
Host species
Mouse -
Conjugation
PE/Cy5®. Ex: 496nm, Em: 670nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
The details of the immunogen for this antibody are not available.
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Positive control
- Normal Human peripheral blood cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituents: 99% PBS, 0.2% BSA -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
2D10.4 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab155374 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use 5µl for 106 cells. ab67435 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Target
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Function
Involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28 or CTLA-4 to this receptor. -
Tissue specificity
Expressed on activated B-cells, macrophages and dendritic cells. -
Sequence similarities
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 941 Human
- Omim: 112203 Human
- SwissProt: P33681 Human
- Unigene: 838 Human
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Alternative names
- Activation B7-1 antigen antibody
- B lymphocyte activation antigen B7 antibody
- B7 antibody
see all
Images
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Flow Cytometry - PE/Cy5® Anti-CD80 antibody [2D10.4], prediluted (ab155374)Flow Cytometric analysis of normal Human peripheral blood cells labeling CD80 with ab155374 (filled histogram) at 1 µg/test in 100 µl final volume or an equal amount of Mouse IgG1 kappa Isotype Control PE-Cy5 (open histogram). Cells in the lymphocyte gate were used for analysis.
References (1)
ab155374 has been referenced in 1 publication.
- Bie Y et al. Isolation of dendritic cells from umbilical cord blood using magnetic activated cell sorting or adherence. Oncol Lett 10:67-70 (2015). PubMed: 26170978
