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    pecy5-cd90--thy1-antibody-g7-ab25272.pdf

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Immunology Adaptive Immunity T Cells CD
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PE/Cy5® Anti-CD90 / Thy1 antibody [G7] (ab25272)

  • Datasheet
  • SDS
Submit a review Q&A (1)References (7)

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Flow Cytometry - PE/Cy5® Anti-CD90 / Thy1 antibody [G7] (ab25272)

    Key features and details

    • PE/Cy5® Rat monoclonal [G7] to CD90 / Thy1
    • Suitable for: Flow Cyt
    • Reacts with: Mouse
    • Conjugation: PE/Cy5®. Ex: 496nm, Em: 670nm
    • Isotype: IgG2c

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    Overview

    • Product name

      PE/Cy5® Anti-CD90 / Thy1 antibody [G7]
      See all CD90 / Thy1 primary antibodies
    • Description

      PE/Cy5® Rat monoclonal [G7] to CD90 / Thy1
    • Host species

      Rat
    • Conjugation

      PE/Cy5®. Ex: 496nm, Em: 670nm
    • Specificity

      ab25272 is specific for Thy-1.1 and Thy-1.2 alloantigens (Thy-1 epitope region A).
    • Tested applications

      Suitable for: Flow Cytmore details
    • Species reactivity

      Reacts with: Mouse
    • Immunogen

      Tissue, cells or virus corresponding to CD90/ Thy1. Mouse T cell hybridoma C6/G8

    • General notes

      ab25272 stimulates T-cell proliferation and IL-2 secretion, via signalling through the T-cell receptor/CD3 complex. ab25272 has also been reported to promote apoptosis of thymocytes and CTL clones, and to mediate adhesion of thymocytes to thymic stroma.

      This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5, 268, 486 and related patents. Cy® and CyDye® are trademarks of Cytiva.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C.
    • Storage buffer

      pH: 7.1
      Preservative: 0.09% Sodium azide
      Constituents: PBS, 0.2% Gelatin, 16% Sucrose

      Stabilising agent.
    • Concentration information loading...
    • Purity

      Affinity purified
    • Primary antibody notes

      ab25272 stimulates T-cell proliferation and IL-2 secretion, via signalling through the T-cell receptor/CD3 complex. ab25272 has also been reported to promote apoptosis of thymocytes and CTL clones, and to mediate adhesion of thymocytes to thymic stroma.
    • Clonality

      Monoclonal
    • Clone number

      G7
    • Isotype

      IgG2c
    • Light chain type

      kappa
    • Research areas

      • Immunology
      • Adaptive Immunity
      • T Cells
      • CD
      • Neuroscience
      • Cell Type Marker
      • Neuron marker
      • Synapse marker
      • Stem Cells
      • Hematopoietic Progenitors
      • Lymphoid
      • B Lymphocytic Lineage
      • Stem Cells
      • Hematopoietic Progenitors
      • Lymphoid
      • T Lymphocytic Lineage
      • Stem Cells
      • Hematopoietic Progenitors
      • Myeloid
      • Monocytic Lineage

    Associated products

    • Alternative Versions

      • PE Anti-CD90 / Thy1 antibody [G7] (ab24904)
      • Biotin Anti-CD90 / Thy1 antibody [G7] (ab25285)
      • APC Anti-CD90 / Thy1 antibody [G7] (ab25322)
      • FITC Anti-CD90 / Thy1 antibody [G7] (ab25672)
    • Recombinant Protein

      • Recombinant Human CD90 / Thy1 protein (Fc Chimera) (ab157072)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab25272 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Flow Cyt
    Use 0.2µg for 106 cells.
    Notes
    Flow Cyt
    Use 0.2µg for 106 cells.

    Target

    • Function

      May play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain.
    • Sequence similarities

      Contains 1 Ig-like V-type (immunoglobulin-like) domain.
    • Cellular localization

      Cell membrane.
    • Target information above from: UniProt accession P04216 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 21838 Mouse
      • SwissProt: P01831 Mouse
      • Unigene: 3951 Mouse
      • Alternative names

        • CD7 antibody
        • CD90 antibody
        • CD90 antigen antibody
        • CDw90 antibody
        • FLJ33325 antibody
        • MGC128895 antibody
        • T25 antibody
        • Theta antigen antibody
        • Thy 1 antibody
        • Thy 1 cell surface antigen antibody
        • Thy 1 membrane glycoprotein antibody
        • Thy 1 T cell antigen antibody
        • Thy 1.2 antibody
        • Thy-1 antigen antibody
        • Thy-1 membrane glycoprotein antibody
        • Thy1 antibody
        • Thy1 antigen antibody
        • Thy1 T cell antigen antibody
        • Thy1.1 antibody
        • Thy1.2 antibody
        • THY1_HUMAN antibody
        • Thymus cell antigen 1, theta antibody
        see all

      Images

      • Flow Cytometry - PE/Cy5® Anti-CD90 / Thy1 antibody [G7] (ab25272)
        Flow Cytometry - PE/Cy5® Anti-CD90 / Thy1 antibody [G7] (ab25272)

        Flow Cytometry analysis of C57BL/6 mouse splenocytes stained with Anti-CD90 / Thy1 antibody [G7] (PE/Cy5) ab25272 (1 µg/106cells) and Rat Anti-Mouse CD3-epsilon-PE.

      Protocols

      • Flow cytometry protocols
      • Immunohistochemistry protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (7)

      Publishing research using ab25272? Please let us know so that we can cite the reference in this datasheet.

      ab25272 has been referenced in 7 publications.

      • Hu J  et al. Effects of menstrual blood-derived stem cells on endometrial injury repair. Mol Med Rep 19:813-820 (2019). PubMed: 30569163
      • Hueber AO  et al. Thy-1 triggers mouse thymocyte apoptosis through a bcl-2-resistant mechanism. J Exp Med 179:785-96 (1994). PubMed: 7906706
      • Ucker DS  et al. Activation-driven T cell death. II. Quantitative differences alone distinguish stimuli triggering nontransformed T cell proliferation or death. J Immunol 149:1583-92 (1992). PubMed: 1380534
      • He HT  et al. Thy-1 supports adhesion of mouse thymocytes to thymic epithelial cells through a Ca2(+)-independent mechanism. J Exp Med 173:515-8 (1991). PubMed: 1671083
      • Sugiyama E  et al. Selection of T cell receptor expression mutants through the functionally linked Ly-6A. Cell Immunol 130:271-80 (1990). PubMed: 1976441
      • Tentori L  et al. Proliferation and production of IL-2 and B cell stimulatory factor 1/IL-4 in early fetal thymocytes by activation through Thy-1 and CD3. J Immunol 140:1089-94 (1988). PubMed: 2893823
      • Gunter KC  et al. T cell-activating properties of an anti-Thy-1 monoclonal antibody. Possible analogy to OKT3/Leu-4. J Exp Med 159:716-30 (1984). PubMed: 6142077

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      Question

      likely reactivity given sequence similarity of immunogen sequence with the human homolog

      Read More

      Abcam community

      Verified customer

      Asked on Jun 29 2006

      Answer

      Thank you for your enquiry. I have been in correspondence with the source of this antibody and unfortunately they in turn source this antibody. They have informed me that they have not been provided with details of the immunogen from their originator and have not mapped the epitope themselves. Therefore unfortunately I cannot provide you with a prediction of the sequence similarity and infer the likely reactivity with human. I am sorry I can not be of more assistance but at present all I can determine is that this antibody has been tested against mouse.

      Read More

      Abcam Scientific Support

      Answered on Jun 30 2006

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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