PE/Cy5® Anti-CD90 / Thy1 antibody [G7] (ab25272)
![Flow Cytometry - PE/Cy5® Anti-CD90 / Thy1 antibody [G7] (ab25272)](https://www.abcam.com/ps/products/25/ab25272/Images/ab25272-272339-anti-cd90-thy1-antibody-g7-pe-cy5-flow-cytometry.jpg "Flow Cytometry - PE/Cy5® Anti-CD90 / Thy1 antibody [G7] (ab25272)")
Key features and details
- PE/Cy5® Rat monoclonal [G7] to CD90 / Thy1
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: PE/Cy5®. Ex: 496nm, Em: 670nm
- Isotype: IgG2c
Overview
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Product name
PE/Cy5® Anti-CD90 / Thy1 antibody [G7]
See all CD90 / Thy1 primary antibodies -
Description
PE/Cy5® Rat monoclonal [G7] to CD90 / Thy1 -
Host species
Rat -
Conjugation
PE/Cy5®. Ex: 496nm, Em: 670nm -
Specificity
ab25272 is specific for Thy-1.1 and Thy-1.2 alloantigens (Thy-1 epitope region A). -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
The details of the immunogen for this antibody are not available.
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General notes
This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5,268,486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited.ab25272 stimulates T-cell proliferation and IL-2 secretion, via signalling through the T-cell receptor/CD3 complex. ab25272 has also been reported to promote apoptosis of thymocytes and CTL clones, and to mediate adhesion of thymocytes to thymic stroma.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.1
Preservative: 0.09% Sodium azide
Constituents: PBS, 0.2% Gelatin, 16% Sucrose
Stabilising agent. -
Concentration information loading... -
Purity
Affinity purified -
Primary antibody notes
ab25272 stimulates T-cell proliferation and IL-2 secretion, via signalling through the T-cell receptor/CD3 complex. ab25272 has also been reported to promote apoptosis of thymocytes and CTL clones, and to mediate adhesion of thymocytes to thymic stroma. -
Clonality
Monoclonal -
Clone number
G7 -
Isotype
IgG2c -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab25272 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use 0.2µg for 106 cells. |
Target
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Function
May play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain. -
Sequence similarities
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 21838 Mouse
- SwissProt: P01831 Mouse
- Unigene: 3951 Mouse
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Alternative names
- CD7 antibody
- CD90 antibody
- CD90 antigen antibody
see all
Images
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Flow Cytometry - PE/Cy5® Anti-CD90 / Thy1 antibody [G7] (ab25272)
Flow Cytometry analysis of C57BL/6 mouse splenocytes stained with Anti-CD90 / Thy1 antibody [G7] (PE/Cy5) ab25272 (1 µg/106cells) and Rat Anti-Mouse CD3-epsilon-PE.
Protocols
References (7)
ab25272 has been referenced in 7 publications.
- Hu J et al. Effects of menstrual blood-derived stem cells on endometrial injury repair. Mol Med Rep 19:813-820 (2019). PubMed: 30569163
- Hueber AO et al. Thy-1 triggers mouse thymocyte apoptosis through a bcl-2-resistant mechanism. J Exp Med 179:785-96 (1994). PubMed: 7906706
- Ucker DS et al. Activation-driven T cell death. II. Quantitative differences alone distinguish stimuli triggering nontransformed T cell proliferation or death. J Immunol 149:1583-92 (1992). PubMed: 1380534
- He HT et al. Thy-1 supports adhesion of mouse thymocytes to thymic epithelial cells through a Ca2(+)-independent mechanism. J Exp Med 173:515-8 (1991). PubMed: 1671083
- Sugiyama E et al. Selection of T cell receptor expression mutants through the functionally linked Ly-6A. Cell Immunol 130:271-80 (1990). PubMed: 1976441
- Tentori L et al. Proliferation and production of IL-2 and B cell stimulatory factor 1/IL-4 in early fetal thymocytes by activation through Thy-1 and CD3. J Immunol 140:1089-94 (1988). PubMed: 2893823
- Gunter KC et al. T cell-activating properties of an anti-Thy-1 monoclonal antibody. Possible analogy to OKT3/Leu-4. J Exp Med 159:716-30 (1984). PubMed: 6142077
