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Cardiovascular Blood Blood Cell Antigens RBC Antigens
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PE/Cy5® Anti-Glycophorin A antibody [JC159] (ab201309)

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Key features and details

  • PE/Cy5® Mouse monoclonal [JC159] to Glycophorin A
  • Reacts with: Rat, Human
  • Conjugation: PE/Cy5®. Ex: 496nm, Em: 670nm
  • Isotype: IgG1

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Protein
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Recombinant Human Glycophorin A protein (ab114330)
Primary
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Anti-Glycophorin A antibody [EPR8200] - BSA and Azide free (ab218372)

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Overview

  • Product name

    PE/Cy5® Anti-Glycophorin A antibody [JC159]
    See all Glycophorin A primary antibodies
  • Description

    PE/Cy5® Mouse monoclonal [JC159] to Glycophorin A
  • Host species

    Mouse
  • Conjugation

    PE/Cy5®. Ex: 496nm, Em: 670nm
  • Specificity

    ab201309 does not react with Glycophorin B.
  • Species reactivity

    Reacts with: Rat, Human
  • Immunogen

    Tissue, cells or virus corresponding to Human Glycophorin A. (Membrane preparation from splenic hairy cell leukemia.)
    Database link: P02724

  • Epitope

    ab201309 recognizes an epitope between amino acids 27 and 40 of the extracellular portion of Glycophorin A (MHTSTSSSVTKSYI).
  • Positive control

    • Human blood cells

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at +4°C. Do Not Freeze. Store In the Dark.
  • Storage buffer

    pH: 7.4
    Preservative: 0.0975% Sodium azide
    Constituents: 99% PBS, 0.2% BSA
  • Concentration information loading...
  • Purity

    Size exclusion
  • Clonality

    Monoclonal
  • Clone number

    JC159
  • Isotype

    IgG1
  • Research areas

    • Cardiovascular
    • Blood
    • Blood Cell Antigens
    • RBC Antigens
    • Cardiovascular
    • Blood
    • Blood Cell Antigens
    • RBC Antigens
    • Blood Group Antigens

Associated products

  • Alternative Versions

    • PE Anti-Glycophorin A antibody [JC159], prediluted (ab197142)
    • APC/Cy7® Anti-Glycophorin A antibody [JC159] (ab233456)
    • FITC Anti-Glycophorin A antibody [JC159] (ab233580)
    • Anti-Glycophorin A antibody [JC159], prediluted (ab7503)
  • Isotype control

    • PE/Cy5® Mouse IgG1, kappa monoclonal [15H6] - Isotype control (ab67435)
  • Recombinant Protein

    • Recombinant Human Glycophorin A protein (ab114330)
  • Related Products

    • Recombinant Human Glycophorin A protein (ab114330)

Target

  • Function

    Glycophorin A is the major intrinsic membrane protein of the erythrocyte. The N-terminal glycosylated segment, which lies outside the erythrocyte membrane, has MN blood group receptors. Appears to be important for the function of SLC4A1 and is required for high activity of SLC4A1. May be involved in translocation of SLC4A1 to the plasma membrane. Is a receptor for influenza virus. Is a receptor for Plasmodium falciparum erythrocyte-binding antigen 175 (EBA-175); binding of EBA-175 is dependent on sialic acid residues of the O-linked glycans. Appears to be a receptor for Hepatitis A virus (HAV).
  • Sequence similarities

    Belongs to the glycophorin A family.
  • Post-translational
    modifications

    The major O-linked glycan are NeuAc-alpha-(2-3)-Gal-beta-(1-3)-[NeuAc-alpha-(2-6)]-GalNacOH (about 78 %) and NeuAc-alpha-(2-3)-Gal-beta-(1-3)-GalNAcOH (17 %). Minor O-glycans (5 %) include NeuAc-alpha-(2-3)-Gal-beta-(1-3)-[NeuAc-alpha-(2-6)]-GalNAcOH NeuAc-alpha-(2-8)-NeuAc-alpha-(2-3)-Gal-beta-(1-3)-GalNAcOH. About 1% of all O-linked glycans carry blood group A, B and H determinants. They derive from a type-2 precursor core structure, Gal-beta-(1,3)-GlcNAc-beta-1-R, and the antigens are synthesized by addition of fucose (H antigen-specific) and then N-acetylgalactosamine (A antigen-specific) or galactose (B antigen-specific). Specifically O-linked-glycans are NeuAc-alpha-(2-3)-Gal-beta-(1-3)-GalNAcOH-(6-1)-GlcNAc-beta-(4-1)-[Fuc-alpha-(1-2)]-Gal-beta-(3-1)-GalNAc-alpha (about 1%, B antigen-specific) and NeuAc-alpha-(2-3)-Gal-beta-(1-3)-GalNAcOH-(6-1)-GlcNAc-beta-(4-1)-[Fuc-alpha-(1-2)]-Gal-beta (1 %, O antigen-, A antigen- and B antigen-specific).
  • Cellular localization

    Cell membrane. Appears to be colocalized with SLC4A1.
  • Target information above from: UniProt accession P02724 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 2993 Human
    • Entrez Gene: 688972 Rat
    • Omim: 111300 Human
    • SwissProt: P02724 Human
    • Unigene: 434973 Human
    • Alternative names

      • AI853584 antibody
      • Blood group--MN locus antibody
      • CD_antigen=CD235a antibody
      • CD235a antibody
      • GLPA_HUMAN antibody
      • Glycophorin A (MNS blood group) antibody
      • Glycophorin A antibody
      • Glycophorin A, included antibody
      • Glycophorin-A antibody
      • GlycophorinA antibody
      • GPA antibody
      • GPErik antibody
      • GpMiIII antibody
      • GPSAT antibody
      • GYPA antibody
      • GYPA, included antibody
      • HGpMiIII antibody
      • HGpMiV antibody
      • HGpMiX antibody
      • HGpMiXI antibody
      • HGpSta(C) antibody
      • MN antibody
      • MN sialoglycoprotein antibody
      • MNS antibody
      • PAS-2 antibody
      • PAS2 antibody
      • Sialoglycoprotein alpha antibody
      see all

    Protocols

    • Flow cytometry protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

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    References (0)

    Publishing research using ab201309? Please let us know so that we can cite the reference in this datasheet.

    ab201309 has not yet been referenced specifically in any publications.

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