PE/Cy5® Anti-Glycophorin A antibody [JC159] (ab201309)
Key features and details
- PE/Cy5® Mouse monoclonal [JC159] to Glycophorin A
- Reacts with: Rat, Human
- Conjugation: PE/Cy5®. Ex: 496nm, Em: 670nm
- Isotype: IgG1
Overview
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Product name
PE/Cy5® Anti-Glycophorin A antibody [JC159]
See all Glycophorin A primary antibodies -
Description
PE/Cy5® Mouse monoclonal [JC159] to Glycophorin A -
Host species
Mouse -
Conjugation
PE/Cy5®. Ex: 496nm, Em: 670nm -
Specificity
ab201309 does not react with Glycophorin B. -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Tissue, cells or virus corresponding to Human Glycophorin A. (Membrane preparation from splenic hairy cell leukemia.)
Database link: P02724 -
Epitope
ab201309 recognizes an epitope between amino acids 27 and 40 of the extracellular portion of Glycophorin A (MHTSTSSSVTKSYI). -
Positive control
- Human blood cells
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at +4°C. Do Not Freeze. Store In the Dark. -
Storage buffer
pH: 7.4
Preservative: 0.0975% Sodium azide
Constituents: 99% PBS, 0.2% BSA -
Concentration information loading... -
Purity
Size exclusion -
Clonality
Monoclonal -
Clone number
JC159 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
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Related Products
Target
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Function
Glycophorin A is the major intrinsic membrane protein of the erythrocyte. The N-terminal glycosylated segment, which lies outside the erythrocyte membrane, has MN blood group receptors. Appears to be important for the function of SLC4A1 and is required for high activity of SLC4A1. May be involved in translocation of SLC4A1 to the plasma membrane. Is a receptor for influenza virus. Is a receptor for Plasmodium falciparum erythrocyte-binding antigen 175 (EBA-175); binding of EBA-175 is dependent on sialic acid residues of the O-linked glycans. Appears to be a receptor for Hepatitis A virus (HAV). -
Sequence similarities
Belongs to the glycophorin A family. -
Post-translational
modificationsThe major O-linked glycan are NeuAc-alpha-(2-3)-Gal-beta-(1-3)-[NeuAc-alpha-(2-6)]-GalNacOH (about 78 %) and NeuAc-alpha-(2-3)-Gal-beta-(1-3)-GalNAcOH (17 %). Minor O-glycans (5 %) include NeuAc-alpha-(2-3)-Gal-beta-(1-3)-[NeuAc-alpha-(2-6)]-GalNAcOH NeuAc-alpha-(2-8)-NeuAc-alpha-(2-3)-Gal-beta-(1-3)-GalNAcOH. About 1% of all O-linked glycans carry blood group A, B and H determinants. They derive from a type-2 precursor core structure, Gal-beta-(1,3)-GlcNAc-beta-1-R, and the antigens are synthesized by addition of fucose (H antigen-specific) and then N-acetylgalactosamine (A antigen-specific) or galactose (B antigen-specific). Specifically O-linked-glycans are NeuAc-alpha-(2-3)-Gal-beta-(1-3)-GalNAcOH-(6-1)-GlcNAc-beta-(4-1)-[Fuc-alpha-(1-2)]-Gal-beta-(3-1)-GalNAc-alpha (about 1%, B antigen-specific) and NeuAc-alpha-(2-3)-Gal-beta-(1-3)-GalNAcOH-(6-1)-GlcNAc-beta-(4-1)-[Fuc-alpha-(1-2)]-Gal-beta (1 %, O antigen-, A antigen- and B antigen-specific). -
Cellular localization
Cell membrane. Appears to be colocalized with SLC4A1. - Information by UniProt
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Database links
- Entrez Gene: 2993 Human
- Entrez Gene: 688972 Rat
- Omim: 111300 Human
- SwissProt: P02724 Human
- Unigene: 434973 Human
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Alternative names
- AI853584 antibody
- Blood group--MN locus antibody
- CD_antigen=CD235a antibody
see all
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab201309 has not yet been referenced specifically in any publications.