PE/Cy5® Anti-IgE antibody [BE5] (ab233570)

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Flow Cytometry - PE/Cy5® Anti-IgE antibody [BE5] (ab233570)

    Key features and details

    • PE/Cy5® Mouse monoclonal [BE5] to IgE
    • Suitable for: Flow Cyt
    • Reacts with: Human
    • Conjugation: PE/Cy5®. Ex: 496nm, Em: 670nm
    • Isotype: IgG1

    Overview

    • Product name

      PE/Cy5® Anti-IgE antibody [BE5]
      See all IgE primary antibodies

    • Description

      PE/Cy5® Mouse monoclonal [BE5] to IgE

    • Host species

      Mouse

    • Conjugation

      PE/Cy5®. Ex: 496nm, Em: 670nm

    • Tested applications

      Suitable for: Flow Cytmore details

    • Species reactivity

      Reacts with: Human

    • Immunogen

      Full length native protein (purified) corresponding to Human IgE. Purified.

    • Positive control

      • Flow Cytometry: Human peripheral blood cells.

    • General notes

      This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5, 268, 486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited.

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid

    • Storage instructions

      Shipped at 4°C. Store at +4°C. Store In the Dark.

    • Storage buffer

      pH: 7.4
      Preservative: 0.0975% Sodium azide
      Constituent: PBS
    • Concentration information loading...

    • Purity

      Size exclusion

    • Clonality

      Monoclonal

    • Clone number

      BE5

    • Isotype

      IgG1

    • Research areas

    Applications

    Our Abpromise guarantee covers the use of ab233570 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Flow Cyt Use a concentration of 5 µg/ml.

    Target

    • Relevance

      IgE is the class of antibodies produced in the lungs, skin, and mucous membranes. It may protect against parasite invasion, but it is a major factor in allergic reactions. The antigen-specific IgE interacts with mast cells and eosinophils, triggers the release of histamine, leukotrienes and other substances that lead to the itching, sneezing and congestion of allergies - and the life threatening respiratory distress of asthma and anaphylactic shock.

    • Cellular localization

      Cell Membrane

    • Database links

      • Alternative names

        • Igh2 antibody
        • IGHE antibody
        • IGHEP1 antibody
        • Immunoglobulin heavy constant epsilon antibody

      Images

      • Flow Cytometry - PE/Cy5® Anti-IgE antibody [BE5] (ab233570)
        Flow Cytometry - PE/Cy5® Anti-IgE antibody [BE5] (ab233570)

        Surface staining of IgE in human peripheral blood cells with ab233570 (3 μg/ml). Gated on leukocytes.

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      References (0)

      Publishing research using ab233570? Please let us know so that we can cite the reference in this datasheet.

      ab233570 has not yet been referenced specifically in any publications.

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