PE/Cy5® Anti-IgG antibody [4A11] (ab239260)

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Flow Cytometry - PE/Cy5® Anti-IgG antibody [4A11] (ab239260)

    Key features and details

    • PE/Cy5® Mouse monoclonal [4A11] to IgG
    • Suitable for: Flow Cyt
    • Reacts with: Human
    • Conjugation: PE/Cy5®. Ex: 496nm, Em: 670nm
    • Isotype: IgG2b

    Overview

    • Product name

      PE/Cy5® Anti-IgG antibody [4A11]
      See all IgG primary antibodies

    • Description

      PE/Cy5® Mouse monoclonal [4A11] to IgG

    • Host species

      Mouse

    • Conjugation

      PE/Cy5®. Ex: 496nm, Em: 670nm

    • Specificity

      ab239260 reacts with Fab fragment of human IgG.

    • Tested applications

      Suitable for: Flow Cytmore details

    • Species reactivity

      Reacts with: Human

    • Immunogen

      Full length protein corresponding to Human IgG. (Purified).

    • Positive control

      • Flow Cyt: Human peripheral blood cells.

    • General notes

      This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5, 268, 486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited.

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid

    • Storage instructions

      Shipped at 4°C. Store at +4°C. Store In the Dark.

    • Storage buffer

      pH: 7.4
      Preservative: 0.0975% Sodium azide
      Constituent: PBS
    • Concentration information loading...

    • Purity

      Size exclusion

    • Purification notes

      Purified antibody is conjugated with tandem dye PE/Cy5® under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.

    • Clonality

      Monoclonal

    • Clone number

      4A11

    • Isotype

      IgG2b

    • Research areas

    Applications

    Our Abpromise guarantee covers the use of ab239260 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Flow Cyt Use 4µl for 106 cells.

    (or 100 μl of whole blood).

    Target

    Images

    • Flow Cytometry - PE/Cy5® Anti-IgG antibody [4A11] (ab239260)
      Flow Cytometry - PE/Cy5® Anti-IgG antibody [4A11] (ab239260)

      Flow cytometric analysis of human peripheral blood cells labeling IgG with ab239260. Surface staining. Gated on leukocytes.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab239260? Please let us know so that we can cite the reference in this datasheet.

    ab239260 has not yet been referenced specifically in any publications.

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