Question (60878) | PE/Cy5® Conjugation Kit (ab102893)

Go to datasheet (ab102893)

Question

Are any of the reagents in the EasyLink conjugation kit toxic to cells?

Answer

One more thing I learned from the lab, if you are still concerned about the effect of the EasyLink reagents on live cells:

"We commonly perform overnight conjugations here without adding the Quencher in the morning. The reactive chemicals have a short half-life, and should already be inactivated after an overnight conjugation. Unfortunately, we do not have any data on the effect of the kit components on live cells."

As I mentioned in my earlier e-mail, many customers follow the shorter incubation protocol on the datasheet and stain live cells successfully.

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