Question (75367) | PE/Cy5® Conjugation Kit (ab102893)

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Question

Inquiry: I would like to ask you about the sample preparation. I bought ab102893 PE/Cy5 Conjugation kit 100 ug, Lot: GR151075-3. The protocol recommend using 100ul of antibody with 100ug kit formats. And the recommended antibody concentration is 1-4 mg/ml. But the concentration of antibody that I can use is 0.3 mg/ml. Can I process as the conjugation protocol? Or, do you have any suggestion?

Answer

We wouldn’t recommend using the kits with an antibody at such a low concentration as the conjugation reaction will result in an over labelled antibody. We offer antibody concentration and purification kits: https://www.abcam.com/antibody-purification-kit-protein-a-ab102784.html or

https://www.abcam.com/antibody-purification-kit-protein-g-ab128747.html

Please select the kit based on the species of the secondary antibodies. Please see the table at the bottom of this webpage https://www.abcam.com/index.html?pageconfig=resource&rid=11385

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