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Inquiry: I would like to ask you about the sample preparation. I bought ab102893 PE/Cy5 Conjugation kit 100 ug, Lot: GR151075-3. The protocol recommend using 100ul of antibody with 100ug kit formats. And the recommended antibody concentration is 1-4 mg/ml. But the concentration of antibody that I can use is 0.3 mg/ml. Can I process as the conjugation protocol? Or, do you have any suggestion?
Asked on Jan 17 2014
We wouldn’t recommend using the kits with an antibody at such a low concentration as the conjugation reaction will result in an over labelled antibody. We offer antibody concentration and purification kits: https://www.abcam.com/antibody-purification-kit-protein-a-ab102784.html or
Please select the kit based on the species of the secondary antibodies. Please see the table at the bottom of this webpage https://www.abcam.com/index.html?pageconfig=resource&rid=11385
Padamjeet SinghAbcam Scientific Support
Answered on Jan 17 2014