PE/Cy7® Anti-c-Kit antibody [104D2] (ab233584)

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Flow Cytometry - PE/Cy7® Anti-c-Kit antibody [104D2] (ab233584)

    Key features and details

    • PE/Cy7® Mouse monoclonal [104D2] to c-Kit
    • Suitable for: Flow Cyt
    • Reacts with: Cow, Human, Non human primates
    • Conjugation: PE/Cy7®. Ex: 496nm, Em: 774nm
    • Isotype: IgG1

    Overview

    • Product name

      PE/Cy7® Anti-c-Kit antibody [104D2]
      See all c-Kit primary antibodies

    • Description

      PE/Cy7® Mouse monoclonal [104D2] to c-Kit

    • Host species

      Mouse

    • Conjugation

      PE/Cy7®. Ex: 496nm, Em: 774nm

    • Tested applications

      Suitable for: Flow Cytmore details

    • Species reactivity

      Reacts with: Cow, Human, Non human primates

    • Immunogen

      Tissue, cells or virus corresponding to c-Kit. MOLM-1 megakaryocytic cells.

    • Positive control

      • Flow Cytometry: Human peripheral blood cells.

    • General notes

      This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5, 268, 486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited.

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    Applications

    Our Abpromise guarantee covers the use of ab233584 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Flow Cyt Use 4µl for 106 cells.

    (or 100 uL whole blood)

    Target

    • Function

      Tyrosine-protein kinase that acts as cell-surface receptor for the cytokine KITLG/SCF and plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. In response to KITLG/SCF binding, KIT can activate several signaling pathways. Phosphorylates PIK3R1, PLCG1, SH2B2/APS and CBL. Activates the AKT1 signaling pathway by phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase. Activated KIT also transmits signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. Promotes activation of STAT family members STAT1, STAT3, STAT5A and STAT5B. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KIT signaling is modulated by protein phosphatases, and by rapid internalization and degradation of the receptor. Activated KIT promotes phosphorylation of the protein phosphatases PTPN6/SHP-1 and PTPRU, and of the transcription factors STAT1, STAT3, STAT5A and STAT5B. Promotes phosphorylation of PIK3R1, CBL, CRK (isoform Crk-II), LYN, MAPK1/ERK2 and/or MAPK3/ERK1, PLCG1, SRC and SHC1.

    • Tissue specificity

      Isoform 1 and isoform 2 are detected in spermatogonia and Leydig cells. Isoform 3 is detected in round spermatids, elongating spermatids and spermatozoa (at protein level). Widely expressed. Detected in the hematopoietic system, the gastrointestinal system, in melanocytes and in germ cells.

    • Involvement in disease

      Piebald trait
      Gastrointestinal stromal tumor
      Testicular germ cell tumor
      Leukemia, acute myelogenous

    • Sequence similarities

      Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
      Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
      Contains 1 protein kinase domain.

    • Post-translational
      modifications

      Ubiquitinated by SOCS6. KIT is rapidly ubiquitinated after autophosphorylation induced by KITLG/SCF binding, leading to internalization and degradation.
      Autophosphorylated on tyrosine residues. KITLG/SCF binding enhances autophosphorylation. Isoform 1 shows low levels of tyrosine phosphorylation in the absence of added KITLG/SCF (in vitro). Kinase activity is down-regulated by phosphorylation on serine residues by protein kinase C family members. Phosphorylation at Tyr-568 is required for interaction with PTPN11/SHP-2, CRK (isoform Crk-II) and members of the SRC tyrosine-protein kinase family. Phosphorylation at Tyr-570 is required for interaction with PTPN6/SHP-1. Phosphorylation at Tyr-703, Tyr-823 and Tyr-936 is important for interaction with GRB2. Phosphorylation at Tyr-721 is important for interaction with PIK3R1. Phosphorylation at Tyr-823 and Tyr-936 is important for interaction with GRB7.

    • Cellular localization

      Cell membrane and Cytoplasm. Detected in the cytoplasm of spermatozoa, especially in the equatorial and subacrosomal region of the sperm head.
    • Information by UniProt

    • Database links

      • Alternative names

        • C Kit antibody
        • c-Kit antibody
        • c-Kit Ligand antibody
        • CD117 antibody
        • Kit antibody
        • Kit Ligand antibody
        • KIT oncogene antibody
        • KIT proto oncogene receptor tyrosine kinase antibody
        • KIT_HUMAN antibody
        • Mast cell growth factor receptor antibody
        • Mast/stem cell growth factor receptor Kit antibody
        • MGF antibody
        • p145 c-kit antibody
        • PBT antibody
        • Piebald trait protein antibody
        • Proto oncogene c Kit antibody
        • Proto oncogene tyrosine protein kinase Kit antibody
        • Proto-oncogene c-Kit antibody
        • SCF Receptor antibody
        • SCFR antibody
        • soluble KIT variant 1 antibody
        • Steel Factor Receptor antibody
        • Stem cell factor receptor antibody
        • tyrosine protein kinase Kit antibody
        • Tyrosine-protein kinase Kit antibody
        • v kit Hardy Zuckerman 4 feline sarcoma viral oncogene homolog antibody
        • v kit Hardy Zuckerman 4 feline sarcoma viral oncogene like protein antibody
        • v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog antibody
        see all

      Images

      • Flow Cytometry - PE/Cy7® Anti-c-Kit antibody [104D2] (ab233584)
        Flow Cytometry - PE/Cy7® Anti-c-Kit antibody [104D2] (ab233584)

        Flow cytometric surface staining of human peripheral blood cells, labeling c-Kit (CD117) with ab233584. Leukocyte gate.

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      References (0)

      Publishing research using ab233584? Please let us know so that we can cite the reference in this datasheet.

      ab233584 has not yet been referenced specifically in any publications.

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