PE/Cy7® Anti-CD14 antibody [61D3], prediluted (ab155360)

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Flow Cytometry - PE/Cy7® Anti-CD14 antibody [61D3], prediluted (ab155360)

    Key features and details

    • PE/Cy7® Mouse monoclonal [61D3] to CD14, prediluted
    • Suitable for: Flow Cyt
    • Reacts with: Human
    • Conjugation: PE/Cy7®. Ex: 496nm, Em: 774nm
    • Isotype: IgG1

    Overview

    • Product name

      PE/Cy7® Anti-CD14 antibody [61D3], prediluted
      See all CD14 primary antibodies

    • Description

      PE/Cy7® Mouse monoclonal [61D3] to CD14, prediluted

    • Host species

      Mouse

    • Conjugation

      PE/Cy7®. Ex: 496nm, Em: 774nm

    • Tested applications

      Suitable for: Flow Cytmore details

    • Species reactivity

      Reacts with: Human

    • Immunogen

      The details of the immunogen for this antibody are not available.

    • Positive control

      • Normal Human peripheral blood cells.

    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    Applications

    Our Abpromise guarantee covers the use of ab155360 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Flow Cyt Use 5µl for 106 cells.

    ab154457 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

    Target

    • Function

      Cooperates with MD-2 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules.

    • Tissue specificity

      Expressed strongly on the surface of monocytes and weakly on the surface of granulocytes; also expressed by most tissue macrophages.

    • Sequence similarities

      Contains 11 LRR (leucine-rich) repeats.

    • Post-translational
      modifications

      N- and O- glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan.

    • Cellular localization

      Cell membrane.
    • Information by UniProt

    • Database links

      • Alternative names

        • CD 14 antibody
        • CD_antigen=CD14 antibody
        • CD14 antibody
        • CD14 antigen antibody
        • CD14 molecule antibody
        • CD14_HUMAN antibody
        • LPS-R antibody
        • Mo2 antibody
        • Monocyte differentiation antigen CD14 antibody
        • Monocyte differentiation antigen CD14 urinary form antibody
        • Monocyte differentiation antigen CD14, membrane-bound form antibody
        • Myeloid cell specific leucine rich glycoprotein antibody
        • Myeloid cell-specific leucine-rich glycoprotein antibody
        see all

      Images

      • Flow Cytometry - PE/Cy7® Anti-CD14 antibody [61D3], prediluted (ab155360)
        Flow Cytometry - PE/Cy7® Anti-CD14 antibody [61D3], prediluted (ab155360)
        Flow cytometric analysis of normal Human peripheral blood cells with mouse IgG1 kappa Isotype Control PE-Cy7 (open histogram) or ab155360 (filled histogram). Cells in the monocyte gate were used for analysis.

      References (1)

      Publishing research using ab155360? Please let us know so that we can cite the reference in this datasheet.

      ab155360 has been referenced in 1 publication.

      • Vu T  et al. Reconfigurable microfluidic device with integrated antibody arrays for capture, multiplexed stimulation, and cytokine profiling of human monocytes. Biomicrofluidics 9:044115 (2015). PubMed: 26339315

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