PE/Cy7® Anti-CD16 antibody [3G8] (ab267386)
![Flow Cytometry - PE/Cy7® Anti-CD16 antibody [3G8] (ab267386)](https://www.abcam.com/ps/products/267/ab267386/Images/ab267386-365776-anti-cd16-antibody-3g8--pe-cy7-flow-cytometry-human.png "Flow Cytometry - PE/Cy7® Anti-CD16 antibody [3G8] (ab267386)")
Key features and details
- PE/Cy7® Mouse monoclonal [3G8] to CD16
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: PE/Cy7®. Ex: 496nm, Em: 774nm
- Isotype: IgG1
Overview
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Product name
PE/Cy7® Anti-CD16 antibody [3G8]
See all CD16 primary antibodies -
Description
PE/Cy7® Mouse monoclonal [3G8] to CD16 -
Host species
Mouse -
Conjugation
PE/Cy7®. Ex: 496nm, Em: 774nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
The details of the immunogen for this antibody are not available.
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Positive control
- Flow Cyt: Human peripheral blood lymphocytes.
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General notes
This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5, 268, 486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Store In the Dark. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituents: 0.16% Sodium phosphate, 0.87% Sodium chloride, 0.1% Gelatin -
Concentration information loading... -
Purity
Affinity purified -
Purification notes
The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation. -
Clonality
Monoclonal -
Clone number
3G8 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
- PE Anti-CD16 antibody [3G8] (ab117117)
- PerCP Anti-CD16 antibody [3G8] (ab130761)
- PerCP/Cy5.5® Anti-CD16 antibody [3G8], prediluted (ab157322)
- Anti-CD16 antibody [3G8] - Low endotoxin, Azide free (ab176528)
- Alexa Fluor® 488 Anti-CD16 antibody [3G8] (ab187585)
- APC/Cy7® Anti-CD16 antibody [3G8], prediluted (ab197141)
- PE/Cy7® Anti-CD16 antibody [3G8] (ab234209)
- FITC Anti-CD16 antibody [3G8] (ab239254)
- Alexa Fluor® 647 Anti-CD16 antibody [3G8] (ab239275)
Applications
Our Abpromise guarantee covers the use of ab267386 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use at an assay dependent concentration. The antibody has been diluted for use at 5 µL per test, defined as the amount of antibody that will stain a cell sample in a final volume of approximately 100 µL. The number of cells within a sample should be determined empirically, but typically ranges between 1x105 to 1x108 cells. |
Target
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Function
Receptor for the Fc region of IgG. Binds complexed or aggregated IgG and also monomeric IgG. Mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis. -
Tissue specificity
Expressed on natural killer cells, macrophages, subpopulation of T-cells, immature thymocytes and placental trophoblasts. -
Sequence similarities
Contains 2 Ig-like C2-type (immunoglobulin-like) domains. -
Post-translational
modificationsGlycosylated. Contains high mannose- and complex-type oligosaccharides.
The soluble form is produced by a proteolytic cleavage. -
Cellular localization
Cell membrane. Secreted. Exists also as a soluble receptor. - Information by UniProt
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Database links
- Entrez Gene: 2214 Human
- Omim: 146740 Human
- SwissProt: P08637 Human
- Unigene: 372679 Human
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Alternative names
- CD 16 antibody
- CD 16a antibody
- CD16 antibody
see all
Images
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Flow Cytometry - PE/Cy7® Anti-CD16 antibody [3G8] (ab267386)
Flow cytometric analysis of human peripheral blood lymphocytes stained for CD16 with 5 μL (0.5 μg) of ab267386 (Right) or 0.5 μg of a PE-Cy7®-labeled mouse IgG1 isotype control (Left).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab267386 has not yet been referenced specifically in any publications.
