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PE/Cy7® Anti-CD62L antibody [MEL-14] (ab25569)

  • Datasheet
  • SDS
Reviews (1) Submit a question References (4)

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Key features and details

  • PE/Cy7® Rat monoclonal [MEL-14] to CD62L
  • Reacts with: Mouse
  • Conjugation: PE/Cy7®. Ex: 496nm, Em: 774nm
  • Isotype: IgG2a

You may also be interested in

Protein
Product image
Recombinant Human CD62L protein (ab132972)
Primary
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FITC Anti-CD45RA antibody [MEM-56] (ab18240)

View more associated products

Overview

  • Product name

    PE/Cy7® Anti-CD62L antibody [MEL-14]
    See all CD62L primary antibodies
  • Description

    PE/Cy7® Rat monoclonal [MEL-14] to CD62L
  • Host species

    Rat
  • Conjugation

    PE/Cy7®. Ex: 496nm, Em: 774nm
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    Tissue/ cell preparation: C3H/eb cloned mouse B lymphoma 38C-13.

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.20
    Preservative: 0.09% Sodium azide
    Constituents: 0.142% Monobasic dihydrogen sodium phosphate, 0.87% Sodium chloride, 0.1% Gelatin
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

    Monoclonal
  • Clone number

    MEL-14
  • Isotype

    IgG2a
  • Light chain type

    kappa
  • Research areas

    • Cardiovascular
    • Blood
    • Other
    • Immunology
    • Adaptive Immunity
    • T Cells
    • CD
    • Stem Cells
    • Hematopoietic Progenitors
    • Lymphoid
    • T Lymphocytic Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Myeloid
    • Neutrophil Lineage
    • Immunology
    • Adaptive Immunity
    • Regulatory T Cells

Associated products

  • Alternative Versions

    • Anti-CD62L antibody [MEL-14] (ab119834)
    • redFluor™ 710 Anti-CD62L antibody [MEL-14] (ab242077)
    • FITC Anti-CD62L antibody [MEL-14] (ab24876)
    • Biotin Anti-CD62L antibody [MEL-14] (ab24969)
    • APC Anti-CD62L antibody [MEL-14] (ab41459)
  • Recombinant Protein

    • Recombinant Human CD62L protein (ab132972)

Target

  • Function

    Cell surface adhesion protein. Mediates the adherence of lymphocytes to endothelial cells of high endothelial venules in peripheral lymph nodes. Promotes initial tethering and rolling of leukocytes in endothelia.
  • Tissue specificity

    Expressed in B cell lines and T lymphocytes.
  • Sequence similarities

    Belongs to the selectin/LECAM family.
    Contains 1 C-type lectin domain.
    Contains 1 EGF-like domain.
    Contains 2 Sushi (CCP/SCR) domains.
  • Cellular localization

    Membrane.
  • Target information above from: UniProt accession P14151 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 20343 Mouse
    • SwissProt: P18337 Mouse
    • Unigene: 1461 Mouse
    • Alternative names

      • A.11 antibody
      • AI528707 antibody
      • CD62 antigen ligand antibody
      • CD62 antigen-like family member L antibody
      • CD62L antibody
      • gp90-MEL antibody
      • IgA nephropathy, susceptibility to, included antibody
      • L Selectin antibody
      • L-selectin antibody
      • LAM-1 antibody
      • LAM1 antibody
      • LECAM1 antibody
      • LEU8 antibody
      • Leukocyte adhesion molecule 1 antibody
      • Leukocyte surface antigen Leu-8 antibody
      • Leukocyte-endothelial cell adhesion molecule 1 antibody
      • Lnhr antibody
      • LSEL antibody
      • Ly-22 antibody
      • Ly-m22 antibody
      • Lyam-1 antibody
      • LYAM1 antibody
      • LYAM1_HUMAN antibody
      • Lymph node homing receptor antibody
      • Lymphocyte adhesion molecule 1 antibody
      • Lymphocyte antigen 22 antibody
      • Lymphocyte surface MEL-14 antigen antibody
      • MEL-14 antibody
      • Pln homing receptor antibody
      • PLNHR antibody
      • Selectin L antibody
      • Selectin, lymphocyte antibody
      • SELL antibody
      • TQ1 antibody
      see all

    Protocols

    • Flow cytometry protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (4)

    Publishing research using ab25569? Please let us know so that we can cite the reference in this datasheet.

    ab25569 has been referenced in 4 publications.

    • Quinn KM  et al. Antigen expression determines adenoviral vaccine potency independent of IFN and STING signaling. J Clin Invest 125:1129-46 (2015). PubMed: 25642773
    • Quinn KM  et al. Comparative analysis of the magnitude, quality, phenotype, and protective capacity of simian immunodeficiency virus gag-specific CD8+ T cells following human-, simian-, and chimpanzee-derived recombinant adenoviral vector immunization. J Immunol 190:2720-35 (2013). Mouse . PubMed: 23390298
    • Quinn KM  et al. Coadministration of polyinosinic:polycytidylic acid and immunostimulatory complexes modifies antigen processing in dendritic cell subsets and enhances HIV gag-specific T cell immunity. J Immunol 191:5085-96 (2013). ICC/IF . PubMed: 24089189
    • Jones M  et al. IL-10 Restricts Memory T Cell Inflation during Cytomegalovirus Infection. J Immunol 185:3583-92 (2010). Flow Cyt ; Mouse . PubMed: 20713884

    Customer reviews and Q&As

    Show All Reviews Q&A
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    Flow Cytometry abreview for Anti-CD62L antibody [MEL-14] (PE/Cy7 ®)

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Sample
    Mouse Cell (whole blood)
    Specification
    whole blood
    Fixation
    none
    Permeabilization
    No
    Gating Strategy
    monocyte, lymphocyte and granulocyte
    Read More

    Abcam user community

    Verified customer

    Submitted May 01 2012

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