PE/Cy7® Anti-CD69 antibody [FN50] (ab135930)

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Flow Cytometry - PE/Cy7® Anti-CD69 antibody [FN50] (ab135930)

    Key features and details

    • PE/Cy7® Mouse monoclonal [FN50] to CD69
    • Suitable for: Flow Cyt
    • Reacts with: Human
    • Conjugation: PE/Cy7®. Ex: 496nm, Em: 774nm
    • Isotype: IgG1

    Overview

    • Product name

      PE/Cy7® Anti-CD69 antibody [FN50]
      See all CD69 primary antibodies

    • Description

      PE/Cy7® Mouse monoclonal [FN50] to CD69

    • Host species

      Mouse

    • Conjugation

      PE/Cy7®. Ex: 496nm, Em: 774nm

    • Tested applications

      Suitable for: Flow Cytmore details

    • Species reactivity

      Reacts with: Human

    • Immunogen

      The details of the immunogen for this antibody are not available.

    • Positive control

      • PHA stimulated normal human peripheral blood cells.

    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    Applications

    Our Abpromise guarantee covers the use of ab135930 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Flow Cyt Use 5µl for 106 cells.

    ab154457 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

    Target

    • Function

      Involved in lymphocyte proliferation and functions as a signal transmitting receptor in lymphocytes, natural killer (NK) cells, and platelets.

    • Tissue specificity

      Expressed on the surface of activated T-cells, B-cells, natural killer cells, neutrophils, eosinophils, epidermal Langerhans cells and platelets.

    • Sequence similarities

      Contains 1 C-type lectin domain.

    • Developmental stage

      Earliest inducible cell surface glycoprotein acquired during lymphoid activation.

    • Post-translational
      modifications

      Constitutive Ser/Thr phosphorylation in both mature thymocytes and activated T-lymphocytes.

    • Cellular localization

      Membrane.
    • Information by UniProt

    • Database links

      • Alternative names

        • Activation inducer molecule (AIM/CD69) antibody
        • Activation inducer molecule antibody
        • AIM antibody
        • BL-AC/P26 antibody
        • BLAC/P26 antibody
        • C-type lectin domain family 2 member C antibody
        • CD69 antibody
        • CD69 antigen (p60, early T-cell activation antigen) antibody
        • CD69 antigen antibody
        • CD69 molecule antibody
        • CD69_HUMAN antibody
        • CLEC2C antibody
        • EA1 antibody
        • Early activation antigen CD69 antibody
        • Early lymphocyte activation antigen antibody
        • Early T cell activation antigen p60 antibody
        • Early T-cell activation antigen p60 antibody
        • GP32/28 antibody
        • Leu23 antibody
        • Leukocyte surface antigen Leu-23 antibody
        • MLR-3 antibody
        • MLR3 antibody
        • VEA antibody
        • Very Early Activation Antigen antibody
        see all

      Images

      • Flow Cytometry - PE/Cy7® Anti-CD69 antibody [FN50] (ab135930)
        Flow Cytometry - PE/Cy7® Anti-CD69 antibody [FN50] (ab135930)
        Flow cytometric analysis of PHA stimulated normal Human peripheral blood cells labelling CD69 with either Mouse IgG1 kappa Isotype Control PE/Cy7 (open histogram) or ab135930 at 5µl (filled histogram). Total cells were used for analysis.

      References (0)

      Publishing research using ab135930? Please let us know so that we can cite the reference in this datasheet.

      ab135930 has not yet been referenced specifically in any publications.

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