PE/Cy7® Anti-CD69 antibody [H1.2F3] (ab25529)
![Flow Cytometry - PE/Cy7® Anti-CD69 antibody [H1.2F3] (ab25529)](https://www.abcam.com/ps/products/25/ab25529/Images/ab25529-284720-anti-cd69-antibody-h12f3-pe-cy7-flow-cytometry.jpg "Flow Cytometry - PE/Cy7® Anti-CD69 antibody [H1.2F3] (ab25529)")
Key features and details
- PE/Cy7® Armenian hamster monoclonal [H1.2F3] to CD69
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: PE/Cy7®. Ex: 496nm, Em: 774nm
- Isotype: IgG
Related conjugates and formulations
Overview
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Product name
PE/Cy7® Anti-CD69 antibody [H1.2F3]
See all CD69 primary antibodies -
Description
PE/Cy7® Armenian hamster monoclonal [H1.2F3] to CD69 -
Host species
Armenian hamster -
Conjugation
PE/Cy7®. Ex: 496nm, Em: 774nm -
Specificity
ab25529 recognises mouse CD69/Very Early Activation Antigen (VEA). -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Tissue, cells or virus corresponding to Mouse CD69. Mouse dendritic epidermal cell line Y245
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General notes
This antibody augments PMA-induced T-cell proliferation, and induces redirected lysis of Fc receptor-bearing target cells by NK cells.
This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5, 268, 486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.1
Preservative: 0.09% Sodium azide
Constituents: PBS, 16% Sucrose
Also contains a stabilizing agent. -
Concentration information loading... -
Purity
Affinity purified -
Primary antibody notes
This antibody augments PMA-induced T-cell proliferation, and induces redirected lysis of Fc receptor-bearing target cells by NK cells. -
Clonality
Monoclonal -
Clone number
H1.2F3 -
Isotype
IgG -
Light chain type
lambda -
Research areas
Associated products
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Alternative Versions
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab25529 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
Use 0.2µg for 106 cells.
|
| Notes |
|---|
|
Flow Cyt
Use 0.2µg for 106 cells. |
Target
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Function
Involved in lymphocyte proliferation and functions as a signal transmitting receptor in lymphocytes, natural killer (NK) cells, and platelets. -
Tissue specificity
Expressed on the surface of activated T-cells, B-cells, natural killer cells, neutrophils, eosinophils, epidermal Langerhans cells and platelets. -
Sequence similarities
Contains 1 C-type lectin domain. -
Developmental stage
Earliest inducible cell surface glycoprotein acquired during lymphoid activation. -
Post-translational
modificationsConstitutive Ser/Thr phosphorylation in both mature thymocytes and activated T-lymphocytes. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 12515 Mouse
- SwissProt: P37217 Mouse
- Unigene: 74745 Mouse
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Alternative names
- Activation inducer molecule (AIM/CD69) antibody
- Activation inducer molecule antibody
- AIM antibody
see all
Images
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Flow Cytometry - PE/Cy7® Anti-CD69 antibody [H1.2F3] (ab25529)
Flow cytometry analysis of Con-A stimulated BALB/c mouse splenocutes with ab25529.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab25529 has not yet been referenced specifically in any publications.