PE/Cy7® Anti-CD73 antibody [AD2] (ab253253)

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Flow Cytometry - PE/Cy7® Anti-CD73 antibody [AD2] (ab253253)

    Key features and details

    • PE/Cy7® Mouse monoclonal [AD2] to CD73
    • Suitable for: Flow Cyt
    • Conjugation: PE/Cy7®. Ex: 496nm, Em: 774nm
    • Isotype: IgG1

    Overview

    • Product name

      PE/Cy7® Anti-CD73 antibody [AD2]
      See all CD73 primary antibodies

    • Description

      PE/Cy7® Mouse monoclonal [AD2] to CD73

    • Host species

      Mouse

    • Conjugation

      PE/Cy7®. Ex: 496nm, Em: 774nm

    • Tested applications

      Suitable for: Flow Cytmore details

    • Immunogen

      The details of the immunogen for this antibody are not available.

    • Positive control

      • Flow Cyt: Human peripheral bood cells.

    • General notes

      This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5, 268, 486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited.

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid

    • Storage instructions

      Shipped at 4°C. Store at +4°C. Store In the Dark.

    • Storage buffer

      pH: 7.4
      Preservative: 0.0975% Sodium azide
      Constituent: PBS
    • Concentration information loading...

    • Purity

      Size exclusion

    • Purification notes

      The purified antibody is conjugated with tandem dye PE-Cy™7 under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use.

    • Clonality

      Monoclonal

    • Clone number

      AD2

    • Isotype

      IgG1

    • Research areas

    Applications

    Our Abpromise guarantee covers the use of ab253253 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Flow Cyt Use 4µl for 106 cells.

    Or 4 μL per 100 μL whole blood.

    Target

    • Function

      Hydrolyzes extracellular nucleotides into membrane permeable nucleosides.

    • Involvement in disease

      Defects in NT5E are the cause of calcification of joints and arteries (CAJA) [MIM:211800]. A condition characterized by adult-onset calcification of the lower extremity arteries, including the iliac, femoral and tibial arteries, and hand and foot capsule joints. Age of onset has been reported as early as the second decade of life, usually involving intense joint pain or calcification in the hands.

    • Sequence similarities

      Belongs to the 5'-nucleotidase family.

    • Cellular localization

      Cell membrane.
    • Information by UniProt

    • Alternative names

      • 5' NT antibody
      • 5' nucleotidase (CD73) antibody
      • 5' nucleotidase precursor antibody
      • 5' nucleotidase, ecto antibody
      • 5' nucleotidase, ecto (CD73) antibody
      • 5'-NT antibody
      • 5'-nucleotidase antibody
      • 5NTD_HUMAN antibody
      • CD73 antibody
      • CD73 antigen antibody
      • E5NT antibody
      • Ecto 5' nucleotidase antibody
      • Ecto-5'-nucleotidase antibody
      • eN antibody
      • eNT antibody
      • NT antibody
      • NT5 antibody
      • NT5E antibody
      • NTE antibody
      • Purine 5 Prime Nucleotidase antibody
      see all

    Images

    • Flow Cytometry - PE/Cy7® Anti-CD73 antibody [AD2] (ab253253)
      Flow Cytometry - PE/Cy7® Anti-CD73 antibody [AD2] (ab253253)

      Flow cytometric analysis of human peripheral blood cells labeling CD73 with ab253253.

      Surface staining.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab253253? Please let us know so that we can cite the reference in this datasheet.

    ab253253 has not yet been referenced specifically in any publications.

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