PE/Cy7® Anti-CD79a antibody [HM47] (ab253255)
![Flow Cytometry - PE/Cy7® Anti-CD79a antibody [HM47] (ab253255)](https://www.abcam.com/ps/products/253/ab253255/Images/ab253255-343937-anticd79aantibodyhm47-flowcytometry-blood-human.jpg "Flow Cytometry - PE/Cy7® Anti-CD79a antibody [HM47] (ab253255)")
Key features and details
- PE/Cy7® Mouse monoclonal [HM47] to CD79a
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: PE/Cy7®. Ex: 496nm, Em: 774nm
- Isotype: IgG1
Overview
-
Product name
PE/Cy7® Anti-CD79a antibody [HM47]
See all CD79a primary antibodies -
Description
PE/Cy7® Mouse monoclonal [HM47] to CD79a -
Host species
Mouse -
Conjugation
PE/Cy7®. Ex: 496nm, Em: 774nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human CD79a aa 208-222 (C terminal).
Sequence:GTYQDVGSLNIGDVQL
Database link: P11912 -
Positive control
- Flow cyt: Human peripheral blood.
-
General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Store In the Dark. -
Storage buffer
pH: 7.4
Preservative: 0.0975% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Size exclusion -
Clonality
Monoclonal -
Clone number
HM47 -
Isotype
IgG1 -
Research areas
Associated products
-
Alternative Versions
-
Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab253255 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use 4µl for 106 cells. Or 4 μL per 100 μL whole blood. |
Target
-
Function
Required in cooperation with CD79B for initiation of the signal transduction cascade activated by binding of antigen to the B-cell antigen receptor complex (BCR) which leads to internalization of the complex, trafficking to late endosomes and antigen presentation. Also required for BCR surface expression and for efficient differentiation of pro- and pre-B-cells. Stimulates SYK autophosphorylation and activation. Binds to BLNK, bringing BLNK into proximity with SYK and allowing SYK to phosphorylate BLNK. Also interacts with and increases activity of some Src-family tyrosine kinases. Represses BCR signaling during development of immature B cells. -
Tissue specificity
B-cells. -
Involvement in disease
Defects in CD79A are the cause of agammaglobulinemia type 3 (AGM3) [MIM:613501]. It is a primary immunodeficiency characterized by profoundly low or absent serum antibodies and low or absent circulating B cells due to an early block of B-cell development. Affected individuals develop severe infections in the first years of life. Note=Two different mutations, one at the splice donor site of intron 2 and the other at the splice acceptor site for exon 3, have been identified. Both mutations give rise to a truncated protein. -
Sequence similarities
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 ITAM domain. -
Post-translational
modificationsPhosphorylated on tyrosine, serine and threonine residues upon B-cell activation. Phosphorylation of tyrosine residues by Src-family kinases is an early and essential feature of the BCR signaling cascade. The phosphorylated tyrosines serve as docking sites for SH2-domain containing kinases, leading to their activation which in turn leads to phosphorylation of downstream targets. Phosphorylation of serine and threonine residues may prevent subsequent tyrosine phosphorylation. -
Cellular localization
Cell membrane. Following antigen binding, the BCR has been shown to translocate from detergent-soluble regions of the cell membrane to lipid rafts although signal transduction through the complex can also occur outside lipid rafts. - Information by UniProt
-
Database links
- Entrez Gene: 973 Human
- Omim: 112205 Human
- SwissProt: P11912 Human
- Unigene: 631567 Human
-
Alternative names
- B lymphocyte-specific MB1 protein antibody
- B-cell antigen receptor complex-associated protein alpha chain antibody
- CD 79a antibody
see all
Images
-
Flow Cytometry - PE/Cy7® Anti-CD79a antibody [HM47] (ab253255)
Intracellular staining of CD79a in human peripheral blood using ab253255.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab253255 has not yet been referenced specifically in any publications.
