PE/Cy7® Anti-CD8 alpha antibody [2.43] (ab210216)
![Flow Cytometry - PE/Cy7® Anti-CD8 alpha antibody [2.43] (ab210216)](https://www.abcam.com/ps/products/210/ab210216/Images/ab210216-256503-anti-cd8-alpha-antibody-243-pe-cy7-flow-cytometry.png "Flow Cytometry - PE/Cy7® Anti-CD8 alpha antibody [2.43] (ab210216)")
Key features and details
- PE/Cy7® Rat monoclonal [2.43] to CD8 alpha
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: PE/Cy7®. Ex: 496nm, Em: 774nm
- Isotype: IgG2b
Overview
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Product name
PE/Cy7® Anti-CD8 alpha antibody [2.43]
See all CD8 alpha primary antibodies -
Description
PE/Cy7® Rat monoclonal [2.43] to CD8 alpha -
Host species
Rat -
Conjugation
PE/Cy7®. Ex: 496nm, Em: 774nm -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt Mouse
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Positive control
- Mouse spleen cells
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General notes
The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.
This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5, 268, 486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. Store In the Dark. Store undiluted. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituents: 0.12% Monobasic dihydrogen sodium phosphate, 0.87% Sodium chloride, 0.1% Gelatin -
Concentration information loading... -
Purification notes
ab210216 was purified from tissue culture supernatant via affinity chromatography. -
Clonality
Monoclonal -
Clone number
2.43 -
Isotype
IgG2b -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab210216 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| Flow Cyt |
Mouse
|
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
Use at an assay dependent concentration.
Use 0.25 µg |
| Notes |
|---|
|
Flow Cyt
Use at an assay dependent concentration. Use 0.25 µg |
Target
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Function
Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains. -
Involvement in disease
Defects in CD8A are a cause of familial CD8 deficiency (CD8 deficiency) [MIM:608957]. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections. -
Sequence similarities
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Post-translational
modificationsAll of the five most carboxyl-terminal cysteines form inter-chain disulfide bonds in dimers and higher multimers, while the four N-terminal cysteines do not. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 12525 Mouse
- SwissProt: P01731 Mouse
- Unigene: 1858 Mouse
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Alternative names
- alpha polypeptide (p32) antibody
- CD_antigen=CD8a antibody
- CD8 antibody
see all
Images
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Flow Cytometry - PE/Cy7® Anti-CD8 alpha antibody [2.43] (ab210216)
Flow cytometric analysis of C57Bl/6 splenocytes labeling CD8 alpha with ab210216 at 0.25 μg (solid line) or Rat IgG2b PE-Cy7 isotype control at 0.25 μg (dashed line).
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab210216 has not yet been referenced specifically in any publications.