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  1. Link

    pecy7-epcam-antibody-vu-1d9-ab239311.pdf

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Tags & Cell Markers Cell Type Markers Tumor Associated
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PE/Cy7® Anti-EpCAM antibody [VU-1D9] (ab239311)

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Flow Cytometry - PE/Cy7® Anti-EpCAM antibody [VU-1D9] (ab239311)
  • Flow Cytometry - PE/Cy7® Anti-EpCAM antibody [VU-1D9] (ab239311)

Key features and details

  • PE/Cy7® Mouse monoclonal [VU-1D9] to EpCAM
  • Suitable for: Flow Cyt
  • Reacts with: Human
  • Conjugation: PE/Cy7®. Ex: 496nm, Em: 774nm
  • Isotype: IgG1

Overview

  • Product name

    PE/Cy7® Anti-EpCAM antibody [VU-1D9]
    See all EpCAM primary antibodies
  • Description

    PE/Cy7® Mouse monoclonal [VU-1D9] to EpCAM
  • Host species

    Mouse
  • Conjugation

    PE/Cy7®. Ex: 496nm, Em: 774nm
  • Tested applications

    Suitable for: Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Tissue, cells or virus corresponding to Human EpCAM. (Small cell lung carcinoma cell line H69).

  • Epitope

    The mouse monoclonal antibody VU-1D9 recognizes an extracellular epitope within EGF-like domain I of CD326 / EpCAM.
  • Positive control

    • Flow Cyt: MCF7 cells.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Store In the Dark.
  • Storage buffer

    pH: 7.4
    Preservative: 0.0975% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Size exclusion
  • Purification notes

    Purified from TCS. Purified antibody is conjugated with tandem dye PE/Cy7 ® under optimum conditions. The conjugate is purified by size-exclusion chromatography.
  • Clonality

    Monoclonal
  • Clone number

    VU-1D9
  • Isotype

    IgG1
  • Research areas

    • Tags & Cell Markers
    • Cell Type Markers
    • Tumor Associated
    • Tags & Cell Markers
    • Cell Type Markers
    • Epi / Endo-thelial

Applications

Our Abpromise guarantee covers the use of ab239311 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 4µl for 106 cells.

or 100 μl of whole blood.

Target

  • Function

    May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E.
  • Tissue specificity

    Highly and selectively expressed by undifferentiated rather than differentiated embryonic stem cells (ESC). Levels rapidly diminish as soon as ESC's differentiate (at protein levels). Expressed in almost all epithelial cell membranes but not on mesodermal or neural cell membranes. Found on the surface of adenocarcinoma.
  • Involvement in disease

    Defects in EPCAM are the cause of diarrhea type 5 (DIAR5) [MIM:613217]. It is an intractable diarrhea of infancy characterized by villous atrophy and absence of inflammation, with intestinal epithelial cell dysplasia manifesting as focal epithelial tufts in the duodenum and jejunum.
    Defects in EPCAM are a cause of hereditary non-polyposis colorectal cancer type 8 (HNPCC8) [MIM:613244]. HNPCC is a disease associated with marked increase in cancer susceptibility. It is characterized by a familial predisposition to early-onset colorectal carcinoma (CRC) and extra-colonic tumors of the gastrointestinal, urological and female reproductive tracts. HNPCC is reported to be the most common form of inherited colorectal cancer in the Western world. Clinically, HNPCC is often divided into two subgroups. Type I is characterized by hereditary predisposition to colorectal cancer, a young age of onset, and carcinoma observed in the proximal colon. Type II is characterized by increased risk for cancers in certain tissues such as the uterus, ovary, breast, stomach, small intestine, skin, and larynx in addition to the colon. Diagnosis of classical HNPCC is based on the Amsterdam criteria: 3 or more relatives affected by colorectal cancer, one a first degree relative of the other two; 2 or more generation affected; 1 or more colorectal cancers presenting before 50 years of age; exclusion of hereditary polyposis syndromes. The term 'suspected HNPCC' or 'incomplete HNPCC' can be used to describe families who do not or only partially fulfill the Amsterdam criteria, but in whom a genetic basis for colon cancer is strongly suspected. Note=HNPCC8 results from heterozygous deletion of 3-prime exons of EPCAM and intergenic regions directly upstream of MSH2, resulting in transcriptional read-through and epigenetic silencing of MSH2 in tissues expressing EPCAM.
  • Sequence similarities

    Belongs to the EPCAM family.
    Contains 1 thyroglobulin type-1 domain.
  • Post-translational
    modifications

    Hyperglycosylated in carcinoma tissue as compared with autologous normal epithelia. Glycosylation at Asn-198 is crucial for protein stability.
  • Cellular localization

    Lateral cell membrane. Cell junction > tight junction. Co-localizes with CLDN7 at the lateral cell membrane and tight junction.
  • Target information above from: UniProt accession P16422 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 4072 Human
    • Omim: 185535 Human
    • SwissProt: P16422 Human
    • Unigene: 542050 Human
    • Alternative names

      • 17 1A antibody
      • 323/A3 antibody
      • Adenocarcinoma associated antigen antibody
      • Adenocarcinoma-associated antigen antibody
      • Antigen identified by monoclonal antibody AUA1 antibody
      • AUA1 antibody
      • CD326 antibody
      • CD326 antigen antibody
      • Cell surface glycoprotein Trop 1 antibody
      • Cell surface glycoprotein Trop 2 antibody
      • Cell surface glycoprotein Trop-1 antibody
      • CO 17A antibody
      • CO17 1A antibody
      • CO17A antibody
      • DIAR5 antibody
      • EGP 2 antibody
      • EGP antibody
      • EGP2 antibody
      • EGP314 antibody
      • EGP40 antibody
      • Ep CAM antibody
      • Ep-CAM antibody
      • EPCAM antibody
      • EPCAM_HUMAN antibody
      • EpCAM1 antibody
      • Epithelial cell adhesion molecule antibody
      • Epithelial Cell Adhesion Molecule Intracellular Domain (EpCAM-ICD) antibody
      • Epithelial cell surface antigen antibody
      • Epithelial cellular adhesion molecule antibody
      • Epithelial glycoprotein 1 antibody
      • Epithelial glycoprotein 314 antibody
      • Epithelial glycoprotein antibody
      • ESA antibody
      • GA733 1 antibody
      • GA733 2 antibody
      • GA733-2 antibody
      • gastrointestinal tumor-associated antigen 2, 35-KD glycoprotein antibody
      • gp4 antibody
      • hEGP 2 antibody
      • hEGP314 antibody
      • HNPCC8 antibody
      • Human epithelial glycoprotein 2 antibody
      • KS 1/4 antigen antibody
      • KS1/4 antibody
      • KSA antibody
      • Ly74 antibody
      • Lymphocyte antigen 74 antibody
      • M1S 1 antibody
      • M1S2 antibody
      • M4S1 antibody
      • Major gastrointestinal tumor associated protein GA733 2 antibody
      • Major gastrointestinal tumor-associated protein GA733-2 antibody
      • mEGP314 antibody
      • Membrane component chromosome 4 surface marker (35kD glycoprotein) antibody
      • Membrane component, chromosome 4, surface marker 1 antibody
      • Membrane component, chromosome 4, surface marker antibody
      • MIC18 antibody
      • MK 1 antibody
      • Protein 289A antibody
      • TACD1 antibody
      • TACSTD1 antibody
      • TROP1 antibody
      • Tumor associated calcium signal transducer 1 antibody
      • Tumor associated calcium signal transducer 2 precursor antibody
      • Tumor-associated calcium signal transducer 1 antibody
      see all

    Images

    • Flow Cytometry - PE/Cy7® Anti-EpCAM antibody [VU-1D9] (ab239311)
      Flow Cytometry - PE/Cy7® Anti-EpCAM antibody [VU-1D9] (ab239311)

      Flow cytometry analysis of MCF7 (human breast adenocarcinoma cell line) cells labeling EpCAM with ab239311 at 4 μl reagent per milion cells in 100 μl of cell suspension. Surface staining.

    • Flow Cytometry - PE/Cy7® Anti-EpCAM antibody [VU-1D9] (ab239311)
      Flow Cytometry - PE/Cy7® Anti-EpCAM antibody [VU-1D9] (ab239311)

      Separation of MCF7 cells (red-filled) from SP2 cells (black-dashed) in flow cytometry analysis labeling EpCAM with ab239311 at 4 μl reagent per milion cells in 100 μl of cell suspension. Surface staining.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab239311? Please let us know so that we can cite the reference in this datasheet.

    ab239311 has not yet been referenced specifically in any publications.

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